Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans

Polyhydroxyalkanoates (PHAs) are biodegradable polyesters which are stored in bacterial cell cytoplasm as reserve materials for carbon and energy. One of the main problems of plastics is that they have resistance to biological breakdown that result in accumulation in the environment. Bacteria synthe...

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Main Author:	Mojaveryazdi, Farzaneh Sabbagh
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/33257/5/FarzanehSabbaghMFBB2013.pdf
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spelling	my-utm-ep.332572017-07-24T01:12:15Z Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans 2013-02 Mojaveryazdi, Farzaneh Sabbagh Q Science (General) Polyhydroxyalkanoates (PHAs) are biodegradable polyesters which are stored in bacterial cell cytoplasm as reserve materials for carbon and energy. One of the main problems of plastics is that they have resistance to biological breakdown that result in accumulation in the environment. Bacteria synthesize and accumulate polyhydroxyalkanote (PHA) as carbon source under limiting conditions of nutrients. In this study, the bacteria, P. oleovorans was studied for its ability to produce PHA in the minimal basal medium supplemented with glucose as carbon source and (NH4)2SO3 as nitrogen source that was grown at 25oC. The functional groups of the extracted PHA granules were identified as a C=O group by Fourier Transform Infrared (FTIR) spectroscopy analysis. The drastic absorption band at approximately 1720 cm-1 indicated the stretching vibration of the C=O groups in the PHA polyester. The influence of different carbon sources, nitrogen sources, pH and inoculums on PHA production was investigated. The production optimization of PHA was done by RSM (Response Surface Method) through various growth parameters. After optimization obtained the best condition of productivity in range are pH 5.9, carbon source 93.4419 g/l, inoculums size 2.07% and nitrogen source 101.691 g/l. Also the highest PHA production after optimization is 2.28236 g/L with a desirability of 0.986 g/l, meanwhile the highest amount of PHA produced from P. oleovorans was 2.30 g/l. 2013-02 Thesis http://eprints.utm.my/id/eprint/33257/ http://eprints.utm.my/id/eprint/33257/5/FarzanehSabbaghMFBB2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mojaveryazdi, Farzaneh Sabbagh Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans
description	Polyhydroxyalkanoates (PHAs) are biodegradable polyesters which are stored in bacterial cell cytoplasm as reserve materials for carbon and energy. One of the main problems of plastics is that they have resistance to biological breakdown that result in accumulation in the environment. Bacteria synthesize and accumulate polyhydroxyalkanote (PHA) as carbon source under limiting conditions of nutrients. In this study, the bacteria, P. oleovorans was studied for its ability to produce PHA in the minimal basal medium supplemented with glucose as carbon source and (NH4)2SO3 as nitrogen source that was grown at 25oC. The functional groups of the extracted PHA granules were identified as a C=O group by Fourier Transform Infrared (FTIR) spectroscopy analysis. The drastic absorption band at approximately 1720 cm-1 indicated the stretching vibration of the C=O groups in the PHA polyester. The influence of different carbon sources, nitrogen sources, pH and inoculums on PHA production was investigated. The production optimization of PHA was done by RSM (Response Surface Method) through various growth parameters. After optimization obtained the best condition of productivity in range are pH 5.9, carbon source 93.4419 g/l, inoculums size 2.07% and nitrogen source 101.691 g/l. Also the highest PHA production after optimization is 2.28236 g/L with a desirability of 0.986 g/l, meanwhile the highest amount of PHA produced from P. oleovorans was 2.30 g/l.
format	Thesis
qualification_level	Master's degree
author	Mojaveryazdi, Farzaneh Sabbagh
author_facet	Mojaveryazdi, Farzaneh Sabbagh
author_sort	Mojaveryazdi, Farzaneh Sabbagh
title	Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans
title_short	Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans
title_full	Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans
title_fullStr	Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans
title_full_unstemmed	Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans
title_sort	production of polyhydroxyalkanate (pha) from waste cooking oil using pseudomonas oleovorans
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/33257/5/FarzanehSabbaghMFBB2013.pdf
_version_	1747816117576925184

Production of polyhydroxyalkanate (PHA) from waste cooking oil using pseudomonas oleovorans

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