Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato

Tomato (Lycopersicon esculentum Mill.) is a major vegetable crop that has achieved tremendous popularity over the last century. However, its production faced many problems like diseases, therefore, establishing a good protocol to improve the quantity and the quality of the crops is a prerequisite no...

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Main Author:	Hamad Ameen, Hazhar Muhammad
Format:	Thesis
Language:	English
Published:	2013
Subjects:	SB Plant culture
Online Access:	http://eprints.utm.my/id/eprint/33838/5/HazharMuhammadHamadMFBB2013.pdf
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id	my-utm-ep.33838
record_format	uketd_dc
spelling	my-utm-ep.338382021-07-27T09:08:19Z Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato 2013-01 Hamad Ameen, Hazhar Muhammad SB Plant culture Tomato (Lycopersicon esculentum Mill.) is a major vegetable crop that has achieved tremendous popularity over the last century. However, its production faced many problems like diseases, therefore, establishing a good protocol to improve the quantity and the quality of the crops is a prerequisite nowadays. Moreover, tissue culture has provided rapid modes to develop desirable varieties of cultivated plant species. In this study, in vitro culture response was assessed in tomato (Lycopersicon esculentum Mill. c.v. King Stone) for in vitro germination and optimum callus induction. The highest germination frequency (84.44%) was obtained on MS media (maltose as carbon source). In addition, to optimize an efficient protocol for callus induction, MS medium were supplemented with different source of carbon (sucrose and maltose) and different concentration of 2,4-D (0, 0.25, 0.5, 0.75, 1) mg/l in combination with BAP 1.5 mg/l and GA3 0.5 mg/l. In cotyledon explants the highest response of callus induction frequency (81.25%) and callus induction rate (3.25) were in MS media, which was supplemented with sucrose as carbon source and plant growth regulators 2,4-D 0 mg/l, BAP 1.5 mg/l and GA3 0.5 mg/l (T1). while the maximum callus induction frequency (100%), callus induction rate (4.54) and explant productivity (17.32%) were obtained from hypocotyl explants which cultured on MS media in T1. In conclusion, maximum in vitro seed germination was observed on MS medium supplemented with maltose. While sucrose shows a significant role in callus induction. Callus induction was observed in both hypocotyl and cotyledon explants. Hypocotyls showed to be better explants for callogenesis. Maximum callogenesis was noted on MS medium supplemented with 2,4-D (0 mg/l), BAP (1.5 mg/l), GA3 (0.5 mg/l). 2013-01 Thesis http://eprints.utm.my/id/eprint/33838/ http://eprints.utm.my/id/eprint/33838/5/HazharMuhammadHamadMFBB2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Hamad Ameen, Hazhar Muhammad Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato
description	Tomato (Lycopersicon esculentum Mill.) is a major vegetable crop that has achieved tremendous popularity over the last century. However, its production faced many problems like diseases, therefore, establishing a good protocol to improve the quantity and the quality of the crops is a prerequisite nowadays. Moreover, tissue culture has provided rapid modes to develop desirable varieties of cultivated plant species. In this study, in vitro culture response was assessed in tomato (Lycopersicon esculentum Mill. c.v. King Stone) for in vitro germination and optimum callus induction. The highest germination frequency (84.44%) was obtained on MS media (maltose as carbon source). In addition, to optimize an efficient protocol for callus induction, MS medium were supplemented with different source of carbon (sucrose and maltose) and different concentration of 2,4-D (0, 0.25, 0.5, 0.75, 1) mg/l in combination with BAP 1.5 mg/l and GA3 0.5 mg/l. In cotyledon explants the highest response of callus induction frequency (81.25%) and callus induction rate (3.25) were in MS media, which was supplemented with sucrose as carbon source and plant growth regulators 2,4-D 0 mg/l, BAP 1.5 mg/l and GA3 0.5 mg/l (T1). while the maximum callus induction frequency (100%), callus induction rate (4.54) and explant productivity (17.32%) were obtained from hypocotyl explants which cultured on MS media in T1. In conclusion, maximum in vitro seed germination was observed on MS medium supplemented with maltose. While sucrose shows a significant role in callus induction. Callus induction was observed in both hypocotyl and cotyledon explants. Hypocotyls showed to be better explants for callogenesis. Maximum callogenesis was noted on MS medium supplemented with 2,4-D (0 mg/l), BAP (1.5 mg/l), GA3 (0.5 mg/l).
format	Thesis
qualification_level	Master's degree
author	Hamad Ameen, Hazhar Muhammad
author_facet	Hamad Ameen, Hazhar Muhammad
author_sort	Hamad Ameen, Hazhar Muhammad
title	Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato
title_short	Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato
title_full	Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato
title_fullStr	Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato
title_full_unstemmed	Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato
title_sort	studies to investigate the effect of plant growth regulators, carbon sources and media modification on in vutro seed germination and callogenesis in tomato
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/33838/5/HazharMuhammadHamadMFBB2013.pdf
_version_	1747816197552865280

Studies to investigate the effect of plant growth regulators, carbon sources and media modification on in Vutro seed germination and callogenesis in tomato

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