Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts

Ficus deltoidea and Labisia pumila are two local plants that have been used in traditional medicine for along time for enhancement reproductive system and cancer treatment. In this study cell based assay were used to determine the effect of aqueous extract of these two plants on the Human Prostate c...

Full description

Saved in:

Bibliographic Details
Main Author:	Omer, Kosar Ali
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QK Botany
Online Access:	http://eprints.utm.my/id/eprint/34684/1/KosarAliAmerMFBB2013.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.34684
record_format	uketd_dc
spelling	my-utm-ep.346842017-09-14T06:39:24Z Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts 2013-01 Omer, Kosar Ali QK Botany Ficus deltoidea and Labisia pumila are two local plants that have been used in traditional medicine for along time for enhancement reproductive system and cancer treatment. In this study cell based assay were used to determine the effect of aqueous extract of these two plants on the Human Prostate carcinoma (DU145) cell line. MTT assay, viability test and morphological studies were carried out. Ficus deltoidea showed higher growth inhibitive than Labisia pumila extract. The MTT assay indicated that the most inhibitive concentration of the extracts is 1*10-3 µg/ml, this concentration is consistent through out the course of this study. MTT assay demonstrated that Ficus deltoidea extracts killed around 60% and Labisia pumila around 42% of the cells compared with the negative control. In the viability test Ficus deltoida also recorded a better effect than Labisia pumila which killed all the cells with in 7 days. In the case of Labisia pumila extract is took 9 days, while 12 days needed for negative control. Both extract showed almost similar effect on the morphology of the cells. Observation under inverted microscope for both extracts observed irregular detachment and clumping of the cells. There was morphological changing of the cells from hexagonal to round shape. In apoptotic body study the effect was similar in blabbing cells, chromatin condensation, apoptotic cells and late apoptotic body formation, the difference was in the living cells which for Labisia pumila extract more living cells seen than Ficus deltoidea extract. In general both aqueous extracts showed a growth suppressive effect on the DU145 cell line, but Ficus deltoidea extract was more effective than Labisia pumila extract. 2013-01 Thesis http://eprints.utm.my/id/eprint/34684/ http://eprints.utm.my/id/eprint/34684/1/KosarAliAmerMFBB2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69727?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QK Botany
spellingShingle	QK Botany Omer, Kosar Ali Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts
description	Ficus deltoidea and Labisia pumila are two local plants that have been used in traditional medicine for along time for enhancement reproductive system and cancer treatment. In this study cell based assay were used to determine the effect of aqueous extract of these two plants on the Human Prostate carcinoma (DU145) cell line. MTT assay, viability test and morphological studies were carried out. Ficus deltoidea showed higher growth inhibitive than Labisia pumila extract. The MTT assay indicated that the most inhibitive concentration of the extracts is 1*10-3 µg/ml, this concentration is consistent through out the course of this study. MTT assay demonstrated that Ficus deltoidea extracts killed around 60% and Labisia pumila around 42% of the cells compared with the negative control. In the viability test Ficus deltoida also recorded a better effect than Labisia pumila which killed all the cells with in 7 days. In the case of Labisia pumila extract is took 9 days, while 12 days needed for negative control. Both extract showed almost similar effect on the morphology of the cells. Observation under inverted microscope for both extracts observed irregular detachment and clumping of the cells. There was morphological changing of the cells from hexagonal to round shape. In apoptotic body study the effect was similar in blabbing cells, chromatin condensation, apoptotic cells and late apoptotic body formation, the difference was in the living cells which for Labisia pumila extract more living cells seen than Ficus deltoidea extract. In general both aqueous extracts showed a growth suppressive effect on the DU145 cell line, but Ficus deltoidea extract was more effective than Labisia pumila extract.
format	Thesis
qualification_level	Master's degree
author	Omer, Kosar Ali
author_facet	Omer, Kosar Ali
author_sort	Omer, Kosar Ali
title	Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts
title_short	Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts
title_full	Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts
title_fullStr	Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts
title_full_unstemmed	Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts
title_sort	survival of human prostate carcinoma cell line (du145) in the presence of ficus deltoidea and labisia pumila aqueouus extracts
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/34684/1/KosarAliAmerMFBB2013.pdf
_version_	1747816234213179392

Survival of human prostate carcinoma cell line (DU145) in the presence of Ficus Deltoidea and Labisia Pumila aqueouus extracts

Similar Items