Identification of locally isolated reactive black 5 decolorizing bacteria

Seven bacterial strains were successfully isolated from textile wastewater. They were screened for their ability to degrade azo dyes including Reactive Black 5 and Acid Orange 7. The screening showed that the decolourization of Reactive Black 5 was best performed under facultative anaerobic conditio...

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Main Author:	Abdullah, Ladei Yaseen
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/35855/5/LadeiYaseenAbdullahMFBB2013.pdf
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spelling	my-utm-ep.358552017-07-24T07:13:29Z Identification of locally isolated reactive black 5 decolorizing bacteria 2013-01 Abdullah, Ladei Yaseen Q Science (General) Seven bacterial strains were successfully isolated from textile wastewater. They were screened for their ability to degrade azo dyes including Reactive Black 5 and Acid Orange 7. The screening showed that the decolourization of Reactive Black 5 was best performed under facultative anaerobic conditions with color removal ranging from 80% - 93% for the three bacterial strains (L1, L6 and L7) at 37°C after 96 hours incubation. However, no decolorization of Acid Orange 7 was observed in all of the isolated bacteria under facultative anaerobic condition. The L1, L6 and L7 were further tested for decolorization of Reactive Black 5 and Acid Orange 7 under aerobic conditions. No decolourization was observed in all sets of experiments. Inducibility studies showed Reactive Black 5 did not show an inducing effect on decolourization process. Two parameters (pH and temperature) were optimized for decolorization. The strains were incubated in medium with pH 4, 7 or 10 and it was found that the optimum pH for decolourization was 7 with 49%, 63% and 70% of decolourization by bacterial strains of L1, L6 and L7, respectively, after 48 hours of incubation. When the strains were incubated at 30°C, 37°C and 55°C, it was found that the optimum temperature was 37°C, which the highest decolourization percentage in the tested strains ranged from 53% - 71% after 48 hours of incubation. The 16S ribosomal ribonucleic acid (16S rRNA) sequences analysis revealed that strains L1 and L6 belonged to genus Bacillus with 99% identity to 16S rRNA gene of Bacillus thuringiensis. Strain L7 was found belong to genus Escherichia with 99% identity to 16S rRNA gene of Escherichia coli strain SCDC-1. 2013-01 Thesis http://eprints.utm.my/id/eprint/35855/ http://eprints.utm.my/id/eprint/35855/5/LadeiYaseenAbdullahMFBB2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Abdullah, Ladei Yaseen Identification of locally isolated reactive black 5 decolorizing bacteria
description	Seven bacterial strains were successfully isolated from textile wastewater. They were screened for their ability to degrade azo dyes including Reactive Black 5 and Acid Orange 7. The screening showed that the decolourization of Reactive Black 5 was best performed under facultative anaerobic conditions with color removal ranging from 80% - 93% for the three bacterial strains (L1, L6 and L7) at 37°C after 96 hours incubation. However, no decolorization of Acid Orange 7 was observed in all of the isolated bacteria under facultative anaerobic condition. The L1, L6 and L7 were further tested for decolorization of Reactive Black 5 and Acid Orange 7 under aerobic conditions. No decolourization was observed in all sets of experiments. Inducibility studies showed Reactive Black 5 did not show an inducing effect on decolourization process. Two parameters (pH and temperature) were optimized for decolorization. The strains were incubated in medium with pH 4, 7 or 10 and it was found that the optimum pH for decolourization was 7 with 49%, 63% and 70% of decolourization by bacterial strains of L1, L6 and L7, respectively, after 48 hours of incubation. When the strains were incubated at 30°C, 37°C and 55°C, it was found that the optimum temperature was 37°C, which the highest decolourization percentage in the tested strains ranged from 53% - 71% after 48 hours of incubation. The 16S ribosomal ribonucleic acid (16S rRNA) sequences analysis revealed that strains L1 and L6 belonged to genus Bacillus with 99% identity to 16S rRNA gene of Bacillus thuringiensis. Strain L7 was found belong to genus Escherichia with 99% identity to 16S rRNA gene of Escherichia coli strain SCDC-1.
format	Thesis
qualification_level	Master's degree
author	Abdullah, Ladei Yaseen
author_facet	Abdullah, Ladei Yaseen
author_sort	Abdullah, Ladei Yaseen
title	Identification of locally isolated reactive black 5 decolorizing bacteria
title_short	Identification of locally isolated reactive black 5 decolorizing bacteria
title_full	Identification of locally isolated reactive black 5 decolorizing bacteria
title_fullStr	Identification of locally isolated reactive black 5 decolorizing bacteria
title_full_unstemmed	Identification of locally isolated reactive black 5 decolorizing bacteria
title_sort	identification of locally isolated reactive black 5 decolorizing bacteria
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/35855/5/LadeiYaseenAbdullahMFBB2013.pdf
_version_	1747816372224655360

Identification of locally isolated reactive black 5 decolorizing bacteria

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