A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia

In the past few decades, lots of studies were carried out to find a way for searching relationships among various races of human beings in understanding their relatedness within a population. This study has been performed to generate DNA sequence data with a view to identifying genetic marker and us...

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Main Author:	Lari, Alireza
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://eprints.utm.my/id/eprint/35897/5/AlirezaLariMFBB2013.pdf
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id	my-utm-ep.35897
record_format	uketd_dc
spelling	my-utm-ep.358972017-09-12T07:44:35Z A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia 2013-01 Lari, Alireza QP Physiology In the past few decades, lots of studies were carried out to find a way for searching relationships among various races of human beings in understanding their relatedness within a population. This study has been performed to generate DNA sequence data with a view to identifying genetic marker and using them in detecting a population in a particular area, such as, in our case, Johor, Malaysia. Mitochondrial DNA (MtDNA) sequence is usually used to detect relatedness between the races within a human population of particular region. The sequence of the region including hypervariable region 1 (HV1, nt16024-16365) and hypervariable region 2 (HV2, nt73-340) are typically used in forensic study. In this study, polymorphisms of (HV1) were investigated to discover a potential forensic marker for detecting different races. In this research PCR amplification after DNA extraction was done to detect the sequence of (HV1). Then, comparisons between Anderson reference - The Cambridge Reference Sequence (rCRS) for human mitochondrial DNA - and the sequences obtained in the current study was done. The results showed the 48 nucleotide variations within the sequence of around 340 bp nucleotides can be used as a genetic marker in forensic study among Malay population of Johor. 2013-01 Thesis http://eprints.utm.my/id/eprint/35897/ http://eprints.utm.my/id/eprint/35897/5/AlirezaLariMFBB2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70730?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Lari, Alireza A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia
description	In the past few decades, lots of studies were carried out to find a way for searching relationships among various races of human beings in understanding their relatedness within a population. This study has been performed to generate DNA sequence data with a view to identifying genetic marker and using them in detecting a population in a particular area, such as, in our case, Johor, Malaysia. Mitochondrial DNA (MtDNA) sequence is usually used to detect relatedness between the races within a human population of particular region. The sequence of the region including hypervariable region 1 (HV1, nt16024-16365) and hypervariable region 2 (HV2, nt73-340) are typically used in forensic study. In this study, polymorphisms of (HV1) were investigated to discover a potential forensic marker for detecting different races. In this research PCR amplification after DNA extraction was done to detect the sequence of (HV1). Then, comparisons between Anderson reference - The Cambridge Reference Sequence (rCRS) for human mitochondrial DNA - and the sequences obtained in the current study was done. The results showed the 48 nucleotide variations within the sequence of around 340 bp nucleotides can be used as a genetic marker in forensic study among Malay population of Johor.
format	Thesis
qualification_level	Master's degree
author	Lari, Alireza
author_facet	Lari, Alireza
author_sort	Lari, Alireza
title	A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia
title_short	A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia
title_full	A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia
title_fullStr	A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia
title_full_unstemmed	A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia
title_sort	study of mt-dna hypervariable region 1 (hvi) sequence polymorphism on residents of johor in malaysia
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/35897/5/AlirezaLariMFBB2013.pdf
_version_	1747816382525865984

A study of mt-dna hypervariable region 1 (HVI) sequence polymorphism on residents of Johor in Malaysia

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