Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)

Hydrogen gas and its usage in electricity generation and transportation is attracting wider interests nowadays. This is because it possesses a high-energy yields (122 kJ g–1), it is a renewable energy source, and does not contribute to the greenhouse effect. In Malaysia, 15.2 million tons of wastewa...

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Main Author:	Ghanbarian, Maryam
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/36549/5/MaryamGhanbarianMFBB2013.pdf
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id	my-utm-ep.36549
record_format	uketd_dc
spelling	my-utm-ep.365492017-09-11T03:34:02Z Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME) 2013-01 Ghanbarian, Maryam Q Science (General) Hydrogen gas and its usage in electricity generation and transportation is attracting wider interests nowadays. This is because it possesses a high-energy yields (122 kJ g–1), it is a renewable energy source, and does not contribute to the greenhouse effect. In Malaysia, 15.2 million tons of wastewater is generated annually by the palm oil industry. The wastewater is known as Palm Oil Mill Effluent (POME). This study is focused on the isolation and characterization of hydrogenproducing bacteria from POME. Samples were taken from the sludge and raw waste of palm oil processing effluent. Bacterial isolation was performed to isolate facultative anaerobes and strict anaerobes. The isolated colonies of bacteria were identified and characterized by microbiological analysis and biochemical tests (catalase, indole, nitrate reduction, urea, TSI and citrate test). From TSI test, it was found that one facultative anaerobe (out of 13 colonies altogether) produced gas; and all strict anaerobes produced gas (8 colonies altogether). The gas-producing colonies were then cultivated at 37°C for 24 hours in a synthetic media simulating starch wastewater for screening of hydrogen production. Hydrogen gas production was then analyzed using RGA (Residual Gas Analyzer). Results show that the facultative anaerobe colony did not produce hydrogen gas by starch fermentation; and four colonies obtained via strict anaerobe isolation show hydrogen production. These colonies can be the subject of future studies to optimize hydrogen production from waste materials. 2013-01 Thesis http://eprints.utm.my/id/eprint/36549/ http://eprints.utm.my/id/eprint/36549/5/MaryamGhanbarianMFBB2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69082?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Ghanbarian, Maryam Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)
description	Hydrogen gas and its usage in electricity generation and transportation is attracting wider interests nowadays. This is because it possesses a high-energy yields (122 kJ g–1), it is a renewable energy source, and does not contribute to the greenhouse effect. In Malaysia, 15.2 million tons of wastewater is generated annually by the palm oil industry. The wastewater is known as Palm Oil Mill Effluent (POME). This study is focused on the isolation and characterization of hydrogenproducing bacteria from POME. Samples were taken from the sludge and raw waste of palm oil processing effluent. Bacterial isolation was performed to isolate facultative anaerobes and strict anaerobes. The isolated colonies of bacteria were identified and characterized by microbiological analysis and biochemical tests (catalase, indole, nitrate reduction, urea, TSI and citrate test). From TSI test, it was found that one facultative anaerobe (out of 13 colonies altogether) produced gas; and all strict anaerobes produced gas (8 colonies altogether). The gas-producing colonies were then cultivated at 37°C for 24 hours in a synthetic media simulating starch wastewater for screening of hydrogen production. Hydrogen gas production was then analyzed using RGA (Residual Gas Analyzer). Results show that the facultative anaerobe colony did not produce hydrogen gas by starch fermentation; and four colonies obtained via strict anaerobe isolation show hydrogen production. These colonies can be the subject of future studies to optimize hydrogen production from waste materials.
format	Thesis
qualification_level	Master's degree
author	Ghanbarian, Maryam
author_facet	Ghanbarian, Maryam
author_sort	Ghanbarian, Maryam
title	Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)
title_short	Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)
title_full	Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)
title_fullStr	Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)
title_full_unstemmed	Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)
title_sort	isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (pome)
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/36549/5/MaryamGhanbarianMFBB2013.pdf
_version_	1747816431354904576

Isolation and characterization of hydrogen producing bacteria from palm oil mill effluent (POME)

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