Degradation of remazol black B by bacillus cereus strain A from textile wastewater

Five bacterial strains designated as A, B1, B2, C and Y1 that were revived from the UTM glycerol stock were used for biodegradation study of azo dyes (SF BLACK EXA, SF RED 3BS, SFN BLUE 150 % and SF YELLOW EXF) at three different conditions; aerobic, facultative anaerobic and partial aerobic (withou...

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Main Author:	Ari, Zainab
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QH Natural history
Online Access:	http://eprints.utm.my/id/eprint/36749/6/ZainabAriMFBSK2013.pdf
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spelling	my-utm-ep.367492017-07-18T06:42:25Z Degradation of remazol black B by bacillus cereus strain A from textile wastewater 2013-05 Ari, Zainab QH Natural history Five bacterial strains designated as A, B1, B2, C and Y1 that were revived from the UTM glycerol stock were used for biodegradation study of azo dyes (SF BLACK EXA, SF RED 3BS, SFN BLUE 150 % and SF YELLOW EXF) at three different conditions; aerobic, facultative anaerobic and partial aerobic (without agitation). Screening test shows bacterium A, under facultative anaerobic condition demonstrated the highest decolourisation rate with removal rate (7.0% h-1) of 0.1 gL-1 SF BLACK EXA was observed. Relationship of growth and decolourization analysis shows decolourisation rate of the same azo dye by bacterium A in CDM medium under facultative anaerobic and aerobic conditions were 26.445% h-1 and 24.56% h-1, respectively. The growth rate of bacterium A was found higher in aerobic culture (2.733 mgh-1) in comparison with that in facultative anaerobic culture (1.067 mgh-1). In other assessment, bacterium A was applied into optimized CDM medium (glucose (1 gL-1), NH4Cl (0.5 gL-1), K2HPO4 (7 gL-1), KH2PO4 (2 gL-1), MgSO4.7H2O (0.1 gL-1), CaCl2 (0.02 gL-1), RBB (0.1 gL-1)), pH 6.5, incubated at 35 °C, with 10 % v/v inoculum under facultative anaerobic condition. Result showed that bacteria A successfully degraded more than 80 % RBB after 20 h incubation. Degradation of RBB molecules increased up to 90 % and 30 % COD removal before attaining stable condition within 164 h incubation. Azoreductase analysis revealed that enzyme was produced intracellularly (11.45 x 10-3 Umin-1) and stabled at pH 6-8 and temperature ranged from 30 ºC to 40 ºC. HPLC analysis revealed that biodegradation of RBB under combined facultative anaerobic-aerobic condition produced sulfanilic acid (Rt = 3.988 min) after 160 h incubation and two unknown metabolites (Rt = 6.071 min and 7.480 min) after 164 h incubations. The partial 16S rRNA analysis showed that bacterium A closely related to Bacillus cereus (99 % sequence similarity). These findings suggest that this bacterium has the capability to assist in the degradation of wastewater containing azo dyes from industry of textile-based. 2013-05 Thesis http://eprints.utm.my/id/eprint/36749/ http://eprints.utm.my/id/eprint/36749/6/ZainabAriMFBSK2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH Natural history
spellingShingle	QH Natural history Ari, Zainab Degradation of remazol black B by bacillus cereus strain A from textile wastewater
description	Five bacterial strains designated as A, B1, B2, C and Y1 that were revived from the UTM glycerol stock were used for biodegradation study of azo dyes (SF BLACK EXA, SF RED 3BS, SFN BLUE 150 % and SF YELLOW EXF) at three different conditions; aerobic, facultative anaerobic and partial aerobic (without agitation). Screening test shows bacterium A, under facultative anaerobic condition demonstrated the highest decolourisation rate with removal rate (7.0% h-1) of 0.1 gL-1 SF BLACK EXA was observed. Relationship of growth and decolourization analysis shows decolourisation rate of the same azo dye by bacterium A in CDM medium under facultative anaerobic and aerobic conditions were 26.445% h-1 and 24.56% h-1, respectively. The growth rate of bacterium A was found higher in aerobic culture (2.733 mgh-1) in comparison with that in facultative anaerobic culture (1.067 mgh-1). In other assessment, bacterium A was applied into optimized CDM medium (glucose (1 gL-1), NH4Cl (0.5 gL-1), K2HPO4 (7 gL-1), KH2PO4 (2 gL-1), MgSO4.7H2O (0.1 gL-1), CaCl2 (0.02 gL-1), RBB (0.1 gL-1)), pH 6.5, incubated at 35 °C, with 10 % v/v inoculum under facultative anaerobic condition. Result showed that bacteria A successfully degraded more than 80 % RBB after 20 h incubation. Degradation of RBB molecules increased up to 90 % and 30 % COD removal before attaining stable condition within 164 h incubation. Azoreductase analysis revealed that enzyme was produced intracellularly (11.45 x 10-3 Umin-1) and stabled at pH 6-8 and temperature ranged from 30 ºC to 40 ºC. HPLC analysis revealed that biodegradation of RBB under combined facultative anaerobic-aerobic condition produced sulfanilic acid (Rt = 3.988 min) after 160 h incubation and two unknown metabolites (Rt = 6.071 min and 7.480 min) after 164 h incubations. The partial 16S rRNA analysis showed that bacterium A closely related to Bacillus cereus (99 % sequence similarity). These findings suggest that this bacterium has the capability to assist in the degradation of wastewater containing azo dyes from industry of textile-based.
format	Thesis
qualification_level	Master's degree
author	Ari, Zainab
author_facet	Ari, Zainab
author_sort	Ari, Zainab
title	Degradation of remazol black B by bacillus cereus strain A from textile wastewater
title_short	Degradation of remazol black B by bacillus cereus strain A from textile wastewater
title_full	Degradation of remazol black B by bacillus cereus strain A from textile wastewater
title_fullStr	Degradation of remazol black B by bacillus cereus strain A from textile wastewater
title_full_unstemmed	Degradation of remazol black B by bacillus cereus strain A from textile wastewater
title_sort	degradation of remazol black b by bacillus cereus strain a from textile wastewater
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/36749/6/ZainabAriMFBSK2013.pdf
_version_	1747816453877268480

Degradation of remazol black B by bacillus cereus strain A from textile wastewater

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