Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR

Flavonol synthase (FLS) converts dihydroflavonol (dihydroquercetin and dihydrokaempferol) to flavonol (quercetin and kaempferol) in flavonoid biosynthetic pathway. Leaves and flower extracts of Melastoma decemfidum are rich in flavonoids and possess strong antioxidant and antitumor activities. In th...

Full description

Saved in:

Bibliographic Details
Main Author:	Norouzi, Azimeh
Format:	Thesis
Published:	2013
Subjects:	TP Chemical technology
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.36876
record_format	uketd_dc
spelling	my-utm-ep.368762020-02-23T06:04:33Z Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR 2013 Norouzi, Azimeh TP Chemical technology Flavonol synthase (FLS) converts dihydroflavonol (dihydroquercetin and dihydrokaempferol) to flavonol (quercetin and kaempferol) in flavonoid biosynthetic pathway. Leaves and flower extracts of Melastoma decemfidum are rich in flavonoids and possess strong antioxidant and antitumor activities. In this study, FLS gene from Melastoma decemfidum was isolated using PCR method. Genomic DNA was extracted from leaf and stem using CTAB method and FLS gene was amplified through degenerate primers using PCR method. Result showed that the size of genomic DNA was =10kb and the A260/A230 and A260/A280 ratios were ranged from 2.03 to 2.13 and 1.85 to 1.97, respectively. The highest DNA yield was obtained from leaf extracts with 437 ng/µl. Three PCR product were amplified with sizes of approximately 200bp, 1500bp and 900bp, at optimal PCR condition. The average full-length of FLS cDNA sequence in different species is ranged from 1 t0 1.4kb. A multicopy gene in plants encodes FLS, and the different copies are expressed in either an organ- or species-specific pattern. Future study such as isolation and verification of FLS gene is required. Then, characterization will be needed in order to elucidate the function of the flavonol synthase that catalyzes the biosynthesis of flavonols in M. decemfidum. 2013 Thesis http://eprints.utm.my/id/eprint/36876/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:76137 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
topic	TP Chemical technology
spellingShingle	TP Chemical technology Norouzi, Azimeh Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR
description	Flavonol synthase (FLS) converts dihydroflavonol (dihydroquercetin and dihydrokaempferol) to flavonol (quercetin and kaempferol) in flavonoid biosynthetic pathway. Leaves and flower extracts of Melastoma decemfidum are rich in flavonoids and possess strong antioxidant and antitumor activities. In this study, FLS gene from Melastoma decemfidum was isolated using PCR method. Genomic DNA was extracted from leaf and stem using CTAB method and FLS gene was amplified through degenerate primers using PCR method. Result showed that the size of genomic DNA was =10kb and the A260/A230 and A260/A280 ratios were ranged from 2.03 to 2.13 and 1.85 to 1.97, respectively. The highest DNA yield was obtained from leaf extracts with 437 ng/µl. Three PCR product were amplified with sizes of approximately 200bp, 1500bp and 900bp, at optimal PCR condition. The average full-length of FLS cDNA sequence in different species is ranged from 1 t0 1.4kb. A multicopy gene in plants encodes FLS, and the different copies are expressed in either an organ- or species-specific pattern. Future study such as isolation and verification of FLS gene is required. Then, characterization will be needed in order to elucidate the function of the flavonol synthase that catalyzes the biosynthesis of flavonols in M. decemfidum.
format	Thesis
qualification_level	Master's degree
author	Norouzi, Azimeh
author_facet	Norouzi, Azimeh
author_sort	Norouzi, Azimeh
title	Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR
title_short	Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR
title_full	Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR
title_fullStr	Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR
title_full_unstemmed	Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR
title_sort	optimization of flavonol synthase (fls) gene amplification from melastoma decemfidum via pcr
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
_version_	1747816471693623296

Optimization of Flavonol Synthase (FLS) gene amplification from Melastoma decemfidum via PCR

Similar Items