Molecular characterization of Malaysian rice germplasm

Rice is one of the most basic and staple food around the world and also is one of the best plants for the study of genetic diversity and genome structure. With the study of the structural and functional features of rice germplasm, the visualization and knowledge about its genetic incomes can be achi...

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Main Author:	Derakhshandeh, Bahareh
Format:	Thesis
Published:	2013
Subjects:	QK Botany
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id	my-utm-ep.37995
record_format	uketd_dc
spelling	my-utm-ep.379952018-06-26T07:52:38Z Molecular characterization of Malaysian rice germplasm 2013-08 Derakhshandeh, Bahareh QK Botany Rice is one of the most basic and staple food around the world and also is one of the best plants for the study of genetic diversity and genome structure. With the study of the structural and functional features of rice germplasm, the visualization and knowledge about its genetic incomes can be achieved, for the reason that it is the carrier of all the inherited characteristics of rice species. Varieties are generally recognized and identified based on morpho-biochemical traits. Most of the traits are quantitative and therefore it is often difficult to use their criteria. Molecular characterization of these varieties would be the ultimate solution. This study focused on identification of Malaysian rice based on their molecular approach. To screen Malaysian rice, 20 varieties among 38 were selected based on the results of qualification and quantification of genomic DNA extraction using CTAB method. Then PCR was carried out with 10 primers and the results of their electrophoresis were analyzed to identify molecular characteristics. These results identified couple of rice varieties. Varieties Malinja, Bahasia and MR263, were identified by the primer RM404, variety MR253 was identified by the primer RM231 and also variety MR263 was defined by the primer RM262. Therefore, this analysis can be expected to greatly increase the efficiency of variety fingerprinting and identification of Malaysian rice cultivars. 2013-08 Thesis http://eprints.utm.my/id/eprint/37995/ masters Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering Faculty of Biosciences and Bioengineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
topic	QK Botany
spellingShingle	QK Botany Derakhshandeh, Bahareh Molecular characterization of Malaysian rice germplasm
description	Rice is one of the most basic and staple food around the world and also is one of the best plants for the study of genetic diversity and genome structure. With the study of the structural and functional features of rice germplasm, the visualization and knowledge about its genetic incomes can be achieved, for the reason that it is the carrier of all the inherited characteristics of rice species. Varieties are generally recognized and identified based on morpho-biochemical traits. Most of the traits are quantitative and therefore it is often difficult to use their criteria. Molecular characterization of these varieties would be the ultimate solution. This study focused on identification of Malaysian rice based on their molecular approach. To screen Malaysian rice, 20 varieties among 38 were selected based on the results of qualification and quantification of genomic DNA extraction using CTAB method. Then PCR was carried out with 10 primers and the results of their electrophoresis were analyzed to identify molecular characteristics. These results identified couple of rice varieties. Varieties Malinja, Bahasia and MR263, were identified by the primer RM404, variety MR253 was identified by the primer RM231 and also variety MR263 was defined by the primer RM262. Therefore, this analysis can be expected to greatly increase the efficiency of variety fingerprinting and identification of Malaysian rice cultivars.
format	Thesis
qualification_level	Master's degree
author	Derakhshandeh, Bahareh
author_facet	Derakhshandeh, Bahareh
author_sort	Derakhshandeh, Bahareh
title	Molecular characterization of Malaysian rice germplasm
title_short	Molecular characterization of Malaysian rice germplasm
title_full	Molecular characterization of Malaysian rice germplasm
title_fullStr	Molecular characterization of Malaysian rice germplasm
title_full_unstemmed	Molecular characterization of Malaysian rice germplasm
title_sort	molecular characterization of malaysian rice germplasm
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering
granting_department	Faculty of Biosciences and Bioengineering
publishDate	2013
_version_	1747816519890370560

Molecular characterization of Malaysian rice germplasm

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