Screening and characterization of thermophiles from Malacca hot spring

Seven strains of thermophilic bacteria (GD-1, GD-2, GD-3, GD-4, GD-5, GD-6 and GD-7) and three strains of thermophilic bacteria (JS-1, JS-2 and JS-3) were isolated and cultivated from samples collected from Kg Ganun Gadek, Alor Gajah and Kg Air Panas, Bemban hot springs respectively which both locat...

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Main Author:	Hong, Li Lian
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QR Microbiology
Online Access:	http://eprints.utm.my/id/eprint/38021/1/HonLiLianMFBSK2013.pdf
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id	my-utm-ep.38021
record_format	uketd_dc
spelling	my-utm-ep.380212018-04-12T05:41:17Z Screening and characterization of thermophiles from Malacca hot spring 2013-03 Hong, Li Lian QR Microbiology Seven strains of thermophilic bacteria (GD-1, GD-2, GD-3, GD-4, GD-5, GD-6 and GD-7) and three strains of thermophilic bacteria (JS-1, JS-2 and JS-3) were isolated and cultivated from samples collected from Kg Ganun Gadek, Alor Gajah and Kg Air Panas, Bemban hot springs respectively which both located at Malacca. The temperature and pH of the Kg Ganun Gadek sampling sites were 55°C and 7.59 respectively while 52 °C and 7.7 for Kg Air Panas Sampling sites. Thermus medium was found to be the most suitable medium for the growth for the eight strains. Strains GD-1, GD-2, GD-3 and GD 5 formed yellow colonies while strains GD-4, GD-6, JS-1, JS-2 and JS-3 formed white colonies. Further studies were done for strains GD-1, GD-2, GD-3, GD-4, GD-5, JS-1, JS-2 and JS-3. Cells of these strains were rod-shaped and occurred in single and chain like arrangement. Only two strains were Gram-negative (JS-2 and JS-3). Strains GD-1, GD-2 and GD-6 were able to hydrolyse starch. Catalase test revealed that strains GD-1, GD-2, GD-3, GD-6, JS-1 and JS-2 able to degraded hydrogen peroxide (H2O2) to water and oxygen. Blast search results of partial 16S rRNA sequences revealed strains GD-1, GD-2 and GD-6 to be Anoxybacillus gonesis while strains GD-3 and GD-5 are Anoxybacillus sp. Strain GD-4 showed closest to Bacillus licheniformis. Strain JS-1 showed closest relationship to Bacillus sp. and Geobacillus kautophilus while the result showed strain JS-3 is the most similar to Bacillus licheniformis and Bacillus sp.. All result has 99% identity. The tentative identities of the strain were supported by the phenotypic and phylogenetic evidence. 2013-03 Thesis http://eprints.utm.my/id/eprint/38021/ http://eprints.utm.my/id/eprint/38021/1/HonLiLianMFBSK2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Hong, Li Lian Screening and characterization of thermophiles from Malacca hot spring
description	Seven strains of thermophilic bacteria (GD-1, GD-2, GD-3, GD-4, GD-5, GD-6 and GD-7) and three strains of thermophilic bacteria (JS-1, JS-2 and JS-3) were isolated and cultivated from samples collected from Kg Ganun Gadek, Alor Gajah and Kg Air Panas, Bemban hot springs respectively which both located at Malacca. The temperature and pH of the Kg Ganun Gadek sampling sites were 55°C and 7.59 respectively while 52 °C and 7.7 for Kg Air Panas Sampling sites. Thermus medium was found to be the most suitable medium for the growth for the eight strains. Strains GD-1, GD-2, GD-3 and GD 5 formed yellow colonies while strains GD-4, GD-6, JS-1, JS-2 and JS-3 formed white colonies. Further studies were done for strains GD-1, GD-2, GD-3, GD-4, GD-5, JS-1, JS-2 and JS-3. Cells of these strains were rod-shaped and occurred in single and chain like arrangement. Only two strains were Gram-negative (JS-2 and JS-3). Strains GD-1, GD-2 and GD-6 were able to hydrolyse starch. Catalase test revealed that strains GD-1, GD-2, GD-3, GD-6, JS-1 and JS-2 able to degraded hydrogen peroxide (H2O2) to water and oxygen. Blast search results of partial 16S rRNA sequences revealed strains GD-1, GD-2 and GD-6 to be Anoxybacillus gonesis while strains GD-3 and GD-5 are Anoxybacillus sp. Strain GD-4 showed closest to Bacillus licheniformis. Strain JS-1 showed closest relationship to Bacillus sp. and Geobacillus kautophilus while the result showed strain JS-3 is the most similar to Bacillus licheniformis and Bacillus sp.. All result has 99% identity. The tentative identities of the strain were supported by the phenotypic and phylogenetic evidence.
format	Thesis
qualification_level	Master's degree
author	Hong, Li Lian
author_facet	Hong, Li Lian
author_sort	Hong, Li Lian
title	Screening and characterization of thermophiles from Malacca hot spring
title_short	Screening and characterization of thermophiles from Malacca hot spring
title_full	Screening and characterization of thermophiles from Malacca hot spring
title_fullStr	Screening and characterization of thermophiles from Malacca hot spring
title_full_unstemmed	Screening and characterization of thermophiles from Malacca hot spring
title_sort	screening and characterization of thermophiles from malacca hot spring
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/38021/1/HonLiLianMFBSK2013.pdf
_version_	1747816522106011648

Screening and characterization of thermophiles from Malacca hot spring

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