Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs

Air-dried and particle form of stems and leaves of Vitis vinifera cultivated in Perlis, Malaysia were extracted using cold, back, methanolic and ethanolic extraction methods. The ethyl acetate stems extract by back extraction (STBE EA) possessed the highest total phenolic content (916.27 ± 0.01) by...

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Main Author:	Zakaria, Nursyahda
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RM Therapeutics Pharmacology
Online Access:	http://eprints.utm.my/id/eprint/38187/1/NursyahdaZakariaMFBB2013.pdf
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id	my-utm-ep.38187
record_format	uketd_dc
spelling	my-utm-ep.381872018-04-12T05:42:30Z Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs 2013-09 Zakaria, Nursyahda RM Therapeutics. Pharmacology Air-dried and particle form of stems and leaves of Vitis vinifera cultivated in Perlis, Malaysia were extracted using cold, back, methanolic and ethanolic extraction methods. The ethyl acetate stems extract by back extraction (STBE EA) possessed the highest total phenolic content (916.27 ± 0.01) by Folin-Ciocalteu assay and radical scavenging activity at IC50 = 18 µg/mL by DPPH assay. The extracts was further investigated by thin layer chromatography resulted in detection of resveratrol at Rf = 0.425 in STBE EA using petroleum ether:ethyl acetate at ratio 2:3. Advanced screening method of phenolic profiling by LC-MS/MS has detected seven phenolic compounds which were identified through LC-MS/MS library and Mass Bank Software. The compounds are trans-resveratrol, trans-cinnamic acid, e-viniferin, salicylic and caffiec acid, catechin, and epigallocatechin. Purification of the STBE EA has resulted in the isolation of trans-stilbene which was identified as 3, 4’, 5- trihydroxystilbene (trans-resveratrol) by Nuclear Magnetic Resonance. The STBE EA and trans-resveratrol exhibited protective effects on HUVECs against oxidative cell damage by hydrogen peroxide (IC50 = 205 µM) with survival rate 17.69% (IC50 = 1 µg/mL) and 40.80% (IC50 = 0.01 µM) and induced nitric oxide 3.41 (IC50 = 0.001 µg/mL) and 3.96 mm (IC50 = 0.001 µM) respectively. STBE EA and trans-resveratrol possessed dual inhibition of COX-2 and 15-LOX 85.07 ± 1.20 (COX-2) and 60% with IC50 at 7.5 µM (15- LOX) respectively. Furthermore purified trans-resveratrol exhibited better inhibition at 96.87 ± 0.10% for COX-2 and 79.57% for 15-LOX. This study demonstrated that STBE EA extracts and trans-resveratrol isolated from Vitis vinifera stems posses potent anti-oxidant and anti-inflammatory properties. 2013-09 Thesis http://eprints.utm.my/id/eprint/38187/ http://eprints.utm.my/id/eprint/38187/1/NursyahdaZakariaMFBB2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	RM Therapeutics Pharmacology
spellingShingle	RM Therapeutics Pharmacology Zakaria, Nursyahda Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs
description	Air-dried and particle form of stems and leaves of Vitis vinifera cultivated in Perlis, Malaysia were extracted using cold, back, methanolic and ethanolic extraction methods. The ethyl acetate stems extract by back extraction (STBE EA) possessed the highest total phenolic content (916.27 ± 0.01) by Folin-Ciocalteu assay and radical scavenging activity at IC50 = 18 µg/mL by DPPH assay. The extracts was further investigated by thin layer chromatography resulted in detection of resveratrol at Rf = 0.425 in STBE EA using petroleum ether:ethyl acetate at ratio 2:3. Advanced screening method of phenolic profiling by LC-MS/MS has detected seven phenolic compounds which were identified through LC-MS/MS library and Mass Bank Software. The compounds are trans-resveratrol, trans-cinnamic acid, e-viniferin, salicylic and caffiec acid, catechin, and epigallocatechin. Purification of the STBE EA has resulted in the isolation of trans-stilbene which was identified as 3, 4’, 5- trihydroxystilbene (trans-resveratrol) by Nuclear Magnetic Resonance. The STBE EA and trans-resveratrol exhibited protective effects on HUVECs against oxidative cell damage by hydrogen peroxide (IC50 = 205 µM) with survival rate 17.69% (IC50 = 1 µg/mL) and 40.80% (IC50 = 0.01 µM) and induced nitric oxide 3.41 (IC50 = 0.001 µg/mL) and 3.96 mm (IC50 = 0.001 µM) respectively. STBE EA and trans-resveratrol possessed dual inhibition of COX-2 and 15-LOX 85.07 ± 1.20 (COX-2) and 60% with IC50 at 7.5 µM (15- LOX) respectively. Furthermore purified trans-resveratrol exhibited better inhibition at 96.87 ± 0.10% for COX-2 and 79.57% for 15-LOX. This study demonstrated that STBE EA extracts and trans-resveratrol isolated from Vitis vinifera stems posses potent anti-oxidant and anti-inflammatory properties.
format	Thesis
qualification_level	Master's degree
author	Zakaria, Nursyahda
author_facet	Zakaria, Nursyahda
author_sort	Zakaria, Nursyahda
title	Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs
title_short	Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs
title_full	Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs
title_fullStr	Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs
title_full_unstemmed	Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs
title_sort	anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of perlis vitis vinifera on cultured huvecs
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/38187/1/NursyahdaZakariaMFBB2013.pdf
_version_	1747816525773930496

Anti inflammatory and anti oxidant properties of purified stilbene and crude extracts of Perlis vitis vinifera on cultured huvecs

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