Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles

Sample preparation is the most time consuming part of elemental analysis where homogeneous samples that are almost free from organic matter is necessary. Therefore, preliminary operations such as separation of interferents and preconcentration of the analytes are required prior to analysis. Ultrason...

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Main Author:	Ahmad, Norazah
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/38862/5/NorazahAhmadMFS2013.pdf
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spelling	my-utm-ep.388622017-07-25T04:42:02Z Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles 2013-01 Ahmad, Norazah QD Chemistry Sample preparation is the most time consuming part of elemental analysis where homogeneous samples that are almost free from organic matter is necessary. Therefore, preliminary operations such as separation of interferents and preconcentration of the analytes are required prior to analysis. Ultrasonic digestion recently proposed as an efficient method for extraction of trace metals from solid samples. The ultrasonic digestion was optimized by using two parameters whilst another two other parameters were used in enzymatic digestion. Conventional one-variable-at-a-time (OVAT) optimization method was applied to both digestion techniques. Fe and Zn were extracted from the samples and were determined by using flame atomic absorption spectrometry (FAAS).The combination of ultrasonic and enzymatic digestion techniques is known as Ultrasonic-Assisted Enzymatic Digestion (USAED) method. The ultrasonication equipment used was ultrasonic bath. Four parameters studied for optimization were sample mass, sonication time, protease enzyme volume and incubation time. The optimum condition of parameters for sample mass was 0.2 g, sonication time of 30 min whilst for enzyme volume was 1000 µL and for incubation time of 24 hours. For the kinetic study, these reactions had undergone zero order reaction. This USAED method was successfully applied for the digestion and kinetic study of the cockle samples. 2013-01 Thesis http://eprints.utm.my/id/eprint/38862/ http://eprints.utm.my/id/eprint/38862/5/NorazahAhmadMFS2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Ahmad, Norazah Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
description	Sample preparation is the most time consuming part of elemental analysis where homogeneous samples that are almost free from organic matter is necessary. Therefore, preliminary operations such as separation of interferents and preconcentration of the analytes are required prior to analysis. Ultrasonic digestion recently proposed as an efficient method for extraction of trace metals from solid samples. The ultrasonic digestion was optimized by using two parameters whilst another two other parameters were used in enzymatic digestion. Conventional one-variable-at-a-time (OVAT) optimization method was applied to both digestion techniques. Fe and Zn were extracted from the samples and were determined by using flame atomic absorption spectrometry (FAAS).The combination of ultrasonic and enzymatic digestion techniques is known as Ultrasonic-Assisted Enzymatic Digestion (USAED) method. The ultrasonication equipment used was ultrasonic bath. Four parameters studied for optimization were sample mass, sonication time, protease enzyme volume and incubation time. The optimum condition of parameters for sample mass was 0.2 g, sonication time of 30 min whilst for enzyme volume was 1000 µL and for incubation time of 24 hours. For the kinetic study, these reactions had undergone zero order reaction. This USAED method was successfully applied for the digestion and kinetic study of the cockle samples.
format	Thesis
qualification_level	Master's degree
author	Ahmad, Norazah
author_facet	Ahmad, Norazah
author_sort	Ahmad, Norazah
title	Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_short	Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_full	Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_fullStr	Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_full_unstemmed	Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
title_sort	application of ultrasonic-assisted enzymatic digestion system for digestion of cockles
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2013
url	http://eprints.utm.my/id/eprint/38862/5/NorazahAhmadMFS2013.pdf
_version_	1747816531665879040

Application of ultrasonic-assisted enzymatic digestion system for digestion of cockles

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