Chemical constituents and bioactivity studies on Premna integrifolia Linn

Premna integrifolia known as buas-buas in Malaysia is one of the species of Verbenaceae family. Extraction, fractionation followed by purification of P. integrifolia afforded seven compounds which consisted of flavone, quinone, ester, plant sterols and diterpene. The structures were elucidated using...

Full description

Saved in:

Bibliographic Details
Main Author:	Nordin, Kamarusalihin
Format:	Thesis
Published:	2013
Subjects:	QK Botany
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.41803
record_format	uketd_dc
spelling	my-utm-ep.418032020-07-05T03:34:40Z Chemical constituents and bioactivity studies on Premna integrifolia Linn 2013 Nordin, Kamarusalihin QK Botany Premna integrifolia known as buas-buas in Malaysia is one of the species of Verbenaceae family. Extraction, fractionation followed by purification of P. integrifolia afforded seven compounds which consisted of flavone, quinone, ester, plant sterols and diterpene. The structures were elucidated using spectroscopic methods including nuclear magnetic resonance (NMR), mass spectrometry (MS), infrared (IR), and ultraviolet (UV) spectroscopies. Two flavones identified as 5,7-dihydroxy-4'-methoxyflavone and 4',5,7-trihydroxyflavone were isolated from the leaves, while two new compounds named integrifolinone and icetexane-X, together with a mixture of two plant sterols, stigmasta-5,22-dien-3ß-ol and stigmasta-5-en-3ß-ol were obtained from the roots. In addition, an ester 2-(4'-hydroxyphenyl)ethyl dodecanoate was isolated from the barks. Antioxidant screening was carried out on the crude extracts and pure compounds. The ethyl acetate extract of the leaves and hexane extract of the roots were found to have low antioxidant activity, while the pure compound icetexane-X gave the most active radical scavenger, with an SC50 of 30.79 µg/mL compared to vitamin C which showed an SC50 of 12.71 µg/mL. In the antimicrobial assay, all the crude extracts and pure compounds were found to be inactive towards all the tested bacteria (Staphylococus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa) 2013 Thesis http://eprints.utm.my/id/eprint/41803/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:81867?queryType=vitalDismax&query=Chemical+constituents+and+bioactivity+studies+on+Premna+&public=true masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
topic	QK Botany
spellingShingle	QK Botany Nordin, Kamarusalihin Chemical constituents and bioactivity studies on Premna integrifolia Linn
description	Premna integrifolia known as buas-buas in Malaysia is one of the species of Verbenaceae family. Extraction, fractionation followed by purification of P. integrifolia afforded seven compounds which consisted of flavone, quinone, ester, plant sterols and diterpene. The structures were elucidated using spectroscopic methods including nuclear magnetic resonance (NMR), mass spectrometry (MS), infrared (IR), and ultraviolet (UV) spectroscopies. Two flavones identified as 5,7-dihydroxy-4'-methoxyflavone and 4',5,7-trihydroxyflavone were isolated from the leaves, while two new compounds named integrifolinone and icetexane-X, together with a mixture of two plant sterols, stigmasta-5,22-dien-3ß-ol and stigmasta-5-en-3ß-ol were obtained from the roots. In addition, an ester 2-(4'-hydroxyphenyl)ethyl dodecanoate was isolated from the barks. Antioxidant screening was carried out on the crude extracts and pure compounds. The ethyl acetate extract of the leaves and hexane extract of the roots were found to have low antioxidant activity, while the pure compound icetexane-X gave the most active radical scavenger, with an SC50 of 30.79 µg/mL compared to vitamin C which showed an SC50 of 12.71 µg/mL. In the antimicrobial assay, all the crude extracts and pure compounds were found to be inactive towards all the tested bacteria (Staphylococus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa)
format	Thesis
qualification_level	Master's degree
author	Nordin, Kamarusalihin
author_facet	Nordin, Kamarusalihin
author_sort	Nordin, Kamarusalihin
title	Chemical constituents and bioactivity studies on Premna integrifolia Linn
title_short	Chemical constituents and bioactivity studies on Premna integrifolia Linn
title_full	Chemical constituents and bioactivity studies on Premna integrifolia Linn
title_fullStr	Chemical constituents and bioactivity studies on Premna integrifolia Linn
title_full_unstemmed	Chemical constituents and bioactivity studies on Premna integrifolia Linn
title_sort	chemical constituents and bioactivity studies on premna integrifolia linn
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2013
_version_	1747816621450199040

Chemical constituents and bioactivity studies on Premna integrifolia Linn

Similar Items