Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater

Biological treatment of textile wastewater using granules involves the application of self-immobilisation of high density biomass under intermittent facultative anaerobic and aerobic system. Since granules are dense and have high settling velocity, high density of active biomass to be retained witho...

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Main Author:	Kee, Thuan Chien
Format:	Thesis
Language:	English
Published:	2012
Subjects:	TD Environmental technology Sanitary engineering
Online Access:	http://eprints.utm.my/id/eprint/41818/1/KeeThuanChienMFBB2012.pdf
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id	my-utm-ep.41818
record_format	uketd_dc
spelling	my-utm-ep.418182017-09-21T04:19:33Z Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater 2012-11 Kee, Thuan Chien TD Environmental technology. Sanitary engineering Biological treatment of textile wastewater using granules involves the application of self-immobilisation of high density biomass under intermittent facultative anaerobic and aerobic system. Since granules are dense and have high settling velocity, high density of active biomass to be retained without being washed out, minimising previous problems of using suspended biomass in treating textile wastewater. The use of synthetic wastewater containing single or several combinations of dyes for the development of granules has been widely studied. However, little has been reported on the development of granules using more complex and toxic real textile wastewater. Hence, there is a need to develop granules that are well adapted to real textile wastewater in order to improve the treatment efficiency. In this study, granules consisting of bio-augmented consortium of four locally isolated decolourising bacteria were successfully developed under intermittent facultative anaerobic-aerobic system. Sludge was added as seeding agent in a single 1 L SBR reactor at hydraulic retention time (HRT) of 6 h. The 16S rDNA molecular analysis showed that ZK1 (JQ773350), ZK2 (JQ773351), ZB1 (JF742762) and ZB2 (JF742761) were closely related to Bacillus pumilus, Bacillus cereus, Brevibacillus panacihumi and Lysinibacillus fusiformis respectively. After 112 days, the size of the granules reached 3.3 ± 1 mm and were dark grey in colour, with integrity coefficient of 25 ± 2, settling velocity of 56 ± 5 m h-1 and sludge volume index (SVI) of 35 ± 5.5 mL g-1. Biomass concentration was 13 ± 0.8 g L-1 and 11 ± 0.6 g L-1 for MLSS and MLVSS respectively. In general, the developed granules showed good removal for colour (70 %; initial ADMI values ranging from 500 to 2000) and COD (53 %; initial values ranging from 400 to 1,500 mg L-1) at HRT of 24 h with intermittent facultative anaerobic (18 h) and aerobic (6 h). 2012-11 Thesis http://eprints.utm.my/id/eprint/41818/ http://eprints.utm.my/id/eprint/41818/1/KeeThuanChienMFBB2012.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TD Environmental technology Sanitary engineering
spellingShingle	TD Environmental technology Sanitary engineering Kee, Thuan Chien Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
description	Biological treatment of textile wastewater using granules involves the application of self-immobilisation of high density biomass under intermittent facultative anaerobic and aerobic system. Since granules are dense and have high settling velocity, high density of active biomass to be retained without being washed out, minimising previous problems of using suspended biomass in treating textile wastewater. The use of synthetic wastewater containing single or several combinations of dyes for the development of granules has been widely studied. However, little has been reported on the development of granules using more complex and toxic real textile wastewater. Hence, there is a need to develop granules that are well adapted to real textile wastewater in order to improve the treatment efficiency. In this study, granules consisting of bio-augmented consortium of four locally isolated decolourising bacteria were successfully developed under intermittent facultative anaerobic-aerobic system. Sludge was added as seeding agent in a single 1 L SBR reactor at hydraulic retention time (HRT) of 6 h. The 16S rDNA molecular analysis showed that ZK1 (JQ773350), ZK2 (JQ773351), ZB1 (JF742762) and ZB2 (JF742761) were closely related to Bacillus pumilus, Bacillus cereus, Brevibacillus panacihumi and Lysinibacillus fusiformis respectively. After 112 days, the size of the granules reached 3.3 ± 1 mm and were dark grey in colour, with integrity coefficient of 25 ± 2, settling velocity of 56 ± 5 m h-1 and sludge volume index (SVI) of 35 ± 5.5 mL g-1. Biomass concentration was 13 ± 0.8 g L-1 and 11 ± 0.6 g L-1 for MLSS and MLVSS respectively. In general, the developed granules showed good removal for colour (70 %; initial ADMI values ranging from 500 to 2000) and COD (53 %; initial values ranging from 400 to 1,500 mg L-1) at HRT of 24 h with intermittent facultative anaerobic (18 h) and aerobic (6 h).
format	Thesis
qualification_level	Master's degree
author	Kee, Thuan Chien
author_facet	Kee, Thuan Chien
author_sort	Kee, Thuan Chien
title	Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
title_short	Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
title_full	Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
title_fullStr	Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
title_full_unstemmed	Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
title_sort	development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2012
url	http://eprints.utm.my/id/eprint/41818/1/KeeThuanChienMFBB2012.pdf
_version_	1747816624885334016

Development of biogranules using selected mix culture of decolourising bacteria for treatment of textile wastewater

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