Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes

Multi walled carbon nanotube (MWCNTs) having high surface area was used for the immobilization of lipase from Candida rugosa (CRL) by physical adsorption. The ability of the MWCNT immobilized CRL was compared against the free CRL in the esterification of benzoic acid with eugenol to produce the anti...

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Main Author:	Abdullah Ismail, Shawnm
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TA Engineering (General) Civil engineering (General)
Online Access:	http://eprints.utm.my/id/eprint/42003/1/ShawnmAbdullahIsmailMFS2014.pdf
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spelling	my-utm-ep.420032017-09-06T01:12:51Z Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes 2014-01 Abdullah Ismail, Shawnm TA Engineering (General). Civil engineering (General) Multi walled carbon nanotube (MWCNTs) having high surface area was used for the immobilization of lipase from Candida rugosa (CRL) by physical adsorption. The ability of the MWCNT immobilized CRL was compared against the free CRL in the esterification of benzoic acid with eugenol to produce the antioxidant eugenol benzoate, a potent lipoxygenase inhibitor. In this study, the effect of four parameters; reaction time, temperature, molecular sieves and enzyme loading were evaluated. The maximum yield of ester of reactions catalyzed by immobilized CRL and free lipase were found to be 31.62 and 27.2%, respectively. The study revealed that the optimal conditions for the highest yield of ester attained for both free CRL and immobilized CRL were similar. The attained optimum conditions were 5 mg/mL enzyme, 0.5:1.5 molar ratio of benzoic acid/eugenol dissolved in 38.5 mL chloroform, 500 mg of molecular sieves with 50 mL total volume of reaction mixture, stirred under 200 rpm at 40oC for 4 h. It was observed the MWCNT immobilized CRL afforded slightly higher yield of ester in all parameters investigated as compared to the free CRL. The outcome suggests that the MWCNT immobilized CRL could be used to synthesize eugenol benzoate. Furthermore, treatment of the esterification process is now easier due to simpler methods of separation of the end product and the biocatalyst could be recycled. 2014-01 Thesis http://eprints.utm.my/id/eprint/42003/ http://eprints.utm.my/id/eprint/42003/1/ShawnmAbdullahIsmailMFS2014.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TA Engineering (General) Civil engineering (General)
spellingShingle	TA Engineering (General) Civil engineering (General) Abdullah Ismail, Shawnm Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes
description	Multi walled carbon nanotube (MWCNTs) having high surface area was used for the immobilization of lipase from Candida rugosa (CRL) by physical adsorption. The ability of the MWCNT immobilized CRL was compared against the free CRL in the esterification of benzoic acid with eugenol to produce the antioxidant eugenol benzoate, a potent lipoxygenase inhibitor. In this study, the effect of four parameters; reaction time, temperature, molecular sieves and enzyme loading were evaluated. The maximum yield of ester of reactions catalyzed by immobilized CRL and free lipase were found to be 31.62 and 27.2%, respectively. The study revealed that the optimal conditions for the highest yield of ester attained for both free CRL and immobilized CRL were similar. The attained optimum conditions were 5 mg/mL enzyme, 0.5:1.5 molar ratio of benzoic acid/eugenol dissolved in 38.5 mL chloroform, 500 mg of molecular sieves with 50 mL total volume of reaction mixture, stirred under 200 rpm at 40oC for 4 h. It was observed the MWCNT immobilized CRL afforded slightly higher yield of ester in all parameters investigated as compared to the free CRL. The outcome suggests that the MWCNT immobilized CRL could be used to synthesize eugenol benzoate. Furthermore, treatment of the esterification process is now easier due to simpler methods of separation of the end product and the biocatalyst could be recycled.
format	Thesis
qualification_level	Master's degree
author	Abdullah Ismail, Shawnm
author_facet	Abdullah Ismail, Shawnm
author_sort	Abdullah Ismail, Shawnm
title	Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes
title_short	Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes
title_full	Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes
title_fullStr	Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes
title_full_unstemmed	Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes
title_sort	synthesis of the anti-oxidant eugenol benzoate using candida rugosa lipase immobilized onto multi walled carbon nanotubes
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2014
url	http://eprints.utm.my/id/eprint/42003/1/ShawnmAbdullahIsmailMFS2014.pdf
_version_	1747816668186279936

Synthesis of the anti-oxidant eugenol benzoate using Candida rugosa lipase immobilized onto multi walled carbon nanotubes

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