Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.

Chemical investigations of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb. have been carried out. The stems of P. porphyrophyllum and the whole plant of P. sarmentosum, each were extracted by maceration using petroleum ether, dichloromethane, ethyl acetate and methanol consecutively. Pur...

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Main Author:	Sidek, Raihan Murni
Format:	Thesis
Published:	2013
Subjects:	QK Botany
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id	my-utm-ep.42194
record_format	uketd_dc
spelling	my-utm-ep.421942020-08-17T02:26:00Z Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb. 2013 Sidek, Raihan Murni QK Botany Chemical investigations of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb. have been carried out. The stems of P. porphyrophyllum and the whole plant of P. sarmentosum, each were extracted by maceration using petroleum ether, dichloromethane, ethyl acetate and methanol consecutively. Purification of the crude extracts was carried out using several chromatographic techniques. The structures of the pure compounds were elucidated by spectroscopic methods such as IR, 1D (1H, 13C, DEPT) and 2D (COSY, HMQC, HMBC) NMR and GC-MS. Four compounds have been identified from the petroleum ether extract of P. Porphyrophyllum and were characterized as ß-sitosterol, sesamin, N-isobutyl-15- (3’,4’-methylenedioxyphenyl)-2E,4E,12E-pentadecatrienamide and a new compound, 5-hydroxy-7,2’,4’,6’-tetramethoxyflavone. Meanwhile, 5,7,3’,4’,5’- pentamethoxyflavone was identified from the dichloromethane extract. Four compounds were isolated from P. sarmentosum. The petroleum ether extract afforded 3’,4’-methylenedioxyphenyl-13-tetradecene while the dichloromethane extract gave asaricin and 2-(methyl-2’-propenoate)-4,5- methylenedioxybenzaldehyde. Purification of the methanol extract gave 4-(2’- octenyl)phenol. Pure compounds which were isolated abundantly were screened for cytotoxic study using methylthiazol tetrazolium (MTT) cell proliferation assay. 4- (2’-Octenyl)phenol was found to have the highest cytotoxic effect towards the cell with the percentage of cell inhibition of 12.96%. 2013 Thesis http://eprints.utm.my/id/eprint/42194/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:75252 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
topic	QK Botany
spellingShingle	QK Botany Sidek, Raihan Murni Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.
description	Chemical investigations of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb. have been carried out. The stems of P. porphyrophyllum and the whole plant of P. sarmentosum, each were extracted by maceration using petroleum ether, dichloromethane, ethyl acetate and methanol consecutively. Purification of the crude extracts was carried out using several chromatographic techniques. The structures of the pure compounds were elucidated by spectroscopic methods such as IR, 1D (1H, 13C, DEPT) and 2D (COSY, HMQC, HMBC) NMR and GC-MS. Four compounds have been identified from the petroleum ether extract of P. Porphyrophyllum and were characterized as ß-sitosterol, sesamin, N-isobutyl-15- (3’,4’-methylenedioxyphenyl)-2E,4E,12E-pentadecatrienamide and a new compound, 5-hydroxy-7,2’,4’,6’-tetramethoxyflavone. Meanwhile, 5,7,3’,4’,5’- pentamethoxyflavone was identified from the dichloromethane extract. Four compounds were isolated from P. sarmentosum. The petroleum ether extract afforded 3’,4’-methylenedioxyphenyl-13-tetradecene while the dichloromethane extract gave asaricin and 2-(methyl-2’-propenoate)-4,5- methylenedioxybenzaldehyde. Purification of the methanol extract gave 4-(2’- octenyl)phenol. Pure compounds which were isolated abundantly were screened for cytotoxic study using methylthiazol tetrazolium (MTT) cell proliferation assay. 4- (2’-Octenyl)phenol was found to have the highest cytotoxic effect towards the cell with the percentage of cell inhibition of 12.96%.
format	Thesis
qualification_level	Master's degree
author	Sidek, Raihan Murni
author_facet	Sidek, Raihan Murni
author_sort	Sidek, Raihan Murni
title	Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.
title_short	Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.
title_full	Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.
title_fullStr	Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.
title_full_unstemmed	Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.
title_sort	phytochemicals and bioactivity of piper porphyrophyllum n. e. br. and piper sarmentosum roxb.
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2013
_version_	1747816712674213888

Phytochemicals and bioactivity of Piper porphyrophyllum N. E. Br. and Piper sarmentosum Roxb.

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