Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture

The study of fed batch fermentation was carried out to enhance the production of CGTase from Bacillus sp. TS1-1. The microbes was grown in 2 %(w/v) soluble starch, 11%(w/v) yeast extract, 0.1 %(w/v) K2HPO4, 0.02 %(w/v) MgSO4.7H2O and 10 %(w/v) Na2CO3 solution. Batch fermentation was carried out as c...

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Main Author:	Wan Md. Zain, Wan Salwanis
Format:	Thesis
Language:	English
Published:	2005
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/4373/1/WanSalwanisWanMdZainMFChE2005.pdf
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spelling	my-utm-ep.43732018-01-28T03:20:41Z Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture 2005-11 Wan Md. Zain, Wan Salwanis TP Chemical technology The study of fed batch fermentation was carried out to enhance the production of CGTase from Bacillus sp. TS1-1. The microbes was grown in 2 %(w/v) soluble starch, 11%(w/v) yeast extract, 0.1 %(w/v) K2HPO4, 0.02 %(w/v) MgSO4.7H2O and 10 %(w/v) Na2CO3 solution. Batch fermentation was carried out as control using 5 L fermentor with 4 L working volume. A maximum CGTase activity of 70.32 U/ml was observed during the stationary phase of growth with specific activity of 0.198 U/Âµg proteins. The fed batch study was carried out to obtain the best feeding mode, carbon and nitrogen sources. Constant feed rate fed batch result gave the highest increment in CGTase production by 25.3% as compared to the batch fermentation. Tapioca starch at concentration of 2 %(w/v) was selected as the best inducer for both CGTase and biomass production, where improvement of 35.6% and 25.7% was observed respectively, as compared to the batch fermentation. The addition of 0.5 %(w/v) nitrogen source in the feeding medium failed to improve the CGTase production, but on the other hand increased the biomass significantly. An increment of 69.3% in terms of biomass production as opposed to batch fermentation was obtained with yeast extract. The optimization of carbon and nitrogen concentration using tapioca starch and yeast extract was carried out using Response Surface Methodology (RSM). The optimum condition obtained were 3.3 %(w/v) of tapioca starch and 0.13 %(w/v) of yeast extract. The optimized medium improved the CGTase production up to 13.9% as compared to batch fermentation. The production of CGTase in repeated fed batch fermentation using 2 %(w/v) of tapioca starch was quite consistent even after the third addition of fresh medium with maximum activity fluctuating between 80 - 86 U/ml. 2005-11 Thesis http://eprints.utm.my/id/eprint/4373/ http://eprints.utm.my/id/eprint/4373/1/WanSalwanisWanMdZainMFChE2005.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering Faculty of Chemical and Natural Resources Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Wan Md. Zain, Wan Salwanis Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture
description	The study of fed batch fermentation was carried out to enhance the production of CGTase from Bacillus sp. TS1-1. The microbes was grown in 2 %(w/v) soluble starch, 11%(w/v) yeast extract, 0.1 %(w/v) K2HPO4, 0.02 %(w/v) MgSO4.7H2O and 10 %(w/v) Na2CO3 solution. Batch fermentation was carried out as control using 5 L fermentor with 4 L working volume. A maximum CGTase activity of 70.32 U/ml was observed during the stationary phase of growth with specific activity of 0.198 U/Âµg proteins. The fed batch study was carried out to obtain the best feeding mode, carbon and nitrogen sources. Constant feed rate fed batch result gave the highest increment in CGTase production by 25.3% as compared to the batch fermentation. Tapioca starch at concentration of 2 %(w/v) was selected as the best inducer for both CGTase and biomass production, where improvement of 35.6% and 25.7% was observed respectively, as compared to the batch fermentation. The addition of 0.5 %(w/v) nitrogen source in the feeding medium failed to improve the CGTase production, but on the other hand increased the biomass significantly. An increment of 69.3% in terms of biomass production as opposed to batch fermentation was obtained with yeast extract. The optimization of carbon and nitrogen concentration using tapioca starch and yeast extract was carried out using Response Surface Methodology (RSM). The optimum condition obtained were 3.3 %(w/v) of tapioca starch and 0.13 %(w/v) of yeast extract. The optimized medium improved the CGTase production up to 13.9% as compared to batch fermentation. The production of CGTase in repeated fed batch fermentation using 2 %(w/v) of tapioca starch was quite consistent even after the third addition of fresh medium with maximum activity fluctuating between 80 - 86 U/ml.
format	Thesis
qualification_level	Master's degree
author	Wan Md. Zain, Wan Salwanis
author_facet	Wan Md. Zain, Wan Salwanis
author_sort	Wan Md. Zain, Wan Salwanis
title	Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture
title_short	Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture
title_full	Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture
title_fullStr	Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture
title_full_unstemmed	Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture
title_sort	production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. ts1-1 using fed batch culture
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical and Natural Resources Engineering
granting_department	Faculty of Chemical and Natural Resources Engineering
publishDate	2005
url	http://eprints.utm.my/id/eprint/4373/1/WanSalwanisWanMdZainMFChE2005.pdf
_version_	1747814521411469312

Production of cyclodextrin glucanotransferase from alkalophilic bacillus sp. Ts1-1 using fed batch culture

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