The potential use of organic acids in beef preservation industry

Acetic acid and lactic acid can inhibit growth of Gram negative bacteria particularly a pathogenic species. In food industries acetic acid and lactic acid were used as preservative. Current study is to immerse beef carcasses in acetic acid and lactic acid with E. coli strains BL21 (DE3), DH5a and an...

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Main Author: Hama Faraj, Goran Sedeeq
Format: Thesis
Language:English
Published: 2014
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Online Access:http://eprints.utm.my/id/eprint/48012/25/GoranSedeeqHamaMFBME2014.pdf
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spelling my-utm-ep.480122017-08-11T05:55:54Z The potential use of organic acids in beef preservation industry 2014-05 Hama Faraj, Goran Sedeeq R Medicine (General) Acetic acid and lactic acid can inhibit growth of Gram negative bacteria particularly a pathogenic species. In food industries acetic acid and lactic acid were used as preservative. Current study is to immerse beef carcasses in acetic acid and lactic acid with E. coli strains BL21 (DE3), DH5a and an unknown strain. Beef carcass pieces were immersed in the E. coli suspension which contained (107 CFU/ml) for 30 seconds and left for 2 hours to allow E. coli to settle down on the surface of beef carcass. The beef carcass pieces were thereafter immersed in 1.0, 2.0 and 3.0% solutions of acetic acid and 1.5, 2.5 and 3.5% solutions of lactic acid for 30 seconds, then tap water was used to wash the samples. A clean specimen 5 x 5 cm was put on the surface of each sample. The count of E. coli on beef surface immersed in 1.0, 2.0 and 3.0% of acetic acid reduced by 0.87, 1.33 and 1.73 log CFU/ cm2, respectively also reduced by 1.1, 1.9 and 2.47 log CFU/cm2 for 1.5, 2.5 and 3.5% of lactic acid, respectively. The results showed that concentration of acetic acid and lactic acid had a significant effect to reduce E. coli count. The results also proved that lactic acid had greater effect on non-pathogenic E. coli rather than acetic acid. 2014-05 Thesis http://eprints.utm.my/id/eprint/48012/ http://eprints.utm.my/id/eprint/48012/25/GoranSedeeqHamaMFBME2014.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Hama Faraj, Goran Sedeeq
The potential use of organic acids in beef preservation industry
description Acetic acid and lactic acid can inhibit growth of Gram negative bacteria particularly a pathogenic species. In food industries acetic acid and lactic acid were used as preservative. Current study is to immerse beef carcasses in acetic acid and lactic acid with E. coli strains BL21 (DE3), DH5a and an unknown strain. Beef carcass pieces were immersed in the E. coli suspension which contained (107 CFU/ml) for 30 seconds and left for 2 hours to allow E. coli to settle down on the surface of beef carcass. The beef carcass pieces were thereafter immersed in 1.0, 2.0 and 3.0% solutions of acetic acid and 1.5, 2.5 and 3.5% solutions of lactic acid for 30 seconds, then tap water was used to wash the samples. A clean specimen 5 x 5 cm was put on the surface of each sample. The count of E. coli on beef surface immersed in 1.0, 2.0 and 3.0% of acetic acid reduced by 0.87, 1.33 and 1.73 log CFU/ cm2, respectively also reduced by 1.1, 1.9 and 2.47 log CFU/cm2 for 1.5, 2.5 and 3.5% of lactic acid, respectively. The results showed that concentration of acetic acid and lactic acid had a significant effect to reduce E. coli count. The results also proved that lactic acid had greater effect on non-pathogenic E. coli rather than acetic acid.
format Thesis
qualification_level Master's degree
author Hama Faraj, Goran Sedeeq
author_facet Hama Faraj, Goran Sedeeq
author_sort Hama Faraj, Goran Sedeeq
title The potential use of organic acids in beef preservation industry
title_short The potential use of organic acids in beef preservation industry
title_full The potential use of organic acids in beef preservation industry
title_fullStr The potential use of organic acids in beef preservation industry
title_full_unstemmed The potential use of organic acids in beef preservation industry
title_sort potential use of organic acids in beef preservation industry
granting_institution Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department Faculty of Biosciences and Medical Engineering
publishDate 2014
url http://eprints.utm.my/id/eprint/48012/25/GoranSedeeqHamaMFBME2014.pdf
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