Effects of heavy metals and selected carbon compounds on the growth of bacterium CA

Nowadays environment has been suffering from the contamination by heavy metals and organic compounds. Modernization, urbanization and industrialization further increase the levels of contamination. In this work, the ability of bacterium CA previously isolated from textile waste water was investigate...

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Main Author:	Mohammed Saleh, Hakar
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TD Environmental technology Sanitary engineering
Online Access:	http://eprints.utm.my/id/eprint/48419/25/HakarMohammedSalehMFBME2015.pdf
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spelling	my-utm-ep.484192017-08-08T03:31:50Z Effects of heavy metals and selected carbon compounds on the growth of bacterium CA 2014-06 Mohammed Saleh, Hakar TD Environmental technology. Sanitary engineering Nowadays environment has been suffering from the contamination by heavy metals and organic compounds. Modernization, urbanization and industrialization further increase the levels of contamination. In this work, the ability of bacterium CA previously isolated from textile waste water was investigated to tolerate to aluminium, copper, cobalt, zinc and manganese at different concentrations. The MIC were found to be 400, 200, 40, 60, 300 mg/L, respectively, the growth rate at MIC were 0.1732, 0.417, 0.0885, 0.2824 and 0.4046 h-1, respectively, and the doubling time values at MIC were 4.005, 1.66187, 7.83, 2.4539 and 1.7128 h-1, respectively. Different organic compounds were supplied as sole carbon sources at chemically defined media. The bacterium CA could grow on the CDM provided with casamino acids, casamino acids (as both carbon and nitrogen source), glucose and glycerol with growth rate of 0.0297, 0.0317, 0.04 and 0.0492 h-1, respectively. Whereas the bacterium failed to grow when sulfanilic acids, acrylamide and benzene were only source of carbon. Bacterium CA was found to be sensitive to tetracycline hydrochloride and kanamycin sulfate with clear zones of 2.8 and 2.6 cm, respectively, while it was resistant to XY-12 and ampicillin trihydrate. Moreover, bacterium CA was found to be closest with Bacillus thuringiensis with accession number NR_102506 via 16S rRNA gene analysis. In conclusion, bacterium CA was positive to tolerate to heavy metals, results were significant in case of aluminium, manganese and copper. Bacterium CA was able to grow on different organic compounds that indicated the presence of different mechanisms of carbon source up take and metabolism in the bacterium system. The obtained results suggested the bacterium CA could be potentially used for the treatment of wastewater contamination containing heavy metals and organic compounds. 2014-06 Thesis http://eprints.utm.my/id/eprint/48419/ http://eprints.utm.my/id/eprint/48419/25/HakarMohammedSalehMFBME2015.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TD Environmental technology Sanitary engineering
spellingShingle	TD Environmental technology Sanitary engineering Mohammed Saleh, Hakar Effects of heavy metals and selected carbon compounds on the growth of bacterium CA
description	Nowadays environment has been suffering from the contamination by heavy metals and organic compounds. Modernization, urbanization and industrialization further increase the levels of contamination. In this work, the ability of bacterium CA previously isolated from textile waste water was investigated to tolerate to aluminium, copper, cobalt, zinc and manganese at different concentrations. The MIC were found to be 400, 200, 40, 60, 300 mg/L, respectively, the growth rate at MIC were 0.1732, 0.417, 0.0885, 0.2824 and 0.4046 h-1, respectively, and the doubling time values at MIC were 4.005, 1.66187, 7.83, 2.4539 and 1.7128 h-1, respectively. Different organic compounds were supplied as sole carbon sources at chemically defined media. The bacterium CA could grow on the CDM provided with casamino acids, casamino acids (as both carbon and nitrogen source), glucose and glycerol with growth rate of 0.0297, 0.0317, 0.04 and 0.0492 h-1, respectively. Whereas the bacterium failed to grow when sulfanilic acids, acrylamide and benzene were only source of carbon. Bacterium CA was found to be sensitive to tetracycline hydrochloride and kanamycin sulfate with clear zones of 2.8 and 2.6 cm, respectively, while it was resistant to XY-12 and ampicillin trihydrate. Moreover, bacterium CA was found to be closest with Bacillus thuringiensis with accession number NR_102506 via 16S rRNA gene analysis. In conclusion, bacterium CA was positive to tolerate to heavy metals, results were significant in case of aluminium, manganese and copper. Bacterium CA was able to grow on different organic compounds that indicated the presence of different mechanisms of carbon source up take and metabolism in the bacterium system. The obtained results suggested the bacterium CA could be potentially used for the treatment of wastewater contamination containing heavy metals and organic compounds.
format	Thesis
qualification_level	Master's degree
author	Mohammed Saleh, Hakar
author_facet	Mohammed Saleh, Hakar
author_sort	Mohammed Saleh, Hakar
title	Effects of heavy metals and selected carbon compounds on the growth of bacterium CA
title_short	Effects of heavy metals and selected carbon compounds on the growth of bacterium CA
title_full	Effects of heavy metals and selected carbon compounds on the growth of bacterium CA
title_fullStr	Effects of heavy metals and selected carbon compounds on the growth of bacterium CA
title_full_unstemmed	Effects of heavy metals and selected carbon compounds on the growth of bacterium CA
title_sort	effects of heavy metals and selected carbon compounds on the growth of bacterium ca
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/48419/25/HakarMohammedSalehMFBME2015.pdf
_version_	1747817385697476608

Effects of heavy metals and selected carbon compounds on the growth of bacterium CA

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