Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil

Pseudomonas is an aerobic Gram-negative, rod-shape bacterium which emerged as one of the most problematic nosocomial pathogens. To characterize Pseudpmonas species strains that are widespread in animal, plant, human, and environment especially soil and water, the soil sample was collected from T02 B...

Full description

Saved in:

Bibliographic Details
Main Author:	Karim Hameed, Ashty
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QP Physiology
Online Access:	http://eprints.utm.my/id/eprint/48498/1/AshtyKarimHameedMFBME2014.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.48498
record_format	uketd_dc
spelling	my-utm-ep.484982017-07-27T02:37:40Z Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil 2014 Karim Hameed, Ashty QP Physiology Pseudomonas is an aerobic Gram-negative, rod-shape bacterium which emerged as one of the most problematic nosocomial pathogens. To characterize Pseudpmonas species strains that are widespread in animal, plant, human, and environment especially soil and water, the soil sample was collected from T02 Building in Universiti Teknologi Malaysia. An isolated bacterium was cultured on nutrient agar and MacConkey agar plates. The results showed that using (MacConkey agar) at 37°C had highest recovery in the isolation of Pseudomonas. The colony produced white- pink pigment. Based on the Gram-staining method, Pseudomonas species was Gram negative with rod in shape, in pink color. The effects of temperature and incubation time, as well as their growth profiles in nutrient broth media, were studied. Pseudomonas can grow well in 37OC, which recorded maximum growth rate, and at 30OC Pseudomonas grow nearly similar to its growth rate at 37OC, while at 25OC growth of Pseudomonas was slow. Methods for isolation and characterization of Pseudomonas species based upon culture methods coupled with biochemical tests were used in this study. According to biochemical tests, Pseudomonas was catalase positive, citrate positive, gelatin liquefaction and oxidase positive whereas was negative for methyl red, urease, starch hydrolysis, triple sugar iron and Indole. However, to study the susceptibility of tested strain to different antibiotics using disk diffusion method the result showed that the isolated strain of Pseudomonas was resistance to Erythromycin, Gentamycin, Ampicillin and Penicillin, and susceptible to Ciprofloxacin, Imipenin, Lincomycin, Piperacillin and Tobramycin. 2014 Thesis http://eprints.utm.my/id/eprint/48498/ http://eprints.utm.my/id/eprint/48498/1/AshtyKarimHameedMFBME2014.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:79990?queryType=vitalDismax&query=Isolation+and+antibiotic+susceptibility+screening+of+Pseudomonas+Sp.+from+soil&public=true masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Karim Hameed, Ashty Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil
description	Pseudomonas is an aerobic Gram-negative, rod-shape bacterium which emerged as one of the most problematic nosocomial pathogens. To characterize Pseudpmonas species strains that are widespread in animal, plant, human, and environment especially soil and water, the soil sample was collected from T02 Building in Universiti Teknologi Malaysia. An isolated bacterium was cultured on nutrient agar and MacConkey agar plates. The results showed that using (MacConkey agar) at 37°C had highest recovery in the isolation of Pseudomonas. The colony produced white- pink pigment. Based on the Gram-staining method, Pseudomonas species was Gram negative with rod in shape, in pink color. The effects of temperature and incubation time, as well as their growth profiles in nutrient broth media, were studied. Pseudomonas can grow well in 37OC, which recorded maximum growth rate, and at 30OC Pseudomonas grow nearly similar to its growth rate at 37OC, while at 25OC growth of Pseudomonas was slow. Methods for isolation and characterization of Pseudomonas species based upon culture methods coupled with biochemical tests were used in this study. According to biochemical tests, Pseudomonas was catalase positive, citrate positive, gelatin liquefaction and oxidase positive whereas was negative for methyl red, urease, starch hydrolysis, triple sugar iron and Indole. However, to study the susceptibility of tested strain to different antibiotics using disk diffusion method the result showed that the isolated strain of Pseudomonas was resistance to Erythromycin, Gentamycin, Ampicillin and Penicillin, and susceptible to Ciprofloxacin, Imipenin, Lincomycin, Piperacillin and Tobramycin.
format	Thesis
qualification_level	Master's degree
author	Karim Hameed, Ashty
author_facet	Karim Hameed, Ashty
author_sort	Karim Hameed, Ashty
title	Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil
title_short	Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil
title_full	Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil
title_fullStr	Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil
title_full_unstemmed	Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil
title_sort	isolation and antibiotic susceptibility screening of pseudomonas sp. from soil
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/48498/1/AshtyKarimHameedMFBME2014.pdf
_version_	1747817405056286720

Isolation and antibiotic susceptibility screening of Pseudomonas Sp. from soil

Similar Items