Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production

Hydrogen is a clean energy substitute to fossil fuels, also environmentally friendly and does not lead to global warming. Photosynthetic bacteria produce hydrogen from organic compounds by an anaerobic light-dependent electron transfer process. Two hydrogen-producing photosynthetic bacteria have bee...

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Main Author:	Mohammed Khalid, Karzan
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TD Environmental technology Sanitary engineering
Online Access:	http://eprints.utm.my/id/eprint/48500/1/KarzanMohammedKhalidMFBME2014.pdf
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spelling	my-utm-ep.485002017-08-02T04:56:16Z Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production 2014 Mohammed Khalid, Karzan TD Environmental technology. Sanitary engineering Hydrogen is a clean energy substitute to fossil fuels, also environmentally friendly and does not lead to global warming. Photosynthetic bacteria produce hydrogen from organic compounds by an anaerobic light-dependent electron transfer process. Two hydrogen-producing photosynthetic bacteria have been isolated from aquaculture wastewater enriched with succinate (electron donor) under facultative condition. The isolated bacteria were identified as Gram negative and rod shaped bacteria. During the photohydrogen production process, hydrogen gas is produced at 30ºC, initial pH of 7, and light intensity provided using white fluorescent lamp. The two isolated bacteria were then screened for hydrogen production using acetate and starch as substrate. Hydrogen production was measured using residual gas analyzer. These strains have the ability to utilize starch better than acetate as carbon sources for hydrogen production. The two strains, designated as AQ1 and AQ4, were identified to be Pseudomonas sp. via 16S rRNA characterization. The sequences have been deposited to GenBank with the accession number KJ854409 for Pseudomonas sp. AQ1 and KJ854410 for Pseudomonas sp. AQ4. In conclusion, the two isolates (AQ1 and AQ4) have been characterized as hydrogen producer in photohydrogen production process using starch and acetate as substrate. They have the potential to be used in simultaneous wastewater treatment and clean energy generation. 2014 Thesis http://eprints.utm.my/id/eprint/48500/ http://eprints.utm.my/id/eprint/48500/1/KarzanMohammedKhalidMFBME2014.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:83730?queryType=vitalDismax&query=Isolation+and+characterization+of+photosynthetic+bacteria+from+aquaculture+wastewater+for+hydrogen+production&public=true masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TD Environmental technology Sanitary engineering
spellingShingle	TD Environmental technology Sanitary engineering Mohammed Khalid, Karzan Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
description	Hydrogen is a clean energy substitute to fossil fuels, also environmentally friendly and does not lead to global warming. Photosynthetic bacteria produce hydrogen from organic compounds by an anaerobic light-dependent electron transfer process. Two hydrogen-producing photosynthetic bacteria have been isolated from aquaculture wastewater enriched with succinate (electron donor) under facultative condition. The isolated bacteria were identified as Gram negative and rod shaped bacteria. During the photohydrogen production process, hydrogen gas is produced at 30ºC, initial pH of 7, and light intensity provided using white fluorescent lamp. The two isolated bacteria were then screened for hydrogen production using acetate and starch as substrate. Hydrogen production was measured using residual gas analyzer. These strains have the ability to utilize starch better than acetate as carbon sources for hydrogen production. The two strains, designated as AQ1 and AQ4, were identified to be Pseudomonas sp. via 16S rRNA characterization. The sequences have been deposited to GenBank with the accession number KJ854409 for Pseudomonas sp. AQ1 and KJ854410 for Pseudomonas sp. AQ4. In conclusion, the two isolates (AQ1 and AQ4) have been characterized as hydrogen producer in photohydrogen production process using starch and acetate as substrate. They have the potential to be used in simultaneous wastewater treatment and clean energy generation.
format	Thesis
qualification_level	Master's degree
author	Mohammed Khalid, Karzan
author_facet	Mohammed Khalid, Karzan
author_sort	Mohammed Khalid, Karzan
title	Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
title_short	Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
title_full	Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
title_fullStr	Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
title_full_unstemmed	Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
title_sort	isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/48500/1/KarzanMohammedKhalidMFBME2014.pdf
_version_	1747817405548068864

Isolation and characterization of photosynthetic bacteria from aquaculture wastewater for hydrogen production

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