Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition

Ganoderma boninense is a basidiomycetes white rot fungus which causes basal stem rot (BSR), one major disease in oil palm plantation in Malaysia. The objective of the present study was to screen the most potential fungus that has a capability to produce crude xylanase and cellulase to be used as a b...

Full description

Saved in:

Bibliographic Details
Main Author:	Mohd. Noor, Rohaya
Format:	Thesis
Language:	English
Published:	2014
Subjects:	QK Botany
Online Access:	http://eprints.utm.my/id/eprint/48605/1/RohayaMohdNoorMFBME2014.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.48605
record_format	uketd_dc
spelling	my-utm-ep.486052020-02-06T06:47:23Z Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition 2014 Mohd. Noor, Rohaya QK Botany Ganoderma boninense is a basidiomycetes white rot fungus which causes basal stem rot (BSR), one major disease in oil palm plantation in Malaysia. The objective of the present study was to screen the most potential fungus that has a capability to produce crude xylanase and cellulase to be used as a biological control for Ganoderma boninense. Twenty four different strains of fungi were obtained from the Biorefinery Technology Research Laboratory of Faculty Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor. They were screened for their potential to degrade cellulose and hemicelluloses. The screening of the fungi were based on the diameter of holozones on red color of congo red and the formation of yellow-opaque area on the carboxymethlycellulose(CMC) and xylan agar, respectively. Among these strains, five potential fungi which showed the maximum enzyme activities for endoglucanase (CMCase), filter paper degrading enzyme (FPase), beta-glucosidase, xylanase and reducing sugar were selected to produce enzyme cocktail on untreated oil palm trunk will be further study through its performance in solid state fermentation. The potential enzyme producers from fungi were CT2, Pycnoporus cinnabarinus, EFB1, EFB2 and TG6 that produced 304.04 U/g of CMCase, 13.25 U/g of FPase, 83.15 U/g for ß-glucosidase,523.10 U/g of xylanase and 164.04 U/g of reducing sugar respectively. Cellulase and xylanase cocktail produced from these five fungi were mixed and applied on the Ganoderma boninense at ratio 1:1:1:1:1 were made up to 0.025mL in the culture plates. However, the inhibition of Ganoderma boninense when treated with cellulase and xylanase cocktail was undetected in this study. 2014 Thesis http://eprints.utm.my/id/eprint/48605/ http://eprints.utm.my/id/eprint/48605/1/RohayaMohdNoorMFBME2014.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:83226?queryType=vitalDismax&query=Screening+of+cellulolytic+and+xylanolytic+fungi+for+enzymes+cocktail+studies+of+ganoderma+boninense+inhibition&public=true masters Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering Faculty of Bioscience and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QK Botany
spellingShingle	QK Botany Mohd. Noor, Rohaya Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
description	Ganoderma boninense is a basidiomycetes white rot fungus which causes basal stem rot (BSR), one major disease in oil palm plantation in Malaysia. The objective of the present study was to screen the most potential fungus that has a capability to produce crude xylanase and cellulase to be used as a biological control for Ganoderma boninense. Twenty four different strains of fungi were obtained from the Biorefinery Technology Research Laboratory of Faculty Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor. They were screened for their potential to degrade cellulose and hemicelluloses. The screening of the fungi were based on the diameter of holozones on red color of congo red and the formation of yellow-opaque area on the carboxymethlycellulose(CMC) and xylan agar, respectively. Among these strains, five potential fungi which showed the maximum enzyme activities for endoglucanase (CMCase), filter paper degrading enzyme (FPase), beta-glucosidase, xylanase and reducing sugar were selected to produce enzyme cocktail on untreated oil palm trunk will be further study through its performance in solid state fermentation. The potential enzyme producers from fungi were CT2, Pycnoporus cinnabarinus, EFB1, EFB2 and TG6 that produced 304.04 U/g of CMCase, 13.25 U/g of FPase, 83.15 U/g for ß-glucosidase,523.10 U/g of xylanase and 164.04 U/g of reducing sugar respectively. Cellulase and xylanase cocktail produced from these five fungi were mixed and applied on the Ganoderma boninense at ratio 1:1:1:1:1 were made up to 0.025mL in the culture plates. However, the inhibition of Ganoderma boninense when treated with cellulase and xylanase cocktail was undetected in this study.
format	Thesis
qualification_level	Master's degree
author	Mohd. Noor, Rohaya
author_facet	Mohd. Noor, Rohaya
author_sort	Mohd. Noor, Rohaya
title	Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
title_short	Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
title_full	Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
title_fullStr	Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
title_full_unstemmed	Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
title_sort	screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Medical Engineering
granting_department	Faculty of Bioscience and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/48605/1/RohayaMohdNoorMFBME2014.pdf
_version_	1747817431231889408

Screening of cellulolytic and xylanolytic fungi for enzymes cocktail studies of ganoderma boninense inhibition

Similar Items