Biodegradation and metabolic pathway of cresol red dye by selected fungi

Massive amount of dye concentrations has been released into the environment as wastewater. Cresol red dyes remains as one of the most difficult dyes to be degraded. In this study, 20 different types of white rot fungi were isolated from a decayed wood in UTM forest and labelled as M 01-M 20. These f...

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Main Author:	Ahmad Zubir, Meor Mohd. Fikri
Format:	Thesis
Language:	English
Published:	2015
Subjects:	TA Engineering (General) Civil engineering (General)
Online Access:	http://eprints.utm.my/id/eprint/53582/1/MeorMohdFikriAhmadZubirMFKA2015.pdf
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spelling	my-utm-ep.535822020-07-22T01:04:54Z Biodegradation and metabolic pathway of cresol red dye by selected fungi 2015-06 Ahmad Zubir, Meor Mohd. Fikri TA Engineering (General). Civil engineering (General) Massive amount of dye concentrations has been released into the environment as wastewater. Cresol red dyes remains as one of the most difficult dyes to be degraded. In this study, 20 different types of white rot fungi were isolated from a decayed wood in UTM forest and labelled as M 01-M 20. These fungi were screened fortheir ability to degrade cresol red dye after 30 days of incubation. New metabolic pathways of degradation of cresol red dyes by fungi M06 and M15 were explored in this research. Both M06 and M15 were chosen based on their ability to decolorize 10 ppm cresol red dye on agar and liquid medium. These fungi were further tested for their ability to degrade cresol red dye inliquid medium under several conditions which is carbon and nitrogen sources, pH, salinity and effect of different concentration of surfactant (Tween 80). From decolorization, M06 showed efficient decolorization with addition of glucose (60.01%), ammonium sulphate (67.22%), Tween 80 0.1 mL (53.82%), 50 g/L of NaCl concentration (59.64%) and pH 3 (67.50%). Meanwhile, M15 showed efficient degradation of cresol red dye with addition of glucose (72.85%), ammonium nitrate (82.40%), Tween 80 0.1 mL (74.35%), 150 g/L of NaCl concentration (78.64%) and pH 3 (85.46%). These fungi were then identified using 18s rRNA sequence analysis method. From the phylogenetic tree, fungi M06 belongs to Trichdermaharzianum and fungi M15 belongs to Absidiaspinosa. Identification of metabolites was performed by combining several series of experiment through the use of UV-vis Spectrophotometer, Thin Layer Chromatography, and Gas Chromatography Mass Spectrophotometer. The analytical results showed that 2-hydrozybenzoic acid, and 2, 4-dyhidroxybenzoic acid were formed during the degradation of cresol red dye by TrichodermaharzianumM06. In the case of AbsidiaspinosaM15, benzoic acid and benzeneacetic acid were identified as the metabolic products produced during the degradation of cresol red dye. From the study, it is found that M 06 and M 15 have the capability to degrade cresol red dye.For further investigation, several experiments can be carried out to enhance the use of the isolated fungi in textile wastewater treatment. 2015-06 Thesis http://eprints.utm.my/id/eprint/53582/ http://eprints.utm.my/id/eprint/53582/1/MeorMohdFikriAhmadZubirMFKA2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:85650 masters Universiti Teknologi Malaysia, Faculty of Civil Engineering Faculty of Civil Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TA Engineering (General) Civil engineering (General)
spellingShingle	TA Engineering (General) Civil engineering (General) Ahmad Zubir, Meor Mohd. Fikri Biodegradation and metabolic pathway of cresol red dye by selected fungi
description	Massive amount of dye concentrations has been released into the environment as wastewater. Cresol red dyes remains as one of the most difficult dyes to be degraded. In this study, 20 different types of white rot fungi were isolated from a decayed wood in UTM forest and labelled as M 01-M 20. These fungi were screened fortheir ability to degrade cresol red dye after 30 days of incubation. New metabolic pathways of degradation of cresol red dyes by fungi M06 and M15 were explored in this research. Both M06 and M15 were chosen based on their ability to decolorize 10 ppm cresol red dye on agar and liquid medium. These fungi were further tested for their ability to degrade cresol red dye inliquid medium under several conditions which is carbon and nitrogen sources, pH, salinity and effect of different concentration of surfactant (Tween 80). From decolorization, M06 showed efficient decolorization with addition of glucose (60.01%), ammonium sulphate (67.22%), Tween 80 0.1 mL (53.82%), 50 g/L of NaCl concentration (59.64%) and pH 3 (67.50%). Meanwhile, M15 showed efficient degradation of cresol red dye with addition of glucose (72.85%), ammonium nitrate (82.40%), Tween 80 0.1 mL (74.35%), 150 g/L of NaCl concentration (78.64%) and pH 3 (85.46%). These fungi were then identified using 18s rRNA sequence analysis method. From the phylogenetic tree, fungi M06 belongs to Trichdermaharzianum and fungi M15 belongs to Absidiaspinosa. Identification of metabolites was performed by combining several series of experiment through the use of UV-vis Spectrophotometer, Thin Layer Chromatography, and Gas Chromatography Mass Spectrophotometer. The analytical results showed that 2-hydrozybenzoic acid, and 2, 4-dyhidroxybenzoic acid were formed during the degradation of cresol red dye by TrichodermaharzianumM06. In the case of AbsidiaspinosaM15, benzoic acid and benzeneacetic acid were identified as the metabolic products produced during the degradation of cresol red dye. From the study, it is found that M 06 and M 15 have the capability to degrade cresol red dye.For further investigation, several experiments can be carried out to enhance the use of the isolated fungi in textile wastewater treatment.
format	Thesis
qualification_level	Master's degree
author	Ahmad Zubir, Meor Mohd. Fikri
author_facet	Ahmad Zubir, Meor Mohd. Fikri
author_sort	Ahmad Zubir, Meor Mohd. Fikri
title	Biodegradation and metabolic pathway of cresol red dye by selected fungi
title_short	Biodegradation and metabolic pathway of cresol red dye by selected fungi
title_full	Biodegradation and metabolic pathway of cresol red dye by selected fungi
title_fullStr	Biodegradation and metabolic pathway of cresol red dye by selected fungi
title_full_unstemmed	Biodegradation and metabolic pathway of cresol red dye by selected fungi
title_sort	biodegradation and metabolic pathway of cresol red dye by selected fungi
granting_institution	Universiti Teknologi Malaysia, Faculty of Civil Engineering
granting_department	Faculty of Civil Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/53582/1/MeorMohdFikriAhmadZubirMFKA2015.pdf
_version_	1747817587168772096

Biodegradation and metabolic pathway of cresol red dye by selected fungi

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