Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis

Nowadays, the personal care and cosmetics market is one of the largest markets in the world. However, some potential cosmetic ingredients may cause skin sensitization. Animal testing is deemed as a perfect but controversial solution to skin sensitization analysis. The European Union (EU), which has...

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Main Author:	Noh, Teh Ubaidah
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/53717/25/TehUbaidahNohMFChE2014.pdf
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spelling	my-utm-ep.537172020-09-02T02:43:57Z Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis 2014-10 Noh, Teh Ubaidah TP Chemical technology Nowadays, the personal care and cosmetics market is one of the largest markets in the world. However, some potential cosmetic ingredients may cause skin sensitization. Animal testing is deemed as a perfect but controversial solution to skin sensitization analysis. The European Union (EU), which has the most stringent and protective regulations for cosmetic has agreed to ban all tests related to animals. This has wide ranging implications for cosmetic companies worldwide as a cosmetic product which has been successfully registered in the EU can be easily registered worldwide. Thus, in chemico, in silico or in vitro alternative methods for the prediction of skin sensitization need to be introduced. The main purpose of this work was to investigate the performance of an impedance skin sensitizer biosensor obtained using self-assembly of cysteine on screen printed carbon electrode (SPCE) modified with gold nanoparticles (AuNPs). The basis of the biosensor developed in this work was that the conjugation of allergen to cysteine-AuNPs on SPCE would result in the commencement of the skin sensitization process. A biosensor with good reproducibility (relative standard deviations of 8.43 %) and sensitivity was obtained when 50 mM of cysteine was deposited on the AuNPs and left for 24 hours on the SPCE. The biosensor managed to successfully differentiate between water soluble mild, medium and strong sensitizers based on the values of the changes in charge transfer resistance ( ΔRct). Different allergen concentrations did not significantly affect ΔRct readings (the range studied was between 10 to 90 mM). The biosensor in this research work was found to have the potential to be successfully used as an alternative method to animal testing for the detection of skin sensitizers. 2014-10 Thesis http://eprints.utm.my/id/eprint/53717/ http://eprints.utm.my/id/eprint/53717/25/TehUbaidahNohMFChE2014.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Noh, Teh Ubaidah Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
description	Nowadays, the personal care and cosmetics market is one of the largest markets in the world. However, some potential cosmetic ingredients may cause skin sensitization. Animal testing is deemed as a perfect but controversial solution to skin sensitization analysis. The European Union (EU), which has the most stringent and protective regulations for cosmetic has agreed to ban all tests related to animals. This has wide ranging implications for cosmetic companies worldwide as a cosmetic product which has been successfully registered in the EU can be easily registered worldwide. Thus, in chemico, in silico or in vitro alternative methods for the prediction of skin sensitization need to be introduced. The main purpose of this work was to investigate the performance of an impedance skin sensitizer biosensor obtained using self-assembly of cysteine on screen printed carbon electrode (SPCE) modified with gold nanoparticles (AuNPs). The basis of the biosensor developed in this work was that the conjugation of allergen to cysteine-AuNPs on SPCE would result in the commencement of the skin sensitization process. A biosensor with good reproducibility (relative standard deviations of 8.43 %) and sensitivity was obtained when 50 mM of cysteine was deposited on the AuNPs and left for 24 hours on the SPCE. The biosensor managed to successfully differentiate between water soluble mild, medium and strong sensitizers based on the values of the changes in charge transfer resistance ( ΔRct). Different allergen concentrations did not significantly affect ΔRct readings (the range studied was between 10 to 90 mM). The biosensor in this research work was found to have the potential to be successfully used as an alternative method to animal testing for the detection of skin sensitizers.
format	Thesis
qualification_level	Master's degree
author	Noh, Teh Ubaidah
author_facet	Noh, Teh Ubaidah
author_sort	Noh, Teh Ubaidah
title	Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
title_short	Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
title_full	Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
title_fullStr	Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
title_full_unstemmed	Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
title_sort	disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/53717/25/TehUbaidahNohMFChE2014.pdf
_version_	1747817620184236032

Disposable cysteine based electrochemical impedance biosensor for skin sensitization analysis

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