Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent

Heavy metal pollution has always been considered as one of the major threats to the environment and human health since these metals can accumulate in the food chain, inactivate cellular enzymes and may cause cancer related diseases. Conventional physiochemical methods do not provide economical treat...

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Main Author:	Wong, Siew Chiao
Format:	Thesis
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/53838/1/WongSiewChiaoMFBME2015.pdf
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spelling	my-utm-ep.538382020-09-07T02:36:14Z Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent 2015-08 Wong, Siew Chiao R Medicine (General) Heavy metal pollution has always been considered as one of the major threats to the environment and human health since these metals can accumulate in the food chain, inactivate cellular enzymes and may cause cancer related diseases. Conventional physiochemical methods do not provide economical treatment for the removal of heavy metals from heavy metal polluted environment. An effective and economical alternative method that has been widely reported is microbial bioremediation. In this study, the minimal inhibitory concentration (MIC) of Lysinibacillus fusiformis ZB2 for selected heavy metals, namely, cadmium (Cd), zinc (Zn), lead (Pb) and chromium (Cr(VI)) were determined. This bacteria was isolated previously from the textile effluent. It was grown in low phosphate medium (LPM) with glucose and tryptone as its carbon and nitrogen source respectively. L. fusiformis ZB2 reached its exponential growth within 48 hours of incubation in the LPM. The MIC of the bacteria for Cd, Zn, Pb and Cr(VI) were determined in solid and liquid media. The MIC obtained was relatively higher when using the liquid media. The MIC for Cd, Zn, Pb and Cr(VI) were 25, 75, 150, and 3500 ppm, respectively as compared to using the solid media MIC for Cd, Zn, Pb and Cr(VI) were 10, 75, 250, 3000 ppm, respectively. The order of toxicity of heavy metals towards Lysinibacillus fusiformis ZB2 was Cd>Zn>Pb>Cr(VI). The bacteria was found to be tolerant towards Zn, Pb and Cr(VI) with maximum tolerance towards Cr(VI). 2015-08 Thesis http://eprints.utm.my/id/eprint/53838/ http://eprints.utm.my/id/eprint/53838/1/WongSiewChiaoMFBME2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86676 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Wong, Siew Chiao Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent
description	Heavy metal pollution has always been considered as one of the major threats to the environment and human health since these metals can accumulate in the food chain, inactivate cellular enzymes and may cause cancer related diseases. Conventional physiochemical methods do not provide economical treatment for the removal of heavy metals from heavy metal polluted environment. An effective and economical alternative method that has been widely reported is microbial bioremediation. In this study, the minimal inhibitory concentration (MIC) of Lysinibacillus fusiformis ZB2 for selected heavy metals, namely, cadmium (Cd), zinc (Zn), lead (Pb) and chromium (Cr(VI)) were determined. This bacteria was isolated previously from the textile effluent. It was grown in low phosphate medium (LPM) with glucose and tryptone as its carbon and nitrogen source respectively. L. fusiformis ZB2 reached its exponential growth within 48 hours of incubation in the LPM. The MIC of the bacteria for Cd, Zn, Pb and Cr(VI) were determined in solid and liquid media. The MIC obtained was relatively higher when using the liquid media. The MIC for Cd, Zn, Pb and Cr(VI) were 25, 75, 150, and 3500 ppm, respectively as compared to using the solid media MIC for Cd, Zn, Pb and Cr(VI) were 10, 75, 250, 3000 ppm, respectively. The order of toxicity of heavy metals towards Lysinibacillus fusiformis ZB2 was Cd>Zn>Pb>Cr(VI). The bacteria was found to be tolerant towards Zn, Pb and Cr(VI) with maximum tolerance towards Cr(VI).
format	Thesis
qualification_level	Master's degree
author	Wong, Siew Chiao
author_facet	Wong, Siew Chiao
author_sort	Wong, Siew Chiao
title	Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent
title_short	Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent
title_full	Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent
title_fullStr	Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent
title_full_unstemmed	Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent
title_sort	heavy metal tolerance of lysinibacillus fusiformis zb2 isolated from textile effluent
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/53838/1/WongSiewChiaoMFBME2015.pdf
_version_	1747817639024001024

Heavy metal tolerance of lysinibacillus fusiformis ZB2 isolated from textile effluent

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