Production and optimization of indole-3-actic acid by rhodopseudomonas palustris

Indole-3-acetic acid (IAA) is known to be an important phytohormone that helps to regulate plant growth and development. In this study, the optimum culture medium for the production of IAA by Rhodopseusomonaspalustris in shake flask culture was studied. Rhodopseudomonaspalustris is a purple non-sulf...

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Main Author:	Leong, Pei Pei
Format:	Thesis
Language:	English
Published:	2015
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/53879/1/LeongPeiPeiMFKChE2015.pdf
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spelling	my-utm-ep.538792020-10-07T08:24:15Z Production and optimization of indole-3-actic acid by rhodopseudomonas palustris 2015-07 Leong, Pei Pei TP Chemical technology Indole-3-acetic acid (IAA) is known to be an important phytohormone that helps to regulate plant growth and development. In this study, the optimum culture medium for the production of IAA by Rhodopseusomonaspalustris in shake flask culture was studied. Rhodopseudomonaspalustris is a purple non-sulfurbacteria which has been well recognised as one of the most metabolitically versatile bacteria. The research was divided into three parts. First, a pre-screening process based on Taguchi Design was conducted to identify the significant factors that could affect the production of IAA. The pre-screening indicated that three parameters were found to be significant, which include the concentration of tryptophan, glucose and potassium nitrate. These parameters were selected and used to optimize the production IAA by Response Surface Methodology (RSM). Lastly, a kinetic study for the bacterial growth and IAA production was investigated. The optimal amount of IAA was obtained after incubation of 48 hours at 35 °C in the presence of 5 g L-1 of tryptophan, 4.94 g L-1 of glucose and 0.60 g L-1 of KNO3, as recommended by the RSM. Under this condition, the experimental yield of IAA production was 80.77? 2.13 µg mL-1, which was in close agreement with the value predicted by the RSM model (77.64 µg mL-1). This was the highest yield of IAA that was reported compared to the IAA yields obtained from the 20 experiments designed under the RSM. The IAA production depends on growth stage as most of the IAA was produced during the stationary growth phase of Rhodopseudomonaspalustris. This study has successfully optimized the production of IAA by Rhodopseudomonaspalustris by statistical approach and proved that Rhodopaeudomonaspalustris has the potential to be used as plant bioenhancer or biofertiliser for plant growth development. 2015-07 Thesis http://eprints.utm.my/id/eprint/53879/ http://eprints.utm.my/id/eprint/53879/1/LeongPeiPeiMFKChE2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86541 masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Leong, Pei Pei Production and optimization of indole-3-actic acid by rhodopseudomonas palustris
description	Indole-3-acetic acid (IAA) is known to be an important phytohormone that helps to regulate plant growth and development. In this study, the optimum culture medium for the production of IAA by Rhodopseusomonaspalustris in shake flask culture was studied. Rhodopseudomonaspalustris is a purple non-sulfurbacteria which has been well recognised as one of the most metabolitically versatile bacteria. The research was divided into three parts. First, a pre-screening process based on Taguchi Design was conducted to identify the significant factors that could affect the production of IAA. The pre-screening indicated that three parameters were found to be significant, which include the concentration of tryptophan, glucose and potassium nitrate. These parameters were selected and used to optimize the production IAA by Response Surface Methodology (RSM). Lastly, a kinetic study for the bacterial growth and IAA production was investigated. The optimal amount of IAA was obtained after incubation of 48 hours at 35 °C in the presence of 5 g L-1 of tryptophan, 4.94 g L-1 of glucose and 0.60 g L-1 of KNO3, as recommended by the RSM. Under this condition, the experimental yield of IAA production was 80.77? 2.13 µg mL-1, which was in close agreement with the value predicted by the RSM model (77.64 µg mL-1). This was the highest yield of IAA that was reported compared to the IAA yields obtained from the 20 experiments designed under the RSM. The IAA production depends on growth stage as most of the IAA was produced during the stationary growth phase of Rhodopseudomonaspalustris. This study has successfully optimized the production of IAA by Rhodopseudomonaspalustris by statistical approach and proved that Rhodopaeudomonaspalustris has the potential to be used as plant bioenhancer or biofertiliser for plant growth development.
format	Thesis
qualification_level	Master's degree
author	Leong, Pei Pei
author_facet	Leong, Pei Pei
author_sort	Leong, Pei Pei
title	Production and optimization of indole-3-actic acid by rhodopseudomonas palustris
title_short	Production and optimization of indole-3-actic acid by rhodopseudomonas palustris
title_full	Production and optimization of indole-3-actic acid by rhodopseudomonas palustris
title_fullStr	Production and optimization of indole-3-actic acid by rhodopseudomonas palustris
title_full_unstemmed	Production and optimization of indole-3-actic acid by rhodopseudomonas palustris
title_sort	production and optimization of indole-3-actic acid by rhodopseudomonas palustris
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/53879/1/LeongPeiPeiMFKChE2015.pdf
_version_	1747817648591208448

Production and optimization of indole-3-actic acid by rhodopseudomonas palustris

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