Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions

In recent studies, cold-active enzyme has become another subject of interest. It can overcome the limitations of the classical industrial enzymes that normally require higher temperature to function. In this study, 16 bacteria isolated from the Arctic region were screened for their protease activity...

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Main Author:	Liew, Kok Jun
Format:	Thesis
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/53969/1/LiewKokJunMFBME2015.pdf
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id	my-utm-ep.53969
record_format	uketd_dc
spelling	my-utm-ep.539692020-10-11T07:38:26Z Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions 2015-07 Liew, Kok Jun R Medicine (General) In recent studies, cold-active enzyme has become another subject of interest. It can overcome the limitations of the classical industrial enzymes that normally require higher temperature to function. In this study, 16 bacteria isolated from the Arctic region were screened for their protease activity using Skim Milk agar plate. Two bacteria with lower hydrolysis coefficient (Hc) were selected, they were Pseudomonas sp. 16D4 (Hc = 0.339) and Dermacoccus sp. 17D6 (Hc = 0.331), and their proteases were produced using “Protease Specific Medium” and were further studied. Both bacteria produced proteases that are associated to growth and the maximal protease activity was reached at 30th hour and 24th hours of incubation, respectively. Protease produced by Pseudomonas sp. 16D4 remained stable at temperature ranging from 0oC to 70oC. Its optimum activity was detected at 0oC, pH 9 or 11 with Km value of 1.37 mg/mL and Vmax value of 243.90 Unit/L. As for Dermacoccus sp. 17D6, the protease activity remained stable at temperature ranging from 0oC to 40oC and its optimum condition for the assay was at 50oC, pH 7 or 9 with Km value of 0.66 mg/mL and Vmax value of 344.83 Unit/L. The SDS-PAGE and zymogram analysis further revealed that the molecular mass of the Pseudomonas sp. 16D4’s protease was 40 kDa. Whereas the size for the Dermacoccus sp. 17D6’s protease cannot be determined due to the negative result obtained in the zymogram analysis. 2015-07 Thesis http://eprints.utm.my/id/eprint/53969/ http://eprints.utm.my/id/eprint/53969/1/LiewKokJunMFBME2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:85463 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Liew, Kok Jun Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions
description	In recent studies, cold-active enzyme has become another subject of interest. It can overcome the limitations of the classical industrial enzymes that normally require higher temperature to function. In this study, 16 bacteria isolated from the Arctic region were screened for their protease activity using Skim Milk agar plate. Two bacteria with lower hydrolysis coefficient (Hc) were selected, they were Pseudomonas sp. 16D4 (Hc = 0.339) and Dermacoccus sp. 17D6 (Hc = 0.331), and their proteases were produced using “Protease Specific Medium” and were further studied. Both bacteria produced proteases that are associated to growth and the maximal protease activity was reached at 30th hour and 24th hours of incubation, respectively. Protease produced by Pseudomonas sp. 16D4 remained stable at temperature ranging from 0oC to 70oC. Its optimum activity was detected at 0oC, pH 9 or 11 with Km value of 1.37 mg/mL and Vmax value of 243.90 Unit/L. As for Dermacoccus sp. 17D6, the protease activity remained stable at temperature ranging from 0oC to 40oC and its optimum condition for the assay was at 50oC, pH 7 or 9 with Km value of 0.66 mg/mL and Vmax value of 344.83 Unit/L. The SDS-PAGE and zymogram analysis further revealed that the molecular mass of the Pseudomonas sp. 16D4’s protease was 40 kDa. Whereas the size for the Dermacoccus sp. 17D6’s protease cannot be determined due to the negative result obtained in the zymogram analysis.
format	Thesis
qualification_level	Master's degree
author	Liew, Kok Jun
author_facet	Liew, Kok Jun
author_sort	Liew, Kok Jun
title	Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions
title_short	Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions
title_full	Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions
title_fullStr	Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions
title_full_unstemmed	Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions
title_sort	characterization of extracellular proteases from pseudomonas sp. 16d4 and dermacoccus sp. 17d6 isolated from arctic regions
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/53969/1/LiewKokJunMFBME2015.pdf
_version_	1747817664018907136

Characterization of extracellular proteases from Pseudomonas sp. 16D4 and Dermacoccus sp. 17D6 isolated from arctic regions

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