Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas

The aim of the study is to evaluate the effect of amino acid (tryptophan and glutamine) on the callus induction and to develop suspension cell culture protocol for Malaysian upland rice, Panderas cultivar. The research revealed that callus induction varied depend on amino acid tested. In callus indu...

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Main Author:	P. Rajadran, Nisha
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/53973/1/NishaPRajadranMFBME2015.pdf
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id	my-utm-ep.53973
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spelling	my-utm-ep.539732020-10-11T07:58:42Z Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas 2015-08 P. Rajadran, Nisha Q Science (General) The aim of the study is to evaluate the effect of amino acid (tryptophan and glutamine) on the callus induction and to develop suspension cell culture protocol for Malaysian upland rice, Panderas cultivar. The research revealed that callus induction varied depend on amino acid tested. In callus induction study, dehusked Panderas mature seeds were placed on MSB5 medium supplemented with 3mg/L 2,4-D and 2mg/L NAA with four different concentrations of tryptophan and glutamine separately. After three weeks in culture, inclusion of tryptophan showed positive effect on percentage of callus induction and fresh weight. It was observed that the optimum concentration of tryptophan was 25mg/L with 96.67% of callus induction and 73.33mg of average fresh weight. At week six, the average fresh weight of callus induced on MSB5 media supplemented with 25mg/L tryptophan was increased to three fold (215mg). Treatment without amino acids (control) showed similar callus percentage (96.67%) and three times increased in fresh weight after six weeks. However addition of 50mg/L glutamine alone showed lower percentage of callus induction and fresh weight compare to tryptophan and control treatment. The callus proliferation resulted in yellowish colour and nodular appearance showing embryogenic potential. The embryogenic callus was then immersed in 1% Evans blue to validate the viability of cells. Five weeks old potential embryogenic callus was then selected to initiate suspension cell culture. The N6 liquid medium supplemented with 3mg/L 2,4-D, 1mg/L kinetin and 0.005% pectinase resulted in higher fresh weight (1.84g) of suspension cells compared to without pectinase on day ten of incubation. This study concludes that tryptophan and glutamine did not show significant response on percentage of callus induction and fresh weight. Besides that inclusion of pectinase in suspension cell culture may increase fresh weight of suspension cell. 2015-08 Thesis http://eprints.utm.my/id/eprint/53973/ http://eprints.utm.my/id/eprint/53973/1/NishaPRajadranMFBME2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:85460 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) P. Rajadran, Nisha Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas
description	The aim of the study is to evaluate the effect of amino acid (tryptophan and glutamine) on the callus induction and to develop suspension cell culture protocol for Malaysian upland rice, Panderas cultivar. The research revealed that callus induction varied depend on amino acid tested. In callus induction study, dehusked Panderas mature seeds were placed on MSB5 medium supplemented with 3mg/L 2,4-D and 2mg/L NAA with four different concentrations of tryptophan and glutamine separately. After three weeks in culture, inclusion of tryptophan showed positive effect on percentage of callus induction and fresh weight. It was observed that the optimum concentration of tryptophan was 25mg/L with 96.67% of callus induction and 73.33mg of average fresh weight. At week six, the average fresh weight of callus induced on MSB5 media supplemented with 25mg/L tryptophan was increased to three fold (215mg). Treatment without amino acids (control) showed similar callus percentage (96.67%) and three times increased in fresh weight after six weeks. However addition of 50mg/L glutamine alone showed lower percentage of callus induction and fresh weight compare to tryptophan and control treatment. The callus proliferation resulted in yellowish colour and nodular appearance showing embryogenic potential. The embryogenic callus was then immersed in 1% Evans blue to validate the viability of cells. Five weeks old potential embryogenic callus was then selected to initiate suspension cell culture. The N6 liquid medium supplemented with 3mg/L 2,4-D, 1mg/L kinetin and 0.005% pectinase resulted in higher fresh weight (1.84g) of suspension cells compared to without pectinase on day ten of incubation. This study concludes that tryptophan and glutamine did not show significant response on percentage of callus induction and fresh weight. Besides that inclusion of pectinase in suspension cell culture may increase fresh weight of suspension cell.
format	Thesis
qualification_level	Master's degree
author	P. Rajadran, Nisha
author_facet	P. Rajadran, Nisha
author_sort	P. Rajadran, Nisha
title	Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas
title_short	Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas
title_full	Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas
title_fullStr	Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas
title_full_unstemmed	Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas
title_sort	callus induction and development of suspension cell culture of malaysian upland rice cultivar panderas
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/53973/1/NishaPRajadranMFBME2015.pdf
_version_	1747817664999325696

Callus induction and development of suspension cell culture of Malaysian upland rice cultivar Panderas

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