Protein profiling of Orthosiphon stamineus

Orthosiphon stamineus (cat whiskers) is a medicinal herb belonging to the family of Lamiaceae, where the leaves are commonly used as herbal tea known as “Java tea”. It is used as a diuretic agent and for the treatment of heat rheumatism. Currently there is no protein profile data available for this...

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Main Author:	Usman, Kamal
Format:	Thesis
Language:	English
Published:	2014
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/54015/25/KamalUsmanMFBME2014.pdf
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id	my-utm-ep.54015
record_format	uketd_dc
spelling	my-utm-ep.540152020-10-14T09:12:55Z Protein profiling of Orthosiphon stamineus 2014-12 Usman, Kamal Q Science (General) Orthosiphon stamineus (cat whiskers) is a medicinal herb belonging to the family of Lamiaceae, where the leaves are commonly used as herbal tea known as “Java tea”. It is used as a diuretic agent and for the treatment of heat rheumatism. Currently there is no protein profile data available for this important plant species. This study focus on the optimization of total protein extraction by employing four different methods of extraction protocols; QB buffer, Phenol/SDS with three prewashed steps, Phenol/SDS without prewashed steps and Sigma protein extraction kit with the aim of determining the best protein extraction method, protein pattern of the two varieties of O.stamineus (white and purple flower) and bioinformatics analysis to identify the proteins. Overall, Phenol/SDS with three prewashed steps result in a better protein quality and pattern of separation for both plant varieties.A total of 104 functional proteins were identified with each containing at least one unique peptide. Among the identified proteins, Rubisco activase and Triosephosphate isomerase corresponds to the chloroplastic protein of photosynthesis/carbohydrate metabolism while Phosphoglycerate kinase and Glyceraldehyde are cytosolic enzymes of glycolysis pathway which were found to be major housekeeping proteins in a leaf tissue. The result of this study will provide an insight for advanced pharmaceutical research and a baseline information for further proteomics work on O. stamineus and similar plant species. 2014-12 Thesis http://eprints.utm.my/id/eprint/54015/ http://eprints.utm.my/id/eprint/54015/25/KamalUsmanMFBME2014.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86085 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Usman, Kamal Protein profiling of Orthosiphon stamineus
description	Orthosiphon stamineus (cat whiskers) is a medicinal herb belonging to the family of Lamiaceae, where the leaves are commonly used as herbal tea known as “Java tea”. It is used as a diuretic agent and for the treatment of heat rheumatism. Currently there is no protein profile data available for this important plant species. This study focus on the optimization of total protein extraction by employing four different methods of extraction protocols; QB buffer, Phenol/SDS with three prewashed steps, Phenol/SDS without prewashed steps and Sigma protein extraction kit with the aim of determining the best protein extraction method, protein pattern of the two varieties of O.stamineus (white and purple flower) and bioinformatics analysis to identify the proteins. Overall, Phenol/SDS with three prewashed steps result in a better protein quality and pattern of separation for both plant varieties.A total of 104 functional proteins were identified with each containing at least one unique peptide. Among the identified proteins, Rubisco activase and Triosephosphate isomerase corresponds to the chloroplastic protein of photosynthesis/carbohydrate metabolism while Phosphoglycerate kinase and Glyceraldehyde are cytosolic enzymes of glycolysis pathway which were found to be major housekeeping proteins in a leaf tissue. The result of this study will provide an insight for advanced pharmaceutical research and a baseline information for further proteomics work on O. stamineus and similar plant species.
format	Thesis
qualification_level	Master's degree
author	Usman, Kamal
author_facet	Usman, Kamal
author_sort	Usman, Kamal
title	Protein profiling of Orthosiphon stamineus
title_short	Protein profiling of Orthosiphon stamineus
title_full	Protein profiling of Orthosiphon stamineus
title_fullStr	Protein profiling of Orthosiphon stamineus
title_full_unstemmed	Protein profiling of Orthosiphon stamineus
title_sort	protein profiling of orthosiphon stamineus
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/54015/25/KamalUsmanMFBME2014.pdf
_version_	1747817674288660480

Protein profiling of Orthosiphon stamineus

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