Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples

The study on microorganisms living in extreme cold environment such as the arctic and antarctic regions is important for the biotechnological exploration and biodiversity. Such organisms however, could have enzymes capable of degrading complex molecules at low temperature. In this work, culturable b...

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Main Author:	Abba, Mustapha
Format:	Thesis
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/54597/25/MustaphaAbbaMFBME2015.pdf
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id	my-utm-ep.54597
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spelling	my-utm-ep.545972020-10-20T02:41:22Z Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples 2015-01 Abba, Mustapha R Medicine (General) The study on microorganisms living in extreme cold environment such as the arctic and antarctic regions is important for the biotechnological exploration and biodiversity. Such organisms however, could have enzymes capable of degrading complex molecules at low temperature. In this work, culturable bacteria from arctic soil and sediment samples have been isolated under low temperature (20ºC), using Antarctic Bacterium medium (ABM). A total of 40 different bacteria were isolated and screen for their ability to produce cellulase using carboxymethyl cellulose (CMC) as substrates. Cellulase producing bacteria were selected based on their hydrolytic coefficient. Molecular characterizations of the potent cellulase producing isolates were carried out using 16S rRNA gene sequence method. The effects of temperature, pH and salinity were tested on four bacterial isolates that showed high cellulase activity. However only two of the isolates (SL9 and SL 19) were able to retains their cellulolytic activity at 5%, 10%, and 15% salt concentrations. Based on the 16S rRNA gene sequence and the phylogenetic analysis, the four potential cellulolytic bacterial isolates were identified as Massilia sp. and Pseudomonas sp. The findings of this work suggested that arctic bacterial isolates such as Massilia and Peudomonas species could serve as promising agents for the production of bacterial cellulase with various biotechnological applications; such as food supplement, animal feed supplement as well as bioremediation of pharmaceutical wastewater. 2015-01 Thesis http://eprints.utm.my/id/eprint/54597/ http://eprints.utm.my/id/eprint/54597/25/MustaphaAbbaMFBME2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86686 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abba, Mustapha Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
description	The study on microorganisms living in extreme cold environment such as the arctic and antarctic regions is important for the biotechnological exploration and biodiversity. Such organisms however, could have enzymes capable of degrading complex molecules at low temperature. In this work, culturable bacteria from arctic soil and sediment samples have been isolated under low temperature (20ºC), using Antarctic Bacterium medium (ABM). A total of 40 different bacteria were isolated and screen for their ability to produce cellulase using carboxymethyl cellulose (CMC) as substrates. Cellulase producing bacteria were selected based on their hydrolytic coefficient. Molecular characterizations of the potent cellulase producing isolates were carried out using 16S rRNA gene sequence method. The effects of temperature, pH and salinity were tested on four bacterial isolates that showed high cellulase activity. However only two of the isolates (SL9 and SL 19) were able to retains their cellulolytic activity at 5%, 10%, and 15% salt concentrations. Based on the 16S rRNA gene sequence and the phylogenetic analysis, the four potential cellulolytic bacterial isolates were identified as Massilia sp. and Pseudomonas sp. The findings of this work suggested that arctic bacterial isolates such as Massilia and Peudomonas species could serve as promising agents for the production of bacterial cellulase with various biotechnological applications; such as food supplement, animal feed supplement as well as bioremediation of pharmaceutical wastewater.
format	Thesis
qualification_level	Master's degree
author	Abba, Mustapha
author_facet	Abba, Mustapha
author_sort	Abba, Mustapha
title	Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
title_short	Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
title_full	Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
title_fullStr	Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
title_full_unstemmed	Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
title_sort	screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/54597/25/MustaphaAbbaMFBME2015.pdf
_version_	1747817685069070336

Screening and characterization of cellulolytic bacteria isolated from arctic soil and sediment samples

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