Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens

Persicaria minor (Huds.) Opiz known as Small water-pepper and well recognized locally in Malaysia as “daun kesum” is an edible vegetable with nutritional and medicinal benefits utilized generally by South-east Asians. The present study was conducted to evaluate the antibacterial activity of standard...

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Main Author:	Abubakar, Musa Ahmed
Format:	Thesis
Language:	English
Published:	2015
Subjects:	R Medicine (General)
Online Access:	http://eprints.utm.my/id/eprint/54642/25/MusaAhmedAbubakarMFBME2015.pdf
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id	my-utm-ep.54642
record_format	uketd_dc
spelling	my-utm-ep.546422020-10-21T02:52:08Z Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens 2015-01 Abubakar, Musa Ahmed R Medicine (General) Persicaria minor (Huds.) Opiz known as Small water-pepper and well recognized locally in Malaysia as “daun kesum” is an edible vegetable with nutritional and medicinal benefits utilized generally by South-east Asians. The present study was conducted to evaluate the antibacterial activity of standardized aqueous-ethanolic and water extracts of P. minor leaves. The leaves of the plant undergone extraction based on Malaysian Standard Guideline which is 30% aqueousethanol and absolute water as normally used in traditional medicine to produce the respective extract concentrates. Both extracts were evaluated for total protein and polysaccharide contents in which aqueous-ethanolic extract was found to possess high contents of proteins (1713.67 µg/ml) while contents of polysaccharides were high in absolute water extract (17.6 µg/ml). These measurements were used as a standard for different batch extract. The extracts were then tested against four standard strains of bacteria which are Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 11229, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442 at different concentrations using discdiffusion (qualitative) and microplate dilution (quantitative) assays. For positive and negative control, penicillin and dimethylsulfoxide were used as controls, respectively. Both extracts showed antibacterial activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values in the range of 50 to 100 mg/mL against S. aureus, E. faecalis, and E. coli, respectively with aqueous-ethanolic extract being more potent. However, none of the extracts were active against P. aeruginosa. Results from this study truly illustrated high potential of P. minor leaves as natural antibacterial agent for the elimination of various bacterial disease and infections. 2015-01 Thesis http://eprints.utm.my/id/eprint/54642/ http://eprints.utm.my/id/eprint/54642/25/MusaAhmedAbubakarMFBME2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:86598 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abubakar, Musa Ahmed Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens
description	Persicaria minor (Huds.) Opiz known as Small water-pepper and well recognized locally in Malaysia as “daun kesum” is an edible vegetable with nutritional and medicinal benefits utilized generally by South-east Asians. The present study was conducted to evaluate the antibacterial activity of standardized aqueous-ethanolic and water extracts of P. minor leaves. The leaves of the plant undergone extraction based on Malaysian Standard Guideline which is 30% aqueousethanol and absolute water as normally used in traditional medicine to produce the respective extract concentrates. Both extracts were evaluated for total protein and polysaccharide contents in which aqueous-ethanolic extract was found to possess high contents of proteins (1713.67 µg/ml) while contents of polysaccharides were high in absolute water extract (17.6 µg/ml). These measurements were used as a standard for different batch extract. The extracts were then tested against four standard strains of bacteria which are Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 11229, Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442 at different concentrations using discdiffusion (qualitative) and microplate dilution (quantitative) assays. For positive and negative control, penicillin and dimethylsulfoxide were used as controls, respectively. Both extracts showed antibacterial activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values in the range of 50 to 100 mg/mL against S. aureus, E. faecalis, and E. coli, respectively with aqueous-ethanolic extract being more potent. However, none of the extracts were active against P. aeruginosa. Results from this study truly illustrated high potential of P. minor leaves as natural antibacterial agent for the elimination of various bacterial disease and infections.
format	Thesis
qualification_level	Master's degree
author	Abubakar, Musa Ahmed
author_facet	Abubakar, Musa Ahmed
author_sort	Abubakar, Musa Ahmed
title	Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens
title_short	Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens
title_full	Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens
title_fullStr	Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens
title_full_unstemmed	Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens
title_sort	antibacterial activity of persicaria minor (huds.) leaf-extracts against bacterial pathogens
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/54642/25/MusaAhmedAbubakarMFBME2015.pdf
_version_	1747817694600626176

Antibacterial activity of persicaria minor (Huds.) leaf-extracts against bacterial pathogens

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