Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion

Nowadays there is an intensely usage of natural bioactive materials as medicinal agent in pharmaceutical industries. Swietenia macrophylla (SM) has been reported to have biological activities such as anti-inflammatory, anti-mutagencity, anti-fungal and anti-tumor activities. Therefore, SM oil was us...

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Main Author:	Masoud Eid, Ahmad Mustafa
Format:	Thesis
Language:	English
Published:	2015
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/54705/1/AhmadMustafaMasoudEidPFChE2015.pdf
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spelling	my-utm-ep.547052020-11-03T08:52:35Z Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion 2015-05 Masoud Eid, Ahmad Mustafa TP Chemical technology Nowadays there is an intensely usage of natural bioactive materials as medicinal agent in pharmaceutical industries. Swietenia macrophylla (SM) has been reported to have biological activities such as anti-inflammatory, anti-mutagencity, anti-fungal and anti-tumor activities. Therefore, SM oil was used in the formulation of oral nanoemulsion as self-nanoemulsifying system and topical nanoemulsion as nanoemulgel. In this study, self-nanoemulsifying formulations were prepared for both SM and olive oils using different surfactants/co-surfactants nonionic surfactants (Labrasol, Tween 20, Capmul and Labrafil). For topical preparation, nanoemulgels of SM and olive oils were prepared, first by mixing the oil, glycerol with sucrose ester (Laureate, Oleate and Palmitate) to produce pre-nanoemulsion by heat inversion technique. Then nanoemulsion was formed upon the mixing with water by selfemulsification technique. After that Carbopol (934 and 940) was added to nanoemulsion to produce the nanoemulgel. Finally safety and pharmacology studies were conducted for the prepared formulations. The acute and sub-acute toxicity studies were conducted for SM oil self-nanoemulsifying system on rats. The biochemical parameters were evaluated by studying serum alanine transferase (ALT), aspartate transferase (AST) and alkaline phosphatase (ALP). The histopathology of the rats liver sections were studied under light microscope after staining with haematoxylin and eosin. On the other hand, the anti-inflammatory property for SM oil was screened using carrageenan induced rat paw edema method for SM oil for both oral (self-nanoemulsifying system) and topical (nanoemulgel) preparations. The optimal self-nanoemulsifying formulations were prepared using Tween20/Labrafil (2:1) and Capmul/Labrasol (1:2), producing small droplets size of <150 nm, low polydispersity index below 0.3 and relatively good stability with zeta potential lower than -30. It was found that 50% oil with sucrose laureate (20%) and glycerol (30%) was able to produce a very stable nanoemulsion when stored at 4 °C with droplets size less than 150 nm, with low size distribution below 0.2 and low zeta potential -40. The optimal nanoemulsion formulation was mixed with water and different grades of Carbopol (934 and 940) to produce nanoemulgel. It was found that 0.5% Carbopol 940 produces a stable nanoemulgel and it showed high stability when stored at different temperatures 4 °C, 25 °C and 40 °C for one year with no significant influence on the oil droplets size, size distribution and zeta potential. In addition, the anti-inflammatory properties of Swietenia macrophylla oil were greater in the form of self-nanoemulsifying system and nanoemulgel than in the oil solution. Swietenia macrophylla oil in the form of self-nanoemulsifying system possesses no toxicity effect on rats after acute and sub-acute studies. In conclusion, Swietenia macrophylla oil showed a safety profile with improvement in the anti-inflammatory activity with the help of nanoemulsion. 2015-05 Thesis http://eprints.utm.my/id/eprint/54705/ http://eprints.utm.my/id/eprint/54705/1/AhmadMustafaMasoudEidPFChE2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:94648 phd doctoral Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Masoud Eid, Ahmad Mustafa Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
description	Nowadays there is an intensely usage of natural bioactive materials as medicinal agent in pharmaceutical industries. Swietenia macrophylla (SM) has been reported to have biological activities such as anti-inflammatory, anti-mutagencity, anti-fungal and anti-tumor activities. Therefore, SM oil was used in the formulation of oral nanoemulsion as self-nanoemulsifying system and topical nanoemulsion as nanoemulgel. In this study, self-nanoemulsifying formulations were prepared for both SM and olive oils using different surfactants/co-surfactants nonionic surfactants (Labrasol, Tween 20, Capmul and Labrafil). For topical preparation, nanoemulgels of SM and olive oils were prepared, first by mixing the oil, glycerol with sucrose ester (Laureate, Oleate and Palmitate) to produce pre-nanoemulsion by heat inversion technique. Then nanoemulsion was formed upon the mixing with water by selfemulsification technique. After that Carbopol (934 and 940) was added to nanoemulsion to produce the nanoemulgel. Finally safety and pharmacology studies were conducted for the prepared formulations. The acute and sub-acute toxicity studies were conducted for SM oil self-nanoemulsifying system on rats. The biochemical parameters were evaluated by studying serum alanine transferase (ALT), aspartate transferase (AST) and alkaline phosphatase (ALP). The histopathology of the rats liver sections were studied under light microscope after staining with haematoxylin and eosin. On the other hand, the anti-inflammatory property for SM oil was screened using carrageenan induced rat paw edema method for SM oil for both oral (self-nanoemulsifying system) and topical (nanoemulgel) preparations. The optimal self-nanoemulsifying formulations were prepared using Tween20/Labrafil (2:1) and Capmul/Labrasol (1:2), producing small droplets size of <150 nm, low polydispersity index below 0.3 and relatively good stability with zeta potential lower than -30. It was found that 50% oil with sucrose laureate (20%) and glycerol (30%) was able to produce a very stable nanoemulsion when stored at 4 °C with droplets size less than 150 nm, with low size distribution below 0.2 and low zeta potential -40. The optimal nanoemulsion formulation was mixed with water and different grades of Carbopol (934 and 940) to produce nanoemulgel. It was found that 0.5% Carbopol 940 produces a stable nanoemulgel and it showed high stability when stored at different temperatures 4 °C, 25 °C and 40 °C for one year with no significant influence on the oil droplets size, size distribution and zeta potential. In addition, the anti-inflammatory properties of Swietenia macrophylla oil were greater in the form of self-nanoemulsifying system and nanoemulgel than in the oil solution. Swietenia macrophylla oil in the form of self-nanoemulsifying system possesses no toxicity effect on rats after acute and sub-acute studies. In conclusion, Swietenia macrophylla oil showed a safety profile with improvement in the anti-inflammatory activity with the help of nanoemulsion.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Masoud Eid, Ahmad Mustafa
author_facet	Masoud Eid, Ahmad Mustafa
author_sort	Masoud Eid, Ahmad Mustafa
title	Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_short	Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_full	Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_fullStr	Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_full_unstemmed	Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
title_sort	improvement of anti-inflammation of swietenia macrophylla self nanoemulsion
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/54705/1/AhmadMustafaMasoudEidPFChE2015.pdf
_version_	1747817707291541504

Improvement of anti-inflammation of swietenia macrophylla self nanoemulsion

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