In vitro permeation and skin retention of alpha-mangostin proniosome

Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application...

Full description

Saved in:

Bibliographic Details
Main Author:	Gan, Siaw Chin
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.60702
record_format	uketd_dc
spelling	my-utm-ep.607022020-12-22T07:47:25Z In vitro permeation and skin retention of alpha-mangostin proniosome 2016-05 Gan, Siaw Chin TP Chemical technology Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application of a-mangostin in topical formulation is restricted. The current investigation aimed to evaluate the potential of proniosome as a carrier to enhance skin permeation and skin retention of a-mangostin. Alpha-mangostin proniosomal formulations were prepared using coacervation phase separation method. Upon hydration, a-mangostin-loaded niosomes were characterized for size, polydispersity index, entrapment efficiency, zeta potential and morphology. Using different surfactants, preliminary study to evaluate skin concentration suggested that Spans significantly (p < 0.05) enhanced deposition of a-mangostin in the viable epidermis/dermis layer (VED) as compared to Tween 60. Incorporation of soya lecithin in the proniosomal formulation also significantly enhanced the VED concentration of a-mangostin. The in vitro permeation experiments using dermis-split Yucatan Micropig skin revealed that proniosomes composed of Spans, soya lecithin and cholesterol were able to enhance the skin permeation of a-mangostin with a factor range from 1.8 to 8.0-fold as compared to the control suspension. All the proniosomal formulations (except for S20L) had significantly (p < 0.05) enhanced the deposition of a-mangostin in the VED layer with a factor range from 2.5 to 2.9-fold as compared to the control suspension. Proniosome S85L showed the highest permeation profile (8.0-fold) and the highest enhancement of VED concentration of a-mangostin (2.9-fold). Collectively, these results suggested that proniosomes can be utilized as a promising carrier for a highly lipophilic compound like a-mangostin. 2016-05 Thesis http://eprints.utm.my/id/eprint/60702/ http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:93953 masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Gan, Siaw Chin In vitro permeation and skin retention of alpha-mangostin proniosome
description	Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application of a-mangostin in topical formulation is restricted. The current investigation aimed to evaluate the potential of proniosome as a carrier to enhance skin permeation and skin retention of a-mangostin. Alpha-mangostin proniosomal formulations were prepared using coacervation phase separation method. Upon hydration, a-mangostin-loaded niosomes were characterized for size, polydispersity index, entrapment efficiency, zeta potential and morphology. Using different surfactants, preliminary study to evaluate skin concentration suggested that Spans significantly (p < 0.05) enhanced deposition of a-mangostin in the viable epidermis/dermis layer (VED) as compared to Tween 60. Incorporation of soya lecithin in the proniosomal formulation also significantly enhanced the VED concentration of a-mangostin. The in vitro permeation experiments using dermis-split Yucatan Micropig skin revealed that proniosomes composed of Spans, soya lecithin and cholesterol were able to enhance the skin permeation of a-mangostin with a factor range from 1.8 to 8.0-fold as compared to the control suspension. All the proniosomal formulations (except for S20L) had significantly (p < 0.05) enhanced the deposition of a-mangostin in the VED layer with a factor range from 2.5 to 2.9-fold as compared to the control suspension. Proniosome S85L showed the highest permeation profile (8.0-fold) and the highest enhancement of VED concentration of a-mangostin (2.9-fold). Collectively, these results suggested that proniosomes can be utilized as a promising carrier for a highly lipophilic compound like a-mangostin.
format	Thesis
qualification_level	Master's degree
author	Gan, Siaw Chin
author_facet	Gan, Siaw Chin
author_sort	Gan, Siaw Chin
title	In vitro permeation and skin retention of alpha-mangostin proniosome
title_short	In vitro permeation and skin retention of alpha-mangostin proniosome
title_full	In vitro permeation and skin retention of alpha-mangostin proniosome
title_fullStr	In vitro permeation and skin retention of alpha-mangostin proniosome
title_full_unstemmed	In vitro permeation and skin retention of alpha-mangostin proniosome
title_sort	in vitro permeation and skin retention of alpha-mangostin proniosome
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf
_version_	1747817762875506688

In vitro permeation and skin retention of alpha-mangostin proniosome

Similar Items