Alpha mangostin optimized extraction by supercritical carbon dioxide technique

Recently, there is an increasing interest to include plant derived compounds both in cosmetic and nutraceutical products. However, majority of these compounds are hard to isolate and some biologically active compounds are usually in low concentration. Furthermore, conventional extraction processes a...

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Main Author:	Abu Bakar, Norshamiza
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/77801/1/NorshamizaAbuBakarMFChE2016.pdf
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spelling	my-utm-ep.778012018-07-04T11:44:33Z Alpha mangostin optimized extraction by supercritical carbon dioxide technique 2016-06 Abu Bakar, Norshamiza TP Chemical technology Recently, there is an increasing interest to include plant derived compounds both in cosmetic and nutraceutical products. However, majority of these compounds are hard to isolate and some biologically active compounds are usually in low concentration. Furthermore, conventional extraction processes are mostly time consuming with high usage of toxic solvent. Thus, carbon dioxide (CO2) which generally recognized as safe solvent can be a promising alternative to extract the plant derived compounds. In this study, supercritical carbon dioxide (SC-CO2) extraction was compared with conventional soxhlet extraction for the extraction of α- mangostin from pericarps of Garcinia mangostana. The optimization was carried out using Box-Behnken Design. Three parameters were manipulated in SC-CO2 optimization process which included extraction pressure (20 to 30 MPa), extraction temperature (40 to 60 °C) and percentage of ethanol as co solvent (0 to 3%) at fixed sample particle size of 0.10-0.50 mm and 4 mL/min of CO2 flow rate. The analysis was carried out by using high performance liquid chromatography (HPLC). The optimum conditions for α-mangostin extraction (58% w/w) was at 20 MPa of pressure, temperature of 45 °C and 3% of ethanol as co solvent. Further isolation of SC-CO2 extracts using column chromatography succesfully isolated pure α- mangostin (1.42% w/w) based from spectral data of HPLC chromatogram, 1H and 13C nuclear magnetic resonance and mass spectrometry. It was found that SC-CO2 was more superior in terms of less toxic solvent used and more yield of α-mangostin extracted compared with the conventional and traditional soxhlet extraction technique. 2016-06 Thesis http://eprints.utm.my/id/eprint/77801/ http://eprints.utm.my/id/eprint/77801/1/NorshamizaAbuBakarMFChE2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:93952 masters Universiti Teknologi Malaysia, Faculty of Chemical and Energy Engineering Faculty of Chemical and Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abu Bakar, Norshamiza Alpha mangostin optimized extraction by supercritical carbon dioxide technique
description	Recently, there is an increasing interest to include plant derived compounds both in cosmetic and nutraceutical products. However, majority of these compounds are hard to isolate and some biologically active compounds are usually in low concentration. Furthermore, conventional extraction processes are mostly time consuming with high usage of toxic solvent. Thus, carbon dioxide (CO2) which generally recognized as safe solvent can be a promising alternative to extract the plant derived compounds. In this study, supercritical carbon dioxide (SC-CO2) extraction was compared with conventional soxhlet extraction for the extraction of α- mangostin from pericarps of Garcinia mangostana. The optimization was carried out using Box-Behnken Design. Three parameters were manipulated in SC-CO2 optimization process which included extraction pressure (20 to 30 MPa), extraction temperature (40 to 60 °C) and percentage of ethanol as co solvent (0 to 3%) at fixed sample particle size of 0.10-0.50 mm and 4 mL/min of CO2 flow rate. The analysis was carried out by using high performance liquid chromatography (HPLC). The optimum conditions for α-mangostin extraction (58% w/w) was at 20 MPa of pressure, temperature of 45 °C and 3% of ethanol as co solvent. Further isolation of SC-CO2 extracts using column chromatography succesfully isolated pure α- mangostin (1.42% w/w) based from spectral data of HPLC chromatogram, 1H and 13C nuclear magnetic resonance and mass spectrometry. It was found that SC-CO2 was more superior in terms of less toxic solvent used and more yield of α-mangostin extracted compared with the conventional and traditional soxhlet extraction technique.
format	Thesis
qualification_level	Master's degree
author	Abu Bakar, Norshamiza
author_facet	Abu Bakar, Norshamiza
author_sort	Abu Bakar, Norshamiza
title	Alpha mangostin optimized extraction by supercritical carbon dioxide technique
title_short	Alpha mangostin optimized extraction by supercritical carbon dioxide technique
title_full	Alpha mangostin optimized extraction by supercritical carbon dioxide technique
title_fullStr	Alpha mangostin optimized extraction by supercritical carbon dioxide technique
title_full_unstemmed	Alpha mangostin optimized extraction by supercritical carbon dioxide technique
title_sort	alpha mangostin optimized extraction by supercritical carbon dioxide technique
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical and Energy Engineering
granting_department	Faculty of Chemical and Energy Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/77801/1/NorshamizaAbuBakarMFChE2016.pdf
_version_	1747817834330718208

Alpha mangostin optimized extraction by supercritical carbon dioxide technique

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