Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius

Trigonelline was a plant hormone that is built up in a plant from the methylation of the nitrogen atom of niacin. It was responsible for induction of G2 phase arrest in the root apices of many plant species. Trigonelline was also capable of reducing the blood sugar level and thus treating diabetes t...

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Main Author:	Ramly, Ahmad Lutfi
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH Natural history
Online Access:	http://eprints.utm.my/id/eprint/77815/1/AhmadLutfiRamlyMFBME2016.pdf
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spelling	my-utm-ep.778152018-07-04T11:48:01Z Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius 2016-09 Ramly, Ahmad Lutfi QH Natural history Trigonelline was a plant hormone that is built up in a plant from the methylation of the nitrogen atom of niacin. It was responsible for induction of G2 phase arrest in the root apices of many plant species. Trigonelline was also capable of reducing the blood sugar level and thus treating diabetes type II. In this study, effect of methyl jasmonate, MeJa (50 µM, 100 µM and 200 µM) on trigonelline content in suspension cell culture of Abrus precatorius using High Performance –Liquid Chromatography for trigonelline was investigated. The effect of different inoculum size (0.1 g and 0.5 g) on cell biomass and trigonelline content of suspension cell culture of A.precatorius was studied. Suspension cell culture of A. precatorius was developed in a 100 mL conical flask containing 30 mL MS medium supplemented with 0.5 mg/L kinetin and 0.5 mg/L 2, 4- D. Results showed that 0.5 g inoculum had higher cell biomass with maximum biomass of 0.0795 g than 0.1 g inoculum that had biomass decrement throughout the culture. Furthermore, addition of 50 µM MeJa had multiplied trigonelline content to five times (6.62 mg/L) compared to the control (1.41 mg/L) for a period of 2 weeks. The correlation coefficient test indicated that cell biomass was not correlated with trigonelline content. Different increment of cell biomass with trigonelline suggested that trigonelline was not involved in regulating cell cycle of A. precatorius. In conclusion, 0.5 g inoculum size was a better size compared to 0.1 g inoculum suspension cell culture. The optimum trigonelline during cell growth of A.precatorius was also discovered. 2016-09 Thesis http://eprints.utm.my/id/eprint/77815/ http://eprints.utm.my/id/eprint/77815/1/AhmadLutfiRamlyMFBME2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:103448 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH Natural history
spellingShingle	QH Natural history Ramly, Ahmad Lutfi Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius
description	Trigonelline was a plant hormone that is built up in a plant from the methylation of the nitrogen atom of niacin. It was responsible for induction of G2 phase arrest in the root apices of many plant species. Trigonelline was also capable of reducing the blood sugar level and thus treating diabetes type II. In this study, effect of methyl jasmonate, MeJa (50 µM, 100 µM and 200 µM) on trigonelline content in suspension cell culture of Abrus precatorius using High Performance –Liquid Chromatography for trigonelline was investigated. The effect of different inoculum size (0.1 g and 0.5 g) on cell biomass and trigonelline content of suspension cell culture of A.precatorius was studied. Suspension cell culture of A. precatorius was developed in a 100 mL conical flask containing 30 mL MS medium supplemented with 0.5 mg/L kinetin and 0.5 mg/L 2, 4- D. Results showed that 0.5 g inoculum had higher cell biomass with maximum biomass of 0.0795 g than 0.1 g inoculum that had biomass decrement throughout the culture. Furthermore, addition of 50 µM MeJa had multiplied trigonelline content to five times (6.62 mg/L) compared to the control (1.41 mg/L) for a period of 2 weeks. The correlation coefficient test indicated that cell biomass was not correlated with trigonelline content. Different increment of cell biomass with trigonelline suggested that trigonelline was not involved in regulating cell cycle of A. precatorius. In conclusion, 0.5 g inoculum size was a better size compared to 0.1 g inoculum suspension cell culture. The optimum trigonelline during cell growth of A.precatorius was also discovered.
format	Thesis
qualification_level	Master's degree
author	Ramly, Ahmad Lutfi
author_facet	Ramly, Ahmad Lutfi
author_sort	Ramly, Ahmad Lutfi
title	Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius
title_short	Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius
title_full	Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius
title_fullStr	Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius
title_full_unstemmed	Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius
title_sort	effect of methyl jasmonater on trigonelline content of suspension cell culture of abrus precatorius
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/77815/1/AhmadLutfiRamlyMFBME2016.pdf
_version_	1747817837765853184

Effect of methyl jasmonater on trigonelline content of suspension cell culture of Abrus Precatorius

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