Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy

A new online multi-stacking preconcentration technique based on the hybrid integration of field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in a battery-operated aqueous-based microchip capillary electrophoresis (MCE) device with a commerciall...

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Main Author:	Chong, Kah Chun
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/77892/1/ChongKahChunMFS2017.pdf
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spelling	my-utm-ep.778922018-07-23T05:46:08Z Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy 2017-06 Chong, Kah Chun QD Chemistry A new online multi-stacking preconcentration technique based on the hybrid integration of field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in a battery-operated aqueous-based microchip capillary electrophoresis (MCE) device with a commercially available double T-junction glass chip. Cationic analytes from two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte, and a sensitivity enhancement factor (SEF) of up to 217-fold was achieved. The developed approach was subsequently implemented in the MCE, coupled with contactless conductivity detection (C4D) in order to monitor the targeted antibiotic, namely vancomycin which was present in the human plasma samples. Moreover, the operation and parameters affecting the MSS, such as the BGE concentration, micelle concentration, focusing time, and methanol percentage in the sample solution were optimized and investigated. The multi-stacking and analysis time for vancomycin were 50 s and 250 s respectively, with the SEF of 83-fold as compared to the typical gated injection method. The detection limit of the method for the vancomycin spiked human plasma was 2.5 µg/mL, with intraday and interday repeatability (RSD) of 2.6% and 4.3%, respectively. The recoveries in the vancomycin spiked human plasma samples were 99.0 % –99.2 %. 2017-06 Thesis http://eprints.utm.my/id/eprint/77892/ http://eprints.utm.my/id/eprint/77892/1/ChongKahChunMFS2017.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:105133 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Chong, Kah Chun Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
description	A new online multi-stacking preconcentration technique based on the hybrid integration of field-enhanced sample injection (FESI) and micelle-to-solvent stacking (MSS) was developed and implemented in a battery-operated aqueous-based microchip capillary electrophoresis (MCE) device with a commercially available double T-junction glass chip. Cationic analytes from two sample reservoirs were injected under FESI conditions and subsequently focused by MSS within the sample loading channel. The proposed multi-stacking strategy was verified under a fluorescence microscope using Rhodamine 6G as the model analyte, and a sensitivity enhancement factor (SEF) of up to 217-fold was achieved. The developed approach was subsequently implemented in the MCE, coupled with contactless conductivity detection (C4D) in order to monitor the targeted antibiotic, namely vancomycin which was present in the human plasma samples. Moreover, the operation and parameters affecting the MSS, such as the BGE concentration, micelle concentration, focusing time, and methanol percentage in the sample solution were optimized and investigated. The multi-stacking and analysis time for vancomycin were 50 s and 250 s respectively, with the SEF of 83-fold as compared to the typical gated injection method. The detection limit of the method for the vancomycin spiked human plasma was 2.5 µg/mL, with intraday and interday repeatability (RSD) of 2.6% and 4.3%, respectively. The recoveries in the vancomycin spiked human plasma samples were 99.0 % –99.2 %.
format	Thesis
qualification_level	Master's degree
author	Chong, Kah Chun
author_facet	Chong, Kah Chun
author_sort	Chong, Kah Chun
title	Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
title_short	Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
title_full	Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
title_fullStr	Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
title_full_unstemmed	Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
title_sort	monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2017
url	http://eprints.utm.my/id/eprint/77892/1/ChongKahChunMFS2017.pdf
_version_	1747817856637075456

Monitoring of vancomycin in human plasma via portable microchip electrophoresis with contactless conductivity detector and multi-stacking strategy

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