Differential extraction of DNA types in semen mixtures from multiple male contributors

The analysis of samples obtained from sexual assault cases usually involve the separation of mixtures comprising cells from male and female contributors in order to produce the single profile of suspect and victim. However, specific research on the separation of mixed-male samples without the interf...

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Main Author:	Daud, Dharmeziyana
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/77894/1/DharmeziyanaDaudMFS2015SU.pdf
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spelling	my-utm-ep.778942018-07-23T06:00:16Z Differential extraction of DNA types in semen mixtures from multiple male contributors 2015-07 Daud, Dharmeziyana QD Chemistry The analysis of samples obtained from sexual assault cases usually involve the separation of mixtures comprising cells from male and female contributors in order to produce the single profile of suspect and victim. However, specific research on the separation of mixed-male samples without the interference from female cells is lacking. This study is necessary since certain cases of sexual assault involve male victim (sodomy cases) or multiple male suspects (gang-rape cases) and their effects to the pattern of separation should not be neglected. Differential extraction (DE) is the most commonly used method to separate the mixed sample as, theoretically, it is able to produce complete separation of mixtures from male and female individual. However, incomplete separation following DE is possible since the separation is affected by various factors. In this research, the pattern of sperm cells separation in male mixtures contributed by multiple numbers of sperm contributors with different proportion of major and minor contributors was studied. This research utilized semen specimens obtained from volunteering donors which were used to prepare four designed mixtures from multiple numbers of contributors in varying proportion of major and minor contributors in order to determine the effects of both factors to the pattern of cells separation by DE method. It was found that the sperm cells from mixtures contributed by as low as two individuals were successfully separated into both sperm cells (SC) and non-sperm cells (NSC) fraction. This verified that the male DNA profiles obtained from the NSC fraction may originate from the sperm cells and not the epithelial cells of male contributors. Mixture samples contributed by maximum of four males with various proportions of major and minor contributors revealed that the sperm cells from all contributors were separated into both SC and NSC fraction. However the ability to identify the major and minor contributors in both fractions were found to be dependent on different major and minor proportion of the original sample. 2015-07 Thesis http://eprints.utm.my/id/eprint/77894/ http://eprints.utm.my/id/eprint/77894/1/DharmeziyanaDaudMFS2015SU.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:94704 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Daud, Dharmeziyana Differential extraction of DNA types in semen mixtures from multiple male contributors
description	The analysis of samples obtained from sexual assault cases usually involve the separation of mixtures comprising cells from male and female contributors in order to produce the single profile of suspect and victim. However, specific research on the separation of mixed-male samples without the interference from female cells is lacking. This study is necessary since certain cases of sexual assault involve male victim (sodomy cases) or multiple male suspects (gang-rape cases) and their effects to the pattern of separation should not be neglected. Differential extraction (DE) is the most commonly used method to separate the mixed sample as, theoretically, it is able to produce complete separation of mixtures from male and female individual. However, incomplete separation following DE is possible since the separation is affected by various factors. In this research, the pattern of sperm cells separation in male mixtures contributed by multiple numbers of sperm contributors with different proportion of major and minor contributors was studied. This research utilized semen specimens obtained from volunteering donors which were used to prepare four designed mixtures from multiple numbers of contributors in varying proportion of major and minor contributors in order to determine the effects of both factors to the pattern of cells separation by DE method. It was found that the sperm cells from mixtures contributed by as low as two individuals were successfully separated into both sperm cells (SC) and non-sperm cells (NSC) fraction. This verified that the male DNA profiles obtained from the NSC fraction may originate from the sperm cells and not the epithelial cells of male contributors. Mixture samples contributed by maximum of four males with various proportions of major and minor contributors revealed that the sperm cells from all contributors were separated into both SC and NSC fraction. However the ability to identify the major and minor contributors in both fractions were found to be dependent on different major and minor proportion of the original sample.
format	Thesis
qualification_level	Master's degree
author	Daud, Dharmeziyana
author_facet	Daud, Dharmeziyana
author_sort	Daud, Dharmeziyana
title	Differential extraction of DNA types in semen mixtures from multiple male contributors
title_short	Differential extraction of DNA types in semen mixtures from multiple male contributors
title_full	Differential extraction of DNA types in semen mixtures from multiple male contributors
title_fullStr	Differential extraction of DNA types in semen mixtures from multiple male contributors
title_full_unstemmed	Differential extraction of DNA types in semen mixtures from multiple male contributors
title_sort	differential extraction of dna types in semen mixtures from multiple male contributors
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2015
url	http://eprints.utm.my/id/eprint/77894/1/DharmeziyanaDaudMFS2015SU.pdf
_version_	1747817857122566144

Differential extraction of DNA types in semen mixtures from multiple male contributors

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