Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)

In recent years, public interest on skincare cosmetics has shifted from synthetic to natural product due to adverse reactions of synthetic skincare cosmetics. In present study, α-mangostin from pericarp extract of Garcinia Mangostana Linn was evaluated for its in vitro antioxidant, antimicrobial and...

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Main Author:	Teng, Yi Siew
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/77957/1/TengYiSiewMFChE20171.pdf
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spelling	my-utm-ep.779572018-07-18T07:38:15Z Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.) 2017-08 Teng, Yi Siew TP Chemical technology In recent years, public interest on skincare cosmetics has shifted from synthetic to natural product due to adverse reactions of synthetic skincare cosmetics. In present study, α-mangostin from pericarp extract of Garcinia Mangostana Linn was evaluated for its in vitro antioxidant, antimicrobial and anti-aging properties. Determination of total antioxidant capacity was carried out by DPPH (2,2-diphenyl- 1-picrylhydrazyl) radical scavenging activity and ABTS (2,2-azino-bis (3- ethylbenzothiazoline-6-sulphonic acid)) assay. The antimicrobial activity was assessed using Minimum Inhibitory Concentration (MIC) test against: three selected bacteria strains Staphylococcus aureus (S.aureus), Propionibacterium acnes (P. acnes) and Pseudomonas aeruginosa (P. aeruginosa); and two fungi strains Trichophyton rubrum (T. rubrum) and Trichophyton mentagrophytes (T. mentagrophytes). Meanwhile, Bromide MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium) assay, collagen estimation assay and anti-collagenase assay were carried out to investigate the in vitro anti-aging effect of α-mangostin on human skin fibroblast cells. The α-mangostin exhibited high anti-oxidant capacity with IC50 (Half maximal inhibitory concentration) value of 0.0288 mg and 0.4637 mg, using ABTS assay and DPPH assay, respectively. The MIC values against test bacteria ranged between 0.156 mg/ml and 10 mg/ml whereas against fungi, MIC ranged from 2.5 mg/ml to 10 mg/ml. The following is the order of antimicrobial efficiency of α- mangostin on selected test microbes: S. aureus > P. acnes > T. rubrum > P. aeruginosa > T. mentagrophytes. Meanwhile, α-mangostin significantly restored the synthesis of pro-collagen on UVB (Ultraviolet B) irradiated human skin fibroblast in a dose dependent manner. The collagen content was increased by 3-fold upon treatment of 2 μg/ml α-mangostin. Extract of α-mangostin also showed good inhibitory effect on collagenase at 89.89 ±1.52%. Based on the outcomes, the recommended α-mangostin concentration to be formulated in cosmetic product is ranged from 1-2 μg/ml. 2017-08 Thesis http://eprints.utm.my/id/eprint/77957/ http://eprints.utm.my/id/eprint/77957/1/TengYiSiewMFChE20171.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:105147 masters Universiti Teknologi Malaysia, Faculty of Chemical and Energy Engineering Faculty of Chemical and Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Teng, Yi Siew Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
description	In recent years, public interest on skincare cosmetics has shifted from synthetic to natural product due to adverse reactions of synthetic skincare cosmetics. In present study, α-mangostin from pericarp extract of Garcinia Mangostana Linn was evaluated for its in vitro antioxidant, antimicrobial and anti-aging properties. Determination of total antioxidant capacity was carried out by DPPH (2,2-diphenyl- 1-picrylhydrazyl) radical scavenging activity and ABTS (2,2-azino-bis (3- ethylbenzothiazoline-6-sulphonic acid)) assay. The antimicrobial activity was assessed using Minimum Inhibitory Concentration (MIC) test against: three selected bacteria strains Staphylococcus aureus (S.aureus), Propionibacterium acnes (P. acnes) and Pseudomonas aeruginosa (P. aeruginosa); and two fungi strains Trichophyton rubrum (T. rubrum) and Trichophyton mentagrophytes (T. mentagrophytes). Meanwhile, Bromide MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium) assay, collagen estimation assay and anti-collagenase assay were carried out to investigate the in vitro anti-aging effect of α-mangostin on human skin fibroblast cells. The α-mangostin exhibited high anti-oxidant capacity with IC50 (Half maximal inhibitory concentration) value of 0.0288 mg and 0.4637 mg, using ABTS assay and DPPH assay, respectively. The MIC values against test bacteria ranged between 0.156 mg/ml and 10 mg/ml whereas against fungi, MIC ranged from 2.5 mg/ml to 10 mg/ml. The following is the order of antimicrobial efficiency of α- mangostin on selected test microbes: S. aureus > P. acnes > T. rubrum > P. aeruginosa > T. mentagrophytes. Meanwhile, α-mangostin significantly restored the synthesis of pro-collagen on UVB (Ultraviolet B) irradiated human skin fibroblast in a dose dependent manner. The collagen content was increased by 3-fold upon treatment of 2 μg/ml α-mangostin. Extract of α-mangostin also showed good inhibitory effect on collagenase at 89.89 ±1.52%. Based on the outcomes, the recommended α-mangostin concentration to be formulated in cosmetic product is ranged from 1-2 μg/ml.
format	Thesis
qualification_level	Master's degree
author	Teng, Yi Siew
author_facet	Teng, Yi Siew
author_sort	Teng, Yi Siew
title	Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
title_short	Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
title_full	Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
title_fullStr	Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
title_full_unstemmed	Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
title_sort	characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical and Energy Engineering
granting_department	Faculty of Chemical and Energy Engineering
publishDate	2017
url	http://eprints.utm.my/id/eprint/77957/1/TengYiSiewMFChE20171.pdf
_version_	1747817872313286656

Characterization of antioxidant, antimicrobial and antiaging properties of alpha-mangostin from mangosteen pericarp (garcinia mangostana linn.)

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