Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration

Pure iron (Fe) and its alloys have been recently emphasized as potential biodegradable metals due to their good mechanical properties that are close to those of stainless steel 316L. This research was focused more on the study of cell-material interaction and to analyze the effect of corrosion produ...

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التفاصيل البيبلوغرافية
المؤلف الرئيسي:	Mohd. Daud, Nurizzati
التنسيق:	أطروحة
اللغة:	English
منشور في:	2014
الموضوعات:	QH301 Biology
الوصول للمادة أونلاين:	http://eprints.utm.my/id/eprint/77992/1/NurizzatiMohdDaudMFBME20141.pdf
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spelling	my-utm-ep.779922018-07-18T07:50:01Z Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration 2014-03 Mohd. Daud, Nurizzati QH301 Biology Pure iron (Fe) and its alloys have been recently emphasized as potential biodegradable metals due to their good mechanical properties that are close to those of stainless steel 316L. This research was focused more on the study of cell-material interaction and to analyze the effect of corrosion product on cell behavior by performing degradation study. In this study, samples were prepared by coating hydroxyapatite (HA) and hydroxyapatite/poly (s-Caprolactone) (HA/PCL) onto porous iron using dip coating method. Biosafety and biofunctionality of the sample were evaluated by using human skin fibroblast (HSF) and mesenchymal stem (MSC) cells. Analysis by Inductively Coupled Plasma- Mass Spectrometry (ICP-MS) revealed that concentration of ion Fe was decreased in the medium containing HAcoated Fe. However, the weight loss of the sample is high compared to pure porous iron and HA/PCL-coated Fe. A positive cell response to the Fe ions was revealed during the first 21 days of the cell toxicity study using indirect method. After 21 days the HSF cell viability decreased due to acidic eluates and the increase of Fe ions concentration that promoted the formation of the reactive oxygen intermediates (ROI). From the results obtained, it showed that the HSF and MSC cells exhibited higher viability when in contact with the Fe-HA and Fe-PCL/HA than with the Fe specimens. However, there is a significant decrease (p<0.05) of cells when cultured on three different samples after 3 days of incubation. HA-coated porous Fe also provides support for attachment of the cells. Observation under Scanning Electron Microscope (SEM) reveals that the filopodia of the mesenchymal stem cells preferred to develop onto irregular surface of HA-coated Fe. This study provided evidences of a good cell-material interaction on the porous Fe that may confirm the feasibility of using porous biodegradable ferum as hard tissue scaffolds. 2014-03 Thesis http://eprints.utm.my/id/eprint/77992/ http://eprints.utm.my/id/eprint/77992/1/NurizzatiMohdDaudMFBME20141.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:90311 masters Universiti Teknologi Malaysia, Faculty of Built Environment Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Mohd. Daud, Nurizzati Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
description	Pure iron (Fe) and its alloys have been recently emphasized as potential biodegradable metals due to their good mechanical properties that are close to those of stainless steel 316L. This research was focused more on the study of cell-material interaction and to analyze the effect of corrosion product on cell behavior by performing degradation study. In this study, samples were prepared by coating hydroxyapatite (HA) and hydroxyapatite/poly (s-Caprolactone) (HA/PCL) onto porous iron using dip coating method. Biosafety and biofunctionality of the sample were evaluated by using human skin fibroblast (HSF) and mesenchymal stem (MSC) cells. Analysis by Inductively Coupled Plasma- Mass Spectrometry (ICP-MS) revealed that concentration of ion Fe was decreased in the medium containing HAcoated Fe. However, the weight loss of the sample is high compared to pure porous iron and HA/PCL-coated Fe. A positive cell response to the Fe ions was revealed during the first 21 days of the cell toxicity study using indirect method. After 21 days the HSF cell viability decreased due to acidic eluates and the increase of Fe ions concentration that promoted the formation of the reactive oxygen intermediates (ROI). From the results obtained, it showed that the HSF and MSC cells exhibited higher viability when in contact with the Fe-HA and Fe-PCL/HA than with the Fe specimens. However, there is a significant decrease (p<0.05) of cells when cultured on three different samples after 3 days of incubation. HA-coated porous Fe also provides support for attachment of the cells. Observation under Scanning Electron Microscope (SEM) reveals that the filopodia of the mesenchymal stem cells preferred to develop onto irregular surface of HA-coated Fe. This study provided evidences of a good cell-material interaction on the porous Fe that may confirm the feasibility of using porous biodegradable ferum as hard tissue scaffolds.
format	Thesis
qualification_level	Master's degree
author	Mohd. Daud, Nurizzati
author_facet	Mohd. Daud, Nurizzati
author_sort	Mohd. Daud, Nurizzati
title	Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_short	Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_full	Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_fullStr	Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_full_unstemmed	Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
title_sort	biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration
granting_institution	Universiti Teknologi Malaysia, Faculty of Built Environment
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2014
url	http://eprints.utm.my/id/eprint/77992/1/NurizzatiMohdDaudMFBME20141.pdf
_version_	1747817880834015232

Biological assessment of uncoated and hydroxyapatitecoated ferum as biodegradable scaffold for hard tissue regeneration

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