The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay

Angiogenesis, the formation of new vascular network from the pre-existing vasculature, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineer...

Full description

Saved in:

Bibliographic Details
Main Author:	Ishak, Siti Amirah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	QH301 Biology
Online Access:	http://eprints.utm.my/id/eprint/78026/1/SitiAmirahIshakMFBME20151.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.78026
record_format	uketd_dc
spelling	my-utm-ep.780262018-07-23T05:33:05Z The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay 2015-01 Ishak, Siti Amirah QH301 Biology Angiogenesis, the formation of new vascular network from the pre-existing vasculature, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineered tissue constructs, mimicking a functional blood vessel in vivo. Besides, the formation of new blood vessels depends strongly on the extracellular matrix such as fibrin gel. In this study, we investigate the effect of four different ratio of fibrinogen (mg/mL) to thrombin (Units/mL) composition (i.e., 2:1, 1:2, 1:8, 8:1), on the physical properties of fibrin gel and the role of fibrin gel for angiogenesis assay with HSF. Some properties of fibrin gel such as clotting time, water uptake property, turbidity and microstructure observation was studied. Higher concentration of thrombin (i.e., 8:1 mg/U) will yields a shorter clotting time of the fibrin gel when compared to the lower concentration of thrombin (i.e 1:8 mg/U). Fibrin gel with higher thrombin and lower fibrinogen concentration (i.e., 1:8 mg/U) are not stable enough to withstand the amount of PBS subjected to it hence it cannot absorb water well compared to other fibrin gel concentration. Meanwhile, at higher concentration of thrombin, the turbidity study shows the lowest absorbance compared to other samples. Different ratio of fibrinogen to thrombin may affect the microstructure of the fibrin gel produced. Microstructure observation proved that fibrin gel produced in this study shows the fibrous structure. The last part of this experiment also proved that fibrin gel with ratio of 2:1 mg/U of fibrinogen to thrombin reveal the best tube-like structure which has the highest number of nucleus as well as highest number of tube-like structure formed after 48 hours. 2015-01 Thesis http://eprints.utm.my/id/eprint/78026/ http://eprints.utm.my/id/eprint/78026/1/SitiAmirahIshakMFBME20151.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:90309 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Ishak, Siti Amirah The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
description	Angiogenesis, the formation of new vascular network from the pre-existing vasculature, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineered tissue constructs, mimicking a functional blood vessel in vivo. Besides, the formation of new blood vessels depends strongly on the extracellular matrix such as fibrin gel. In this study, we investigate the effect of four different ratio of fibrinogen (mg/mL) to thrombin (Units/mL) composition (i.e., 2:1, 1:2, 1:8, 8:1), on the physical properties of fibrin gel and the role of fibrin gel for angiogenesis assay with HSF. Some properties of fibrin gel such as clotting time, water uptake property, turbidity and microstructure observation was studied. Higher concentration of thrombin (i.e., 8:1 mg/U) will yields a shorter clotting time of the fibrin gel when compared to the lower concentration of thrombin (i.e 1:8 mg/U). Fibrin gel with higher thrombin and lower fibrinogen concentration (i.e., 1:8 mg/U) are not stable enough to withstand the amount of PBS subjected to it hence it cannot absorb water well compared to other fibrin gel concentration. Meanwhile, at higher concentration of thrombin, the turbidity study shows the lowest absorbance compared to other samples. Different ratio of fibrinogen to thrombin may affect the microstructure of the fibrin gel produced. Microstructure observation proved that fibrin gel produced in this study shows the fibrous structure. The last part of this experiment also proved that fibrin gel with ratio of 2:1 mg/U of fibrinogen to thrombin reveal the best tube-like structure which has the highest number of nucleus as well as highest number of tube-like structure formed after 48 hours.
format	Thesis
qualification_level	Master's degree
author	Ishak, Siti Amirah
author_facet	Ishak, Siti Amirah
author_sort	Ishak, Siti Amirah
title	The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_short	The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_full	The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_fullStr	The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_full_unstemmed	The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_sort	effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/78026/1/SitiAmirahIshakMFBME20151.pdf
_version_	1747817889143980032

The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay

Similar Items