The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay

Angiogenesis, the formation of new vascular network from the pre-existing vasculature, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineer...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Ishak, Siti Amirah
التنسيق: أطروحة
اللغة:English
منشور في: 2015
الموضوعات:
الوصول للمادة أونلاين:http://eprints.utm.my/id/eprint/78026/1/SitiAmirahIshakMFBME20151.pdf
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spelling my-utm-ep.780262018-07-23T05:33:05Z The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay 2015-01 Ishak, Siti Amirah QH301 Biology Angiogenesis, the formation of new vascular network from the pre-existing vasculature, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineered tissue constructs, mimicking a functional blood vessel in vivo. Besides, the formation of new blood vessels depends strongly on the extracellular matrix such as fibrin gel. In this study, we investigate the effect of four different ratio of fibrinogen (mg/mL) to thrombin (Units/mL) composition (i.e., 2:1, 1:2, 1:8, 8:1), on the physical properties of fibrin gel and the role of fibrin gel for angiogenesis assay with HSF. Some properties of fibrin gel such as clotting time, water uptake property, turbidity and microstructure observation was studied. Higher concentration of thrombin (i.e., 8:1 mg/U) will yields a shorter clotting time of the fibrin gel when compared to the lower concentration of thrombin (i.e 1:8 mg/U). Fibrin gel with higher thrombin and lower fibrinogen concentration (i.e., 1:8 mg/U) are not stable enough to withstand the amount of PBS subjected to it hence it cannot absorb water well compared to other fibrin gel concentration. Meanwhile, at higher concentration of thrombin, the turbidity study shows the lowest absorbance compared to other samples. Different ratio of fibrinogen to thrombin may affect the microstructure of the fibrin gel produced. Microstructure observation proved that fibrin gel produced in this study shows the fibrous structure. The last part of this experiment also proved that fibrin gel with ratio of 2:1 mg/U of fibrinogen to thrombin reveal the best tube-like structure which has the highest number of nucleus as well as highest number of tube-like structure formed after 48 hours. 2015-01 Thesis http://eprints.utm.my/id/eprint/78026/ http://eprints.utm.my/id/eprint/78026/1/SitiAmirahIshakMFBME20151.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:90309 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic QH301 Biology
spellingShingle QH301 Biology
Ishak, Siti Amirah
The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
description Angiogenesis, the formation of new vascular network from the pre-existing vasculature, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineered tissue constructs, mimicking a functional blood vessel in vivo. Besides, the formation of new blood vessels depends strongly on the extracellular matrix such as fibrin gel. In this study, we investigate the effect of four different ratio of fibrinogen (mg/mL) to thrombin (Units/mL) composition (i.e., 2:1, 1:2, 1:8, 8:1), on the physical properties of fibrin gel and the role of fibrin gel for angiogenesis assay with HSF. Some properties of fibrin gel such as clotting time, water uptake property, turbidity and microstructure observation was studied. Higher concentration of thrombin (i.e., 8:1 mg/U) will yields a shorter clotting time of the fibrin gel when compared to the lower concentration of thrombin (i.e 1:8 mg/U). Fibrin gel with higher thrombin and lower fibrinogen concentration (i.e., 1:8 mg/U) are not stable enough to withstand the amount of PBS subjected to it hence it cannot absorb water well compared to other fibrin gel concentration. Meanwhile, at higher concentration of thrombin, the turbidity study shows the lowest absorbance compared to other samples. Different ratio of fibrinogen to thrombin may affect the microstructure of the fibrin gel produced. Microstructure observation proved that fibrin gel produced in this study shows the fibrous structure. The last part of this experiment also proved that fibrin gel with ratio of 2:1 mg/U of fibrinogen to thrombin reveal the best tube-like structure which has the highest number of nucleus as well as highest number of tube-like structure formed after 48 hours.
format Thesis
qualification_level Master's degree
author Ishak, Siti Amirah
author_facet Ishak, Siti Amirah
author_sort Ishak, Siti Amirah
title The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_short The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_full The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_fullStr The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_full_unstemmed The effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
title_sort effect of fibrin extracellular matrix properties for the in vitro angiogenesis assay
granting_institution Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department Faculty of Biosciences and Medical Engineering
publishDate 2015
url http://eprints.utm.my/id/eprint/78026/1/SitiAmirahIshakMFBME20151.pdf
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