Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities

Swietenia mahagoni is broadly used in traditional remedies. It contains several bioactive compounds such as ß-sitosterol. The aim of this study is to investigate the effect of supercritical carbon dioxide (SC-CO2) extraction conditions (pressure and temperature) on oil yield and ß-sitosterol content...

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Main Author:	Md. Norodin, Nur Salsabila
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/78375/1/NurSalsabilaMdMFChE2016.pdf
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spelling	my-utm-ep.783752018-08-26T04:56:12Z Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities 2016-12 Md. Norodin, Nur Salsabila TP Chemical technology Swietenia mahagoni is broadly used in traditional remedies. It contains several bioactive compounds such as ß-sitosterol. The aim of this study is to investigate the effect of supercritical carbon dioxide (SC-CO2) extraction conditions (pressure and temperature) on oil yield and ß-sitosterol content from switenia mahagoni seeds and to analyze the anti-diabetic activities of switenia mahagoni seeds extract. Swietenia mahagoni seeds extracts were obtained by SC-CO2 extraction at pressures of 20-30 MPa and temperatures of 40-60 °C. The optimization process was done by a three-level factorial design with a response surface methodology using Statistica software 7.0 (StatSoft, EUA). Quantification of ß-sitosterol content was performed by using a high performance liquid chromatography conducted on C18 reversed phase Kinetex Biphenyl column using methanol: acetonitrile (60:40 v/v) as mobile phase. The oil yields at different conditions obtained were analyzed with a-glucosidase and a-amylase inhibition assays. One way analysis was used for statistical significance by using Statistica software 7.0 (StatSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software. The experimental data of SC-CO2 extraction obtained were fitted to a second-order polynomial model and the obtained oil yields were between 1.5-14.5%, while ß- sitosterol content obtained were 3.1-9.2 mg/g. The highest inhibition of a- glucosidase and a-amylase enzymes were 98.4% ± 0.2 and 34.9% ± 1.2, respectively. The best conditions within the studied ranges were 30 MPa and 40 °C to extract ß- sitosterol in the highest amount. All conditions for extraction of swietenia mahagoni seeds were potent to a-glucosidase inhibitory assay but showed a weak inhibition toward a-amylase inhibitory assay. These findings implied that swietenia mahagoni seeds extract containing ß-sitosterol, could be an effective natural anti-diabetic agent. 2016-12 Thesis http://eprints.utm.my/id/eprint/78375/ http://eprints.utm.my/id/eprint/78375/1/NurSalsabilaMdMFChE2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:105173 masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Md. Norodin, Nur Salsabila Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities
description	Swietenia mahagoni is broadly used in traditional remedies. It contains several bioactive compounds such as ß-sitosterol. The aim of this study is to investigate the effect of supercritical carbon dioxide (SC-CO2) extraction conditions (pressure and temperature) on oil yield and ß-sitosterol content from switenia mahagoni seeds and to analyze the anti-diabetic activities of switenia mahagoni seeds extract. Swietenia mahagoni seeds extracts were obtained by SC-CO2 extraction at pressures of 20-30 MPa and temperatures of 40-60 °C. The optimization process was done by a three-level factorial design with a response surface methodology using Statistica software 7.0 (StatSoft, EUA). Quantification of ß-sitosterol content was performed by using a high performance liquid chromatography conducted on C18 reversed phase Kinetex Biphenyl column using methanol: acetonitrile (60:40 v/v) as mobile phase. The oil yields at different conditions obtained were analyzed with a-glucosidase and a-amylase inhibition assays. One way analysis was used for statistical significance by using Statistica software 7.0 (StatSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software. The experimental data of SC-CO2 extraction obtained were fitted to a second-order polynomial model and the obtained oil yields were between 1.5-14.5%, while ß- sitosterol content obtained were 3.1-9.2 mg/g. The highest inhibition of a- glucosidase and a-amylase enzymes were 98.4% ± 0.2 and 34.9% ± 1.2, respectively. The best conditions within the studied ranges were 30 MPa and 40 °C to extract ß- sitosterol in the highest amount. All conditions for extraction of swietenia mahagoni seeds were potent to a-glucosidase inhibitory assay but showed a weak inhibition toward a-amylase inhibitory assay. These findings implied that swietenia mahagoni seeds extract containing ß-sitosterol, could be an effective natural anti-diabetic agent.
format	Thesis
qualification_level	Master's degree
author	Md. Norodin, Nur Salsabila
author_facet	Md. Norodin, Nur Salsabila
author_sort	Md. Norodin, Nur Salsabila
title	Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities
title_short	Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities
title_full	Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities
title_fullStr	Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities
title_full_unstemmed	Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities
title_sort	optimization of β-sitosterol from swietenia mahagoni seeds extract using supercritical carbon dioxide (sc-co2) extraction and its anti-diabetic activities
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/78375/1/NurSalsabilaMdMFChE2016.pdf
_version_	1747817973882552320

Optimization of β-sitosterol from SWIETENIA MAHAGONI seeds extract using supercritical carbon dioxide (SC-CO2) extraction and its anti-diabetic activities

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