Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)

A laboratory scale temporary immersion bioreactor or also known as TIB has been developed in this study by applying the principle of temporary immersion system due to lacking of TIB development for local pineapple Sarawak cultivar production and higher production cost needed by using semi-solid cult...

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Main Author:	Ramli, Amiladziffar
Format:	Thesis
Language:	English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/78474/1/AmiladziffarRamliMFBME2018.pdf
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spelling	my-utm-ep.784742018-08-26T11:56:27Z Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.) 2018-03 Ramli, Amiladziffar TP Chemical technology A laboratory scale temporary immersion bioreactor or also known as TIB has been developed in this study by applying the principle of temporary immersion system due to lacking of TIB development for local pineapple Sarawak cultivar production and higher production cost needed by using semi-solid culture system. The effect of immersion time on shoot production frequency using a modified temporary immersion bioreactor was determined and the shoot induction efficiency of TIB and semi-solid system was also evaluated. The modified TIB was operated by adjusting the time for controlled solenoid valve to allow the transfer of MS liquid medium from medium container to the culture container. Different immersion time was tested by setting up the timer to 3 min/2h and 15 min/2h throughout the three weeks culture period. Results showed that 100% shoot regenerated with maximum number of shoots produced per explant (3 ± 0.58 shoots) when cultured in the TIB with 3 min/2h immersion time. Meanwhile, out of the two culture systems, the shoot regeneration efficiency in TIB managed to produce 3 ± 0.58 shoots within three weeks whereas the semi-solid system required six weeks to produce similar amounts of shoots (6 ± 1.16) which reduced shoot production time by half compared to the semi-solid system. From this study, it is suggested that modified TIB has promoted shoot regeneration frequency thus enhancing pineapple plantlets productivity. 2018-03 Thesis http://eprints.utm.my/id/eprint/78474/ http://eprints.utm.my/id/eprint/78474/1/AmiladziffarRamliMFBME2018.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:110580 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ramli, Amiladziffar Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)
description	A laboratory scale temporary immersion bioreactor or also known as TIB has been developed in this study by applying the principle of temporary immersion system due to lacking of TIB development for local pineapple Sarawak cultivar production and higher production cost needed by using semi-solid culture system. The effect of immersion time on shoot production frequency using a modified temporary immersion bioreactor was determined and the shoot induction efficiency of TIB and semi-solid system was also evaluated. The modified TIB was operated by adjusting the time for controlled solenoid valve to allow the transfer of MS liquid medium from medium container to the culture container. Different immersion time was tested by setting up the timer to 3 min/2h and 15 min/2h throughout the three weeks culture period. Results showed that 100% shoot regenerated with maximum number of shoots produced per explant (3 ± 0.58 shoots) when cultured in the TIB with 3 min/2h immersion time. Meanwhile, out of the two culture systems, the shoot regeneration efficiency in TIB managed to produce 3 ± 0.58 shoots within three weeks whereas the semi-solid system required six weeks to produce similar amounts of shoots (6 ± 1.16) which reduced shoot production time by half compared to the semi-solid system. From this study, it is suggested that modified TIB has promoted shoot regeneration frequency thus enhancing pineapple plantlets productivity.
format	Thesis
qualification_level	Master's degree
author	Ramli, Amiladziffar
author_facet	Ramli, Amiladziffar
author_sort	Ramli, Amiladziffar
title	Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)
title_short	Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)
title_full	Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)
title_fullStr	Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)
title_full_unstemmed	Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)
title_sort	low cost temporary immersion bioreactor for micropropagation of local pineapple (ananas comosus l.)
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2018
url	http://eprints.utm.my/id/eprint/78474/1/AmiladziffarRamliMFBME2018.pdf
_version_	1747817993489874944

Low cost temporary immersion bioreactor for micropropagation of local pineapple (Ananas comosus L.)

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