Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes

Biomaterials based scaffolds or membranes fabricated from electrospinning with suitable properties are highly desired in tissue engineering. Blending o f natural polymer with synthetic polymer allows the modulation o f properties to produce membranes for tissue engineering. Recently, conductive poly...

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Main Author:	Chang, Hui Chung
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/78539/1/ChangHuiChungMFBME2016.pdf
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id	my-utm-ep.78539
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spelling	my-utm-ep.785392018-08-29T07:31:49Z Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes 2016-05 Chang, Hui Chung TP Chemical technology Biomaterials based scaffolds or membranes fabricated from electrospinning with suitable properties are highly desired in tissue engineering. Blending o f natural polymer with synthetic polymer allows the modulation o f properties to produce membranes for tissue engineering. Recently, conductive polymers have gained great attention in research due to their conductive properties, which can stimulate tissue regeneration. In this study, composite membrane was fabricated by blending a synthetic polymer, polylactic acid (PLA) and a natural polymer, poly(3- hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using electrospinning technique. PLA/PHBV electrospun membranes were dipped into PEDOT:PSS solution to prepare conductive membranes. It was observed that electrospinning of 20 % (w/v) PLA/PHBV with the weight ratio of 50:50 in chloroform solvent produced the most uniform fibers with no beads. The coated and uncoated membranes were evaluated using several techniques, including scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), water contact angle (WCA), attenuated total reflectance (ATR), and atomic force microscopy (AFM). The measured electrical conductivity of the 30 % PEDOT:PSS coated PLA/PHBV was 1.45 pS/m. Also, the surface roughness and wettability of the PEDOT:PSS coated PLA/PHBV membranes were greater than the uncoated membranes. Based on the results of the cells viability of human skin fibroblast (HSF) using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell attachment and cell proliferation, the conductive PEDOT:PSS-coated PLA/PHBV membranes were found to be more favorable for tissue engineering application than the uncoated membranes. Antibacterial evaluation also showed that tetracycline hydrochloride (TCH)-coated membrane possess antibacterial properties. In conclusion, conductive PEDOT:PSS coated membrane that has the potential to be used in tissue engineering application was successfully fabricated and characterized. 2016-05 Thesis http://eprints.utm.my/id/eprint/78539/ http://eprints.utm.my/id/eprint/78539/1/ChangHuiChungMFBME2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:110588 phd doctoral Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Chang, Hui Chung Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
description	Biomaterials based scaffolds or membranes fabricated from electrospinning with suitable properties are highly desired in tissue engineering. Blending o f natural polymer with synthetic polymer allows the modulation o f properties to produce membranes for tissue engineering. Recently, conductive polymers have gained great attention in research due to their conductive properties, which can stimulate tissue regeneration. In this study, composite membrane was fabricated by blending a synthetic polymer, polylactic acid (PLA) and a natural polymer, poly(3- hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) using electrospinning technique. PLA/PHBV electrospun membranes were dipped into PEDOT:PSS solution to prepare conductive membranes. It was observed that electrospinning of 20 % (w/v) PLA/PHBV with the weight ratio of 50:50 in chloroform solvent produced the most uniform fibers with no beads. The coated and uncoated membranes were evaluated using several techniques, including scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), water contact angle (WCA), attenuated total reflectance (ATR), and atomic force microscopy (AFM). The measured electrical conductivity of the 30 % PEDOT:PSS coated PLA/PHBV was 1.45 pS/m. Also, the surface roughness and wettability of the PEDOT:PSS coated PLA/PHBV membranes were greater than the uncoated membranes. Based on the results of the cells viability of human skin fibroblast (HSF) using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell attachment and cell proliferation, the conductive PEDOT:PSS-coated PLA/PHBV membranes were found to be more favorable for tissue engineering application than the uncoated membranes. Antibacterial evaluation also showed that tetracycline hydrochloride (TCH)-coated membrane possess antibacterial properties. In conclusion, conductive PEDOT:PSS coated membrane that has the potential to be used in tissue engineering application was successfully fabricated and characterized.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Chang, Hui Chung
author_facet	Chang, Hui Chung
author_sort	Chang, Hui Chung
title	Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
title_short	Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
title_full	Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
title_fullStr	Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
title_full_unstemmed	Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
title_sort	synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/78539/1/ChangHuiChungMFBME2016.pdf
_version_	1747818010029064192

Synthesis and characterization of poly(3,4- ethylenedioxythiophene)-poly(styrenesulfonate) coated polylactide/poly(3-hydroxybutyrate-co-3- hydroxyvalerate) electrospun membranes

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