Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate

The rising gap between the demand for fossil fuel-based energy and insufficient energy supply has led to the vast investigation of potential alternative clean energy, biohydrogen. In this study, starch utilizing bacteria Enterococcus sp. NF6 was isolated from cow manure; the effective starch utiliza...

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Main Author:	N. Baniear Salvam, Dharsigah
Format:	Thesis
Language:	English
Published:	2017
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/78560/1/DharsigahNBaniearSalvamMFBME2017.pdf
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spelling	my-utm-ep.785602018-08-29T07:31:51Z Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate 2017-07 N. Baniear Salvam, Dharsigah TP Chemical technology The rising gap between the demand for fossil fuel-based energy and insufficient energy supply has led to the vast investigation of potential alternative clean energy, biohydrogen. In this study, starch utilizing bacteria Enterococcus sp. NF6 was isolated from cow manure; the effective starch utilization and biohydrogen production of the biocatalyst were investigated. To investigate the optimum conditions for biohydrogen production, batch fermentations were conducted by optimization of physicochemical parameters using one factor at a time (OFAT) method using Modified Reinforced Clostridial Media (MRCM) as fermentation media. Batch fermentations were carried out at several temperatures (30°C, 37°C & 45°C), initial medium pH (5.5, 7.0, 8.0 & 9.0) and initial substrate concentration of (0.5 g/L, 1 g/L & 3g/L). The highest biohydrogen production obtained at pH 5.5, incubation temperature of 37°C, and initial substrate concentration of 1g/L. These conditions, the biohydrogen production rate is 0.23 mL/H, with 100% of substrate utilization, 0.0790 h-1 of specific growth rate and maximum biohydrogen production of 1.85 mL. The outcome suggests the potential use of Enterococcus sp. NF6 for biohydrogen production in a single culture system. 2017-07 Thesis http://eprints.utm.my/id/eprint/78560/ http://eprints.utm.my/id/eprint/78560/1/DharsigahNBaniearSalvamMFBME2017.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:108143 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology N. Baniear Salvam, Dharsigah Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
description	The rising gap between the demand for fossil fuel-based energy and insufficient energy supply has led to the vast investigation of potential alternative clean energy, biohydrogen. In this study, starch utilizing bacteria Enterococcus sp. NF6 was isolated from cow manure; the effective starch utilization and biohydrogen production of the biocatalyst were investigated. To investigate the optimum conditions for biohydrogen production, batch fermentations were conducted by optimization of physicochemical parameters using one factor at a time (OFAT) method using Modified Reinforced Clostridial Media (MRCM) as fermentation media. Batch fermentations were carried out at several temperatures (30°C, 37°C & 45°C), initial medium pH (5.5, 7.0, 8.0 & 9.0) and initial substrate concentration of (0.5 g/L, 1 g/L & 3g/L). The highest biohydrogen production obtained at pH 5.5, incubation temperature of 37°C, and initial substrate concentration of 1g/L. These conditions, the biohydrogen production rate is 0.23 mL/H, with 100% of substrate utilization, 0.0790 h-1 of specific growth rate and maximum biohydrogen production of 1.85 mL. The outcome suggests the potential use of Enterococcus sp. NF6 for biohydrogen production in a single culture system.
format	Thesis
qualification_level	Master's degree
author	N. Baniear Salvam, Dharsigah
author_facet	N. Baniear Salvam, Dharsigah
author_sort	N. Baniear Salvam, Dharsigah
title	Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
title_short	Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
title_full	Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
title_fullStr	Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
title_full_unstemmed	Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate
title_sort	optimization of biohydrogen production by enterococcus sp. nf6 using starch as substrate
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2017
url	http://eprints.utm.my/id/eprint/78560/1/DharsigahNBaniearSalvamMFBME2017.pdf
_version_	1747818015265652736

Optimization of biohydrogen production by Enterococcus SP. NF6 using starch as substrate

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