Novel biodegradable patch for atrial septal defect closure

Since there are still occuring severe complications due to atrial septal defect (ASD) occluder, the development of a suitable ASD device is still in procuring. In the last years researcher focused on biodegradable devices. Accordingly, in this research a degradable occluding patch has been developed...

Full description

Saved in:

Bibliographic Details
Main Author:	Kaiser, Eva
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH301 Biology
Online Access:	http://eprints.utm.my/id/eprint/78562/1/EvaChristinaKaiserMFBME2017.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.78562
record_format	uketd_dc
spelling	my-utm-ep.785622018-08-29T07:31:54Z Novel biodegradable patch for atrial septal defect closure 2016-09 Kaiser, Eva QH301 Biology Since there are still occuring severe complications due to atrial septal defect (ASD) occluder, the development of a suitable ASD device is still in procuring. In the last years researcher focused on biodegradable devices. Accordingly, in this research a degradable occluding patch has been developed, to analyse the ability to solve existing complications. Therefore biodegradable patches were electrospun using medical grade polyurethane (PU) added with bioactive agents, chitosan nanoparticles (ChNP), collagen (Co) and heparin (Hep). The control patch was pure PU. ChNP were added to improve the mechanical properties and bolster the PU.The collagen is expected to provide an extracellular matrix improving cell adhesion and cell growth, serving as a biological sealant of the ASD. FTIR showed characteristic vibrations of active constituents and changes in the absorbance due to the ingredients. The contact angle analysis demonstrated no significance comparing control and composite patches. The mean values for the PU, PUChCo and coated PuChCo were found as 84.23° ± 1.06, 87.62° ± 3.73 and 90.42° ± 1.41 (p < 0.05). Moreover, the structure of the electrospun composite fibres were meticulously displayed through scanning electron microscopy. The decrease in nanofibre diameter (PU: 0445.7 nm to PUChCo: 0275.0 nm) between control and composite is due to a change of viscosity of the spinning solution after adding Co. The haemocompatible properties of the patches (PU, PUChCo, PUChCoHep) were inferred through in vitro tests, e.g. activated partial thromboplastin time (72.92 s, 70.77 s,103.33 s), prothrombin time (25.73 s, 29.4 s, 35.67 s) and haemolysis assay (3.64 %, 2.39 %, 2.12 %). In conclusion, the developed patch was observed to show desirable properties for an application in an ASD occlusion device. 2016-09 Thesis http://eprints.utm.my/id/eprint/78562/ http://eprints.utm.my/id/eprint/78562/1/EvaChristinaKaiserMFBME2017.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:110788 phd doctoral Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH301 Biology
spellingShingle	QH301 Biology Kaiser, Eva Novel biodegradable patch for atrial septal defect closure
description	Since there are still occuring severe complications due to atrial septal defect (ASD) occluder, the development of a suitable ASD device is still in procuring. In the last years researcher focused on biodegradable devices. Accordingly, in this research a degradable occluding patch has been developed, to analyse the ability to solve existing complications. Therefore biodegradable patches were electrospun using medical grade polyurethane (PU) added with bioactive agents, chitosan nanoparticles (ChNP), collagen (Co) and heparin (Hep). The control patch was pure PU. ChNP were added to improve the mechanical properties and bolster the PU.The collagen is expected to provide an extracellular matrix improving cell adhesion and cell growth, serving as a biological sealant of the ASD. FTIR showed characteristic vibrations of active constituents and changes in the absorbance due to the ingredients. The contact angle analysis demonstrated no significance comparing control and composite patches. The mean values for the PU, PUChCo and coated PuChCo were found as 84.23° ± 1.06, 87.62° ± 3.73 and 90.42° ± 1.41 (p < 0.05). Moreover, the structure of the electrospun composite fibres were meticulously displayed through scanning electron microscopy. The decrease in nanofibre diameter (PU: 0445.7 nm to PUChCo: 0275.0 nm) between control and composite is due to a change of viscosity of the spinning solution after adding Co. The haemocompatible properties of the patches (PU, PUChCo, PUChCoHep) were inferred through in vitro tests, e.g. activated partial thromboplastin time (72.92 s, 70.77 s,103.33 s), prothrombin time (25.73 s, 29.4 s, 35.67 s) and haemolysis assay (3.64 %, 2.39 %, 2.12 %). In conclusion, the developed patch was observed to show desirable properties for an application in an ASD occlusion device.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Kaiser, Eva
author_facet	Kaiser, Eva
author_sort	Kaiser, Eva
title	Novel biodegradable patch for atrial septal defect closure
title_short	Novel biodegradable patch for atrial septal defect closure
title_full	Novel biodegradable patch for atrial septal defect closure
title_fullStr	Novel biodegradable patch for atrial septal defect closure
title_full_unstemmed	Novel biodegradable patch for atrial septal defect closure
title_sort	novel biodegradable patch for atrial septal defect closure
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/78562/1/EvaChristinaKaiserMFBME2017.pdf
_version_	1747818015773163520

Novel biodegradable patch for atrial septal defect closure

Similar Items