Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads

Palm oil mill effluent (POME) is an agro-industrial wastewater from palm oil processing. It is characterized by presence high organic compounds that cause serious air, soil and water pollutions. Both large and laboratory scale technologies are used to solve the problems that evolved from the dischar...

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Main Author:	Matinja, Idris Adamu
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/78595/1/IdrisAdamuMatinjaMFBME2016.pdf
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spelling	my-utm-ep.785952018-08-29T07:33:57Z Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads 2016-01 Matinja, Idris Adamu TP Chemical technology Palm oil mill effluent (POME) is an agro-industrial wastewater from palm oil processing. It is characterized by presence high organic compounds that cause serious air, soil and water pollutions. Both large and laboratory scale technologies are used to solve the problems that evolved from the discharge of POME. Previously, biodiesel was obtained from POME using immobilized Candida rugosa lipase in Polyvinyl alcohol (PVA) alginate-sulfate beads, which prior that study, was seen as a good substrate for biodiesel production due to its high amount of oil and grease. In this study, biodiesel production from POME using immobilized Candida rugosa lipase in PVA-alginate-sulfate beads was optimized using Box-Behnken design (BBD) of response surface methodology (RSM). Four parameters responsible for biodiesel production from POME were adopted from the previous study; these are methanol/POME ratio, reaction time, weight of the immobilized beads and agitation speed. The highest conversion (91%) and biodiesel yield of oleic methyl esters (65%) and palmitic methyl esters (45%) were obtained at the following optimum conditions; agitation speed (300rpm), POME/methanol ratio (1:6), incubation period (5hours) and weight of the immobilized beads weight (2g). Michaelis-Menten kinetic parameters of the immobilized and free lipase were determined through the hydrolysis of olive oil. Lineweaver-Burk plot indicated the corresponding values of Km and Vmax were 0.0506 g/ml and 21.09 U/ml for free lipase and 0.0686 g/ml and 15.95 U/ml for immobilized lipase. The important fuel properties of the biodiesel such as flash point, kinematic viscosity, water and sediment and copper strip corrosion were evaluated according to the American Society for Testing of Materials (ASTM D6751) and European Standard (EN 14214) and were found to be in good agreement with the standard quality and specification. 2016-01 Thesis http://eprints.utm.my/id/eprint/78595/ http://eprints.utm.my/id/eprint/78595/1/IdrisAdamuMatinjaMFBME2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:109675 masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Matinja, Idris Adamu Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads
description	Palm oil mill effluent (POME) is an agro-industrial wastewater from palm oil processing. It is characterized by presence high organic compounds that cause serious air, soil and water pollutions. Both large and laboratory scale technologies are used to solve the problems that evolved from the discharge of POME. Previously, biodiesel was obtained from POME using immobilized Candida rugosa lipase in Polyvinyl alcohol (PVA) alginate-sulfate beads, which prior that study, was seen as a good substrate for biodiesel production due to its high amount of oil and grease. In this study, biodiesel production from POME using immobilized Candida rugosa lipase in PVA-alginate-sulfate beads was optimized using Box-Behnken design (BBD) of response surface methodology (RSM). Four parameters responsible for biodiesel production from POME were adopted from the previous study; these are methanol/POME ratio, reaction time, weight of the immobilized beads and agitation speed. The highest conversion (91%) and biodiesel yield of oleic methyl esters (65%) and palmitic methyl esters (45%) were obtained at the following optimum conditions; agitation speed (300rpm), POME/methanol ratio (1:6), incubation period (5hours) and weight of the immobilized beads weight (2g). Michaelis-Menten kinetic parameters of the immobilized and free lipase were determined through the hydrolysis of olive oil. Lineweaver-Burk plot indicated the corresponding values of Km and Vmax were 0.0506 g/ml and 21.09 U/ml for free lipase and 0.0686 g/ml and 15.95 U/ml for immobilized lipase. The important fuel properties of the biodiesel such as flash point, kinematic viscosity, water and sediment and copper strip corrosion were evaluated according to the American Society for Testing of Materials (ASTM D6751) and European Standard (EN 14214) and were found to be in good agreement with the standard quality and specification.
format	Thesis
qualification_level	Master's degree
author	Matinja, Idris Adamu
author_facet	Matinja, Idris Adamu
author_sort	Matinja, Idris Adamu
title	Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads
title_short	Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads
title_full	Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads
title_fullStr	Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads
title_full_unstemmed	Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads
title_sort	optimization of biodiesel production from pome using lipase immobilized in pva-alginate-sulfate beads
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/78595/1/IdrisAdamuMatinjaMFBME2016.pdf
_version_	1747818024047476736

Optimization of biodiesel production from POME using lipase immobilized in PVA-alginate-sulfate beads

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