Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy

Terahertz and infrared spectroscopy are effective analytical spectroscopic techniques to identify and study the conformation and molecular interaction of the biomolecules. It has a huge potential in cancer diagnosis because these spectroscopies are non-invasive technique and do not require labelling...

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Main Author:	Arshad, Ahmad Zulhimi
Format:	Thesis
Language:	English
Published:	2016
Subjects:	QH Natural history
Online Access:	http://eprints.utm.my/id/eprint/78685/1/AhmadZulhilmiArshadPFBME2016.pdf
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spelling	my-utm-ep.786852018-08-29T07:56:49Z Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy 2016-09 Arshad, Ahmad Zulhimi QH Natural history Terahertz and infrared spectroscopy are effective analytical spectroscopic techniques to identify and study the conformation and molecular interaction of the biomolecules. It has a huge potential in cancer diagnosis because these spectroscopies are non-invasive technique and do not require labelling for tissues and cells. Volatolomics analysis is a technique to analyse the volatile organic compounds (VOCs) emitted and released by human metabolites, which are not limited to breathe analysis. VOCs that are released by cancerous cells can be one of the bio-diagnostics techniques to diagnose cancer. Although studies on breath analysis have been widely carried out, the study of the volatolomics analysis by using Fourier transform infrared spectroscopy (FTIR) and Terahertz time-domain spectroscopy (THz-TDS) is still new. Both FTIR and THz-TDS instruments are installed with a gas cell sampling tools by absorption technique to analyse and detect the key species released from the VOCs. Lung cancer (NCL-H1299) and colon cancer (COLO320DM) cell lines are uas samples to identify the key species of each of the cancerous cells. The experiment has been verified and validated by comparing with control samples such as normal lung (MRC-5) cell lines, normal colon (CCD112CoN) cell lines, empty flask, air from the culture media and normal lab air. All the samples have been cultured into different sealed flasks for 24 to 120 hours, before the VOCs are collected and transferred into the gas cells to analyse using FTIR and THz-TDS. Hydrogen chloride and benzamide have been identified as key species for lung and colon cancer, respectively. These findings have been verified and validated by using residual gas analyser (RGA), gas chromatography – mass selective detector (GC-MSD), and confirmed by earlier literatures. A chemometric statistical analysis also has been applied to this study to extract the important information of the biochemical data from the VOCs with the greatest discriminative power and highest precision. These findings demonstrate the potential use of FTIR and THz-TDS as clinical tools through the volatolomics analysis. In addition, more work is needed if it is to be applied in clinical practice. 2016-09 Thesis http://eprints.utm.my/id/eprint/78685/ http://eprints.utm.my/id/eprint/78685/1/AhmadZulhilmiArshadPFBME2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:106122 phd doctoral University of Bath, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QH Natural history
spellingShingle	QH Natural history Arshad, Ahmad Zulhimi Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
description	Terahertz and infrared spectroscopy are effective analytical spectroscopic techniques to identify and study the conformation and molecular interaction of the biomolecules. It has a huge potential in cancer diagnosis because these spectroscopies are non-invasive technique and do not require labelling for tissues and cells. Volatolomics analysis is a technique to analyse the volatile organic compounds (VOCs) emitted and released by human metabolites, which are not limited to breathe analysis. VOCs that are released by cancerous cells can be one of the bio-diagnostics techniques to diagnose cancer. Although studies on breath analysis have been widely carried out, the study of the volatolomics analysis by using Fourier transform infrared spectroscopy (FTIR) and Terahertz time-domain spectroscopy (THz-TDS) is still new. Both FTIR and THz-TDS instruments are installed with a gas cell sampling tools by absorption technique to analyse and detect the key species released from the VOCs. Lung cancer (NCL-H1299) and colon cancer (COLO320DM) cell lines are uas samples to identify the key species of each of the cancerous cells. The experiment has been verified and validated by comparing with control samples such as normal lung (MRC-5) cell lines, normal colon (CCD112CoN) cell lines, empty flask, air from the culture media and normal lab air. All the samples have been cultured into different sealed flasks for 24 to 120 hours, before the VOCs are collected and transferred into the gas cells to analyse using FTIR and THz-TDS. Hydrogen chloride and benzamide have been identified as key species for lung and colon cancer, respectively. These findings have been verified and validated by using residual gas analyser (RGA), gas chromatography – mass selective detector (GC-MSD), and confirmed by earlier literatures. A chemometric statistical analysis also has been applied to this study to extract the important information of the biochemical data from the VOCs with the greatest discriminative power and highest precision. These findings demonstrate the potential use of FTIR and THz-TDS as clinical tools through the volatolomics analysis. In addition, more work is needed if it is to be applied in clinical practice.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Arshad, Ahmad Zulhimi
author_facet	Arshad, Ahmad Zulhimi
author_sort	Arshad, Ahmad Zulhimi
title	Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
title_short	Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
title_full	Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
title_fullStr	Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
title_full_unstemmed	Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
title_sort	volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy
granting_institution	University of Bath, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/78685/1/AhmadZulhilmiArshadPFBME2016.pdf
_version_	1747818046223810560

Volatolomics analysis of lung and colon cancer using terahertz and infrared spectroscopy

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