Bioremediation of anthracene and remazol brilliant blue R by fungi

Fungi are reported as a potent tool to biodegrade many type of organic pollutants for instance polyaromatic hydrocarbons (PAHs) and synthetic dyes that caused severe pollution to the environment. This research emphasized on biodegradation and biosorption process of PAHs and synthetic dyes; anthracen...

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Main Author:	Mohd. Nor, Nurafifah
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TA Engineering (General) Civil engineering (General)
Online Access:	http://eprints.utm.my/id/eprint/78848/1/NurafifahMohdNorMFKA2016.pdf
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spelling	my-utm-ep.788482018-09-17T04:21:13Z Bioremediation of anthracene and remazol brilliant blue R by fungi 2016-09 Mohd. Nor, Nurafifah TA Engineering (General). Civil engineering (General) Fungi are reported as a potent tool to biodegrade many type of organic pollutants for instance polyaromatic hydrocarbons (PAHs) and synthetic dyes that caused severe pollution to the environment. This research emphasized on biodegradation and biosorption process of PAHs and synthetic dyes; anthracene and Remazol Brilliant Blue R (RBBR) collected from decayed wood and soil. Trichoderma citrinoviride W04, Trichoderma koningiopsis W14 and Pestalotiopsis W15 were selected among twenty fungi species based on faster growth rate after 5 days of incubation. The identification of fungal species was done by morphology characterization and 18S rRNA sequence analysis. According to phylogenetic tree, W04, W14 and W15 were classified as Trichoderma citrinoviride, Trichoderma koningiopsis, and Pestalotiopsis sp. These three fungi species were used further to study the environmental effects; agitation, carbon and nitrogen sources and pH were investigated to choose optimum parameters. Optimization parameters are compulsory to obtain better results. The biodegradation and biosorption process were investigated using UV-Vis spectrophotometer, gas chromatography (GC), Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy (FESEM). The optimum parameters for RBBR by Pestalotiopsis sp. W15 in this study were glucose (86.7%), yeast (91.9%), agitation condition (71.1%) and pH 5 (88.4%). Meanwhile, Trichoderma koningiospsis W14 had the highest degradation of anthracene and optimum in galactose (55.34%), ammonium chloride (55.2%), agitation (46.8%) and pH 5 (26.2%) culture condition. Isotherm and kinetic studies for RBBR and anthracene showed the adsorption process best fit the Langmuir with R2 of 0.93 and 0.90 with maximum biosorption capacity; 0.17 and 0.78 and followed pseudo second order models with rate constant of 0.19 x 0.1/min and 0.47 x 0.1/min. This result demonstrated that fungi are promising biosorbent material to treat RBBR and anthracene. 2016-09 Thesis http://eprints.utm.my/id/eprint/78848/ http://eprints.utm.my/id/eprint/78848/1/NurafifahMohdNorMFKA2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:106435 masters Universiti Teknologi Malaysia, Faculty of Civil Engineering Faculty of Civil Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TA Engineering (General) Civil engineering (General)
spellingShingle	TA Engineering (General) Civil engineering (General) Mohd. Nor, Nurafifah Bioremediation of anthracene and remazol brilliant blue R by fungi
description	Fungi are reported as a potent tool to biodegrade many type of organic pollutants for instance polyaromatic hydrocarbons (PAHs) and synthetic dyes that caused severe pollution to the environment. This research emphasized on biodegradation and biosorption process of PAHs and synthetic dyes; anthracene and Remazol Brilliant Blue R (RBBR) collected from decayed wood and soil. Trichoderma citrinoviride W04, Trichoderma koningiopsis W14 and Pestalotiopsis W15 were selected among twenty fungi species based on faster growth rate after 5 days of incubation. The identification of fungal species was done by morphology characterization and 18S rRNA sequence analysis. According to phylogenetic tree, W04, W14 and W15 were classified as Trichoderma citrinoviride, Trichoderma koningiopsis, and Pestalotiopsis sp. These three fungi species were used further to study the environmental effects; agitation, carbon and nitrogen sources and pH were investigated to choose optimum parameters. Optimization parameters are compulsory to obtain better results. The biodegradation and biosorption process were investigated using UV-Vis spectrophotometer, gas chromatography (GC), Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy (FESEM). The optimum parameters for RBBR by Pestalotiopsis sp. W15 in this study were glucose (86.7%), yeast (91.9%), agitation condition (71.1%) and pH 5 (88.4%). Meanwhile, Trichoderma koningiospsis W14 had the highest degradation of anthracene and optimum in galactose (55.34%), ammonium chloride (55.2%), agitation (46.8%) and pH 5 (26.2%) culture condition. Isotherm and kinetic studies for RBBR and anthracene showed the adsorption process best fit the Langmuir with R2 of 0.93 and 0.90 with maximum biosorption capacity; 0.17 and 0.78 and followed pseudo second order models with rate constant of 0.19 x 0.1/min and 0.47 x 0.1/min. This result demonstrated that fungi are promising biosorbent material to treat RBBR and anthracene.
format	Thesis
qualification_level	Master's degree
author	Mohd. Nor, Nurafifah
author_facet	Mohd. Nor, Nurafifah
author_sort	Mohd. Nor, Nurafifah
title	Bioremediation of anthracene and remazol brilliant blue R by fungi
title_short	Bioremediation of anthracene and remazol brilliant blue R by fungi
title_full	Bioremediation of anthracene and remazol brilliant blue R by fungi
title_fullStr	Bioremediation of anthracene and remazol brilliant blue R by fungi
title_full_unstemmed	Bioremediation of anthracene and remazol brilliant blue R by fungi
title_sort	bioremediation of anthracene and remazol brilliant blue r by fungi
granting_institution	Universiti Teknologi Malaysia, Faculty of Civil Engineering
granting_department	Faculty of Civil Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/78848/1/NurafifahMohdNorMFKA2016.pdf
_version_	1747818086164070400

Bioremediation of anthracene and remazol brilliant blue R by fungi

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