Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity

Moringa oleifera Lam. (M. oleifera) which is also called “pokok kelor” is known to be a rich source of flavonoids. Three flavonoids known as isoquercetin, quercetin and kaempferol were isolated from the ethanolic extract of M. oleifera leaves. The compounds were elucidated using spectroscopic method...

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Main Author:	Mohd. Rozi, Nurshahira
Format:	Thesis
Language:	English
Published:	2017
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/78877/1/NurshahiraMohdRoziMFS2017.pdf
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spelling	my-utm-ep.788772018-09-17T07:15:51Z Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity 2017 Mohd. Rozi, Nurshahira QD Chemistry Moringa oleifera Lam. (M. oleifera) which is also called “pokok kelor” is known to be a rich source of flavonoids. Three flavonoids known as isoquercetin, quercetin and kaempferol were isolated from the ethanolic extract of M. oleifera leaves. The compounds were elucidated using spectroscopic methods such as 1H NMR, 13C NMR and FTIR. In a separate study, the different extraction methods of ethanolic extract including cold maceration, soxhlet, ultrasound-assisted by water bath and ultrasound-assisted by probe and solid phase extraction were done. The extracts were subjected to qualitative and quantitative analysis to determine the phytochemicals present in M. oleifera leaf. Qualitative analysis on the extracts showed that M. oleifera contains flavonoid, tannin, alkaloid, phenol, steroid, quinone and coumarin. The result of quantitative analysis was obtained by screening the ethanolic extract for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl assay, 2,2'azino bis(3-ethylbenzothiozoline-6-sulfonic acid) and ferric reducing antioxidant power assay. Extract from Fraction 2 of solid phase exhibited highest antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azinobis(3-ethylbenzothiozoline-6-sulfonic acid) and ferric reducing antioxidant power assay with IC50 of 35.81 µg/mL, IC50 of 41.58 µg/mL and FRAP equivalent of 91.36 mM, respectively. Moreover, the extracts were further analysed using a reversed phase high performance liquid chromatography to quantify the contents of isoquercetin, quercetin and kaempferol. Quantification of isoquercetin, quercetin and kaempferol were found higher using solid phase extraction with 7.98%, 0.86% and 1.11% in w/w%, respectively. The validated HPLC method was effective and practical for quantification of isoquercetin, quercetin and kaempferol in M. oleifera leaf extracts. 2017 Thesis http://eprints.utm.my/id/eprint/78877/ http://eprints.utm.my/id/eprint/78877/1/NurshahiraMohdRoziMFS2017.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:106937 masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Mohd. Rozi, Nurshahira Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
description	Moringa oleifera Lam. (M. oleifera) which is also called “pokok kelor” is known to be a rich source of flavonoids. Three flavonoids known as isoquercetin, quercetin and kaempferol were isolated from the ethanolic extract of M. oleifera leaves. The compounds were elucidated using spectroscopic methods such as 1H NMR, 13C NMR and FTIR. In a separate study, the different extraction methods of ethanolic extract including cold maceration, soxhlet, ultrasound-assisted by water bath and ultrasound-assisted by probe and solid phase extraction were done. The extracts were subjected to qualitative and quantitative analysis to determine the phytochemicals present in M. oleifera leaf. Qualitative analysis on the extracts showed that M. oleifera contains flavonoid, tannin, alkaloid, phenol, steroid, quinone and coumarin. The result of quantitative analysis was obtained by screening the ethanolic extract for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl assay, 2,2'azino bis(3-ethylbenzothiozoline-6-sulfonic acid) and ferric reducing antioxidant power assay. Extract from Fraction 2 of solid phase exhibited highest antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azinobis(3-ethylbenzothiozoline-6-sulfonic acid) and ferric reducing antioxidant power assay with IC50 of 35.81 µg/mL, IC50 of 41.58 µg/mL and FRAP equivalent of 91.36 mM, respectively. Moreover, the extracts were further analysed using a reversed phase high performance liquid chromatography to quantify the contents of isoquercetin, quercetin and kaempferol. Quantification of isoquercetin, quercetin and kaempferol were found higher using solid phase extraction with 7.98%, 0.86% and 1.11% in w/w%, respectively. The validated HPLC method was effective and practical for quantification of isoquercetin, quercetin and kaempferol in M. oleifera leaf extracts.
format	Thesis
qualification_level	Master's degree
author	Mohd. Rozi, Nurshahira
author_facet	Mohd. Rozi, Nurshahira
author_sort	Mohd. Rozi, Nurshahira
title	Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
title_short	Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
title_full	Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
title_fullStr	Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
title_full_unstemmed	Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
title_sort	isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2017
url	http://eprints.utm.my/id/eprint/78877/1/NurshahiraMohdRoziMFS2017.pdf
_version_	1747818093334233088

Isolation and quantification of flavonoids from leaves of moringa oleifera lam and its antioxidant activity

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