Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria

Oil palm is the biggest commodity crop while banana is the second largest fruit crop planted in Malaysia. Both are highly nutrient-demanding crops that requires a large amount of fertilizer. This present an opportunity to find alternative source of nutrient that is much cheaper than the imported ino...

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Main Author:	Then, Kek Hoe
Format:	Thesis
Language:	English
Published:	2016
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/79000/1/ThenKekHoePFChE2016.pdf
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spelling	my-utm-ep.790002018-09-19T05:22:47Z Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria 2016 Then, Kek Hoe TP Chemical technology Oil palm is the biggest commodity crop while banana is the second largest fruit crop planted in Malaysia. Both are highly nutrient-demanding crops that requires a large amount of fertilizer. This present an opportunity to find alternative source of nutrient that is much cheaper than the imported inorganic fertilizer for oil palm and banana plantation. Hence, the objective of this study was to develop a newly formulated biofertilizer incorporating locally isolated indigenous nitrogen fixing bacteria (NFB) and phosphate solubilising bacteria (PSB) into oil palm empty fruit bunch (EFB) compost as alternative nutrient source for banana and oil palm to improve their growth and nutrient uptake. Three NFB and PSB strains (Enterobacter cloacae, Burkholderia cepacia and Serratia marcescens) were successfully isolated from the root of oil palm and banana to be formulated as biofertilizer for evaluation on oil palm and banana seedlings. The formulated biofertilizer showed high viable cell count of NFB and PSB inoculants in the carrier (fine EFB compost) i.e 2.45 x 108 cfu/g to 4.31 x 109 cfu (colony forming unit)/g carrier was recorded at the second day after inoculation. Biofertilizer formulation containing Enterobacter cloacae showed promising and consistent effect on the growth of oil palm and banana seedlings including increased plant height (13.7% for oil palm and 15.6% for banana), improved total dry biomass production (27.4% and 33.8% respectively) and high total nutrient uptake (nitrogen (N) 30.6-48.1%, phosphorous (P) 27.2-33.1%, potassium (K) 30.5-37.7%, magnesium (Mg) 48.8%, sulfur (S) 18.1-28.5% and boron (B) 24.5%). Enterobacter cloaceae also showed good root colonization ability as shown from the Field emission scanning electron microscopy analysis. The use of nutrient-rich EFB compost (3.06% N, 0.37% P, 4.74% K, 3.32% calcium (Ca), 0.79% Mg, 0.07% S, 70.3 mg/kg B, 102.1 mg/kg copper (Cu), 6600.9 mg/kg iron (Fe), 160.2 mg/kg zinc (Zn), 455.2 mg/kg manganese (Mn) and 41.4% total carbon, C) as carrier for biofertilizer was able to improve the soil properties notably soil pH, organic C, total N, total P, available P and exchangeable Mg. As a conclusion, the application of locally isolated NFB and PSB together with EFB as carrier (as well as source of nutrient) was successful to improve the growth of oil palm and banana seedlings together with the soil properties. Biofertilizer formulation containing Enterobacter cloacae showed higher performance relative to the other formulations, hence recommended to be applied as soil mixture or directly applied into the planting hole of oil palm and banana seedlings during transplanting to the field to encourage the growth and nutrient uptake during early planting phase. 2016 Thesis http://eprints.utm.my/id/eprint/79000/ http://eprints.utm.my/id/eprint/79000/1/ThenKekHoePFChE2016.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:106287 phd doctoral Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Then, Kek Hoe Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
description	Oil palm is the biggest commodity crop while banana is the second largest fruit crop planted in Malaysia. Both are highly nutrient-demanding crops that requires a large amount of fertilizer. This present an opportunity to find alternative source of nutrient that is much cheaper than the imported inorganic fertilizer for oil palm and banana plantation. Hence, the objective of this study was to develop a newly formulated biofertilizer incorporating locally isolated indigenous nitrogen fixing bacteria (NFB) and phosphate solubilising bacteria (PSB) into oil palm empty fruit bunch (EFB) compost as alternative nutrient source for banana and oil palm to improve their growth and nutrient uptake. Three NFB and PSB strains (Enterobacter cloacae, Burkholderia cepacia and Serratia marcescens) were successfully isolated from the root of oil palm and banana to be formulated as biofertilizer for evaluation on oil palm and banana seedlings. The formulated biofertilizer showed high viable cell count of NFB and PSB inoculants in the carrier (fine EFB compost) i.e 2.45 x 108 cfu/g to 4.31 x 109 cfu (colony forming unit)/g carrier was recorded at the second day after inoculation. Biofertilizer formulation containing Enterobacter cloacae showed promising and consistent effect on the growth of oil palm and banana seedlings including increased plant height (13.7% for oil palm and 15.6% for banana), improved total dry biomass production (27.4% and 33.8% respectively) and high total nutrient uptake (nitrogen (N) 30.6-48.1%, phosphorous (P) 27.2-33.1%, potassium (K) 30.5-37.7%, magnesium (Mg) 48.8%, sulfur (S) 18.1-28.5% and boron (B) 24.5%). Enterobacter cloaceae also showed good root colonization ability as shown from the Field emission scanning electron microscopy analysis. The use of nutrient-rich EFB compost (3.06% N, 0.37% P, 4.74% K, 3.32% calcium (Ca), 0.79% Mg, 0.07% S, 70.3 mg/kg B, 102.1 mg/kg copper (Cu), 6600.9 mg/kg iron (Fe), 160.2 mg/kg zinc (Zn), 455.2 mg/kg manganese (Mn) and 41.4% total carbon, C) as carrier for biofertilizer was able to improve the soil properties notably soil pH, organic C, total N, total P, available P and exchangeable Mg. As a conclusion, the application of locally isolated NFB and PSB together with EFB as carrier (as well as source of nutrient) was successful to improve the growth of oil palm and banana seedlings together with the soil properties. Biofertilizer formulation containing Enterobacter cloacae showed higher performance relative to the other formulations, hence recommended to be applied as soil mixture or directly applied into the planting hole of oil palm and banana seedlings during transplanting to the field to encourage the growth and nutrient uptake during early planting phase.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Then, Kek Hoe
author_facet	Then, Kek Hoe
author_sort	Then, Kek Hoe
title	Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
title_short	Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
title_full	Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
title_fullStr	Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
title_full_unstemmed	Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
title_sort	improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2016
url	http://eprints.utm.my/id/eprint/79000/1/ThenKekHoePFChE2016.pdf
_version_	1747818123542659072

Improving banana and oil palm seedlings growth using indigenous nitrogen fixing and phospate solubilizing bacteria

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